• MeSH
• krysa rodu rattus MeSH
• svaly inervace   transplantace   MeSH
• Check Tag
• krysa rodu rattus MeSH

• Publikační typ
• abstrakt z konference MeSH

This study aimed to investigate whether heat stress (HS) prevents a decrease in succinate dehydrogenase (SDH) activity and heat shock protein 60 (HSP60) and superoxide dismutase 2 (SOD2) contents in the extensor digitorum longus of streptozotocin (STZ)-induced diabetic rats. Twelve-week-old male Wistar rats were assigned to one of the four groups (n=6/group): control (Con), HS, diabetes mellitus (DM), and diabetes mellitus and heat stress (DM+HS). Diabetes was induced by the administration of STZ (50 mg/kg). HS was initiated 7 days after STZ treatment and performed at 42 °C for 30 min 5 times a week for 3 weeks. SDH activity was decreased in the DM and DM+HS groups. However, SDH activity was greater in the DM+HS group than in the DM group. Although HSP60 content was lower in the DM group than in the Con group, it was maintained in the DM+HS groups and was higher than that in the DM group. SOD2 content was decreased only in the DM group. These findings suggest that HS prevents the decrease in SDH activity in the skeletal muscle induced by DM. According to this mechanism, the maintenance of SOD2 and HSP60 by HS may suppress the increase in oxidative stress.

• MeSH
• aktivace enzymů fyziologie   MeSH
• chaperon hsp60 metabolismus   MeSH
• experimentální diabetes mellitus metabolismus   MeSH
• kosterní svaly enzymologie   metabolismus   MeSH
• krevní glukóza metabolismus   MeSH
• krysa rodu rattus MeSH
• mitochondriální proteiny metabolismus   MeSH
• potkani Wistar MeSH
• reakce na tepelný šok fyziologie   MeSH
• streptozocin MeSH
• sukcinátdehydrogenasa metabolismus   MeSH
• superoxiddismutasa metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

A novel derivative of 6-methyluracil, C-547, increased the amplitude and prolonged the duration of miniature endplate currents (MEPCs) which is typical for acetylcholinesterase inhibition. In the soleus and extensor digitorum longus significant potentiation was detected at nanomolar concentrations. In contrast, in the diaphragm muscle, the increase in the amplitudes of the MEPCs and the decay time constant ? appeared only when the concentration of C-547 was elevated to 1x10-7 M. Possible consequences for the exploitation of this drug, which can selectively inhibit AChE in particular synapses, are discussed.

• MeSH
• acetylcholinesterasa farmakokinetika   účinky léků   MeSH
• bránice fyziologie   účinky léků   MeSH
• cholinesterasové inhibitory farmakokinetika   chemie   MeSH
• elektrofyziologie metody   statistika a číselné údaje   MeSH
• financování vládou MeSH
• interpretace statistických dat MeSH
• nervosvalová ploténka enzymologie   fyziologie   chemie   MeSH
• potkani Wistar fyziologie   MeSH
• svaly fyziologie   účinky léků   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• srovnávací studie MeSH

• MeSH
• elektrická stimulace MeSH
• estrogeny MeSH
• kosterní svaly fyziologie   MeSH
• krysa rodu rattus MeSH
• pohlaví MeSH
• svalová kontrakce MeSH
• svalová únava fyziologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• srovnávací studie MeSH

The aim of this study was to determine whether capillarity in the denervated and reinnervated rat extensor digitorum longus muscle (EDL) is scaled by muscle fiber oxidative potential. We visualized capillaries adjacent to a metabolically defined fiber type and estimated capillarity of fibers with very high oxidative potential (O) vs fibers with very low oxidative potential (G). Capillaries and muscle fiber types were shown by a combined triple immunofluorescent technique and the histochemical method for NADH-tetrazolium reductase. Stacks of images were captured by a confocal microscope. Applying the Ellipse program, fibers were outlined, and the diameter, perimeter, cross-sectional area, length, surface area, and volume within the stack were calculated for both fiber types. Using the Tracer plug-in module, capillaries were traced within the three-dimensional (3D) volume, the length of capillaries adjacent to individual muscle fibers was measured, and the capillary length per fiber length (Lcap/Lfib), surface area (Lcap/Sfib), and volume (Lcap/Vfib) were calculated. Furthermore, capillaries and fibers of both types were visualized in 3D. In all experimental groups, O and G fibers significantly differed in girth, Lcap/Sfib, and Lcap/Vfib, but not in Lcap/Lfib. We conclude that capillarity in the EDL is scaled by muscle fiber size and not by muscle fiber oxidative potential.

• MeSH
• denervace svalu MeSH
• financování organizované MeSH
• histocytochemie MeSH
• kapiláry anatomie a histologie   MeSH
• konfokální mikroskopie MeSH
• kosterní svalová vlákna metabolismus   MeSH
• kosterní svaly inervace   krevní zásobení   metabolismus   MeSH
• krysa rodu rattus MeSH
• oxidace-redukce MeSH
• potkani Wistar MeSH
• zobrazování trojrozměrné MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH

We examined the effects of the unilateral heterochronous isotransplantation on the fiber type composition and myosin heavy chain (MyHC) isoform content of unoperated slow soleus and fast extensor digitorum longus muscles of female inbred Lewis strain rats. Comparison was made between "control" unoperated muscles of experimental rats (after intramuscular transplantation surgery) with the corresponding muscles of completely naive (unoperated) rats of three age groups (5-, 8- and 14-month-old). This was done in order to ascertain whether these muscles can be used as reliable controls to the transplanted and host muscles for our ongoing grafting experiments. The fiber type composition was determined by assessing the histochemical reaction for myofibrillar adenosine triphosphatase, the MyHC isoform content was determined immunocytochemically using monoclonal antibodies specific to different MyHC isoforms and by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Our experiments show that the heterochronous intramuscular isotransplantation procedure had no significant effect on the fiber type composition and MyHC isoform content of the "control" unoperated muscles of the experimental rats when compared to the corresponding muscles of the naive animals. Furthermore, the duration and type of isotransplantation did not also lead to differences among corresponding "control" muscles of experimental animals. We conclude that the unoperated muscles of the experimental rats can be used as controls in our current transplantation project dealing with long-term grafting experiments.

• MeSH
• adenosintrifosfatasy metabolismus   MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• financování organizované MeSH
• homologní transplantace MeSH
• imunohistochemie MeSH
• isomerie MeSH
• kosterní svaly cytologie   transplantace   MeSH
• krysa rodu rattus MeSH
• myofibrily metabolismus   MeSH
• potkani inbrední LEW MeSH
• přežívání štěpu MeSH
• svalová vlákna typu I cytologie   metabolismus   MeSH
• svalová vlákna typu II cytologie   metabolismus   MeSH
• těžké řetězce myosinu chemie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• ženské pohlaví MeSH
• zvířata MeSH

• MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• imunohistochemie metody   MeSH
• kosterní svalová vlákna anatomie a histologie   MeSH
• kosterní svaly anatomie a histologie   MeSH
• krysa rodu rattus MeSH
• svalová vlákna typu I MeSH
• svalová vlákna typu II MeSH
• svalové proteiny MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• ženské pohlaví MeSH
• zvířata MeSH

Derivative of 6-methyluracil, selective cholinesterase inhibitor C-547 potentiates miniature endplate currents (MEPCs) in rat external intercostal muscles (external ICM) more effectively than in internal intercostal muscles (internal ICM). Effect of the C-547 on intercostal muscles was compared with those on extensor digitorum longus (EDL) and diaphragm muscles. Half-effective concentrations for tau of MEPC decay arranged in increasing order were as follows: EDL, locomotor muscle, most sensitive = 1.3 nM, external ICM, inspiration muscle = 6.8 nM, diaphragm, main inspiration muscle = 28 nM, internal ICM, expiration muscle = 71 nM. External ICM might therefore be inhibited, similarly as the limb muscles, by nanomolar concentrations of the drug and do not participate in inspiration in the presence of the C-547. Moreover, internal ICM inhibition can hinder the expiration during exercise-induced fast breathing of C-547- treated experimental animals.

• MeSH
• bránice enzymologie   účinky léků   MeSH
• časové faktory MeSH
• cholinesterasové inhibitory farmakologie   MeSH
• financování organizované MeSH
• kosterní svaly enzymologie   účinky léků   MeSH
• krysa rodu rattus MeSH
• kvartérní amoniové sloučeniny farmakologie   MeSH
• mechanika dýchání účinky léků   MeSH
• mezižeberní svaly enzymologie   účinky léků   MeSH
• miniaturní postsynaptické potenciály účinky léků   MeSH
• potkani Wistar MeSH
• uracil analogy a deriváty   farmakologie   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH

• MeSH
• acetylcholin analýza   MeSH
• bránice chemie   MeSH
• krysa rodu rattus MeSH
• přijímání potravy MeSH
• Check Tag
• krysa rodu rattus MeSH