• MeSH
• elektronová mikroskopie metody   MeSH
• fluorescenční protilátková technika MeSH
• imunologické testy MeSH
• RNA-viry imunologie   izolace a purifikace   ultrastruktura   MeSH
• skot MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH

• MeSH
• antisérum diagnostické užití   MeSH
• elektronová mikroskopie využití   MeSH
• virové nemoci diagnóza   ultrastruktura   MeSH
• viry ultrastruktura   MeSH

• MeSH
• elektronová mikroskopie MeSH
• imunokomplex MeSH
• králíci MeSH
• šok patologie   MeSH
• Check Tag
• králíci MeSH

• MeSH
• elektronová mikroskopie MeSH
• ferritiny MeSH
• histocytochemie MeSH
• imunokomplex MeSH
• slezina imunologie   MeSH

• MeSH
• centrální nervový systém patologie   ultrastruktura   MeSH
• elektronová mikroskopie MeSH
• imunizace MeSH
• vzteklina etiologie   imunologie   komplikace   MeSH

Analysis of the tissue harvested around the total hip replacement isolated from re-operated patients in order to: (1) characterize complexity of structural processes developing in the region of the total hip replacement and, (2) to define the role and significance of histological structures of this tissue, mainly in relation to implant loosening, from the viewpoint of formal and causal pathogenesis. MATERIAL AND METHODS Biopsied material isolated from periarticular tissue of re-operated patients (n=19) after THR was analyzed using the methods of light optic, fluorescent (TUNEL), and transmission electron microscopy. RESULTS Histological analysis revealed fibroproliferaive processes and epithelioid granulomatosis cell reaction around the implant with the formation of giant multinuclear syncythial (osteoclastlike) structures as a response to foreign bodies. These structures phagocyte fragments of foreign material (polyethylene particles from the implant, cement fragments). All the used methods revealed a range of regressive changes in the layers of foreign microparticles (inside giant multinuclear cells) typical of fibrinoid necrosis in collagen fibres and apoptosis. In certain cases, progressive changes as chondroid and synovial differentiation (metaplasia) were observed. DISCUSSION Total hip replacement, despite all positive aspects for patients, may cause permanent inflammatory processes in its surrounding. This may result in an extensive fibroproduction of a differently thick layer of connective tissue around the implant. An important factor of loosening of THR is probably osteoclastic resorption in the area of "bone-implant" interface, as a result of the interaction between the inflammatory mechanisms around the implant. CONCLUSION In the postoperative period, there occur fibroproliferative changes in the periarticular tissue and large population of multinuclear cells. In our view, these cells play a role in the production of wear particles from the implant and microparticles of bone tissues and bone cement. Fibroproliferative process may be considered as an immune response to the implanted foreign material.

• MeSH
• granulační tkáň patologie   ultrasonografie   MeSH
• koncové značení zlomů DNA in situ MeSH
• kyčelní kloub patologie   MeSH
• kyčelní protézy MeSH
• lidé středního věku MeSH
• lidé MeSH
• mikroskopie elektronová rastrovací transmisní MeSH
• náhrada kyčelního kloubu MeSH
• reakce na cizí těleso patologie   MeSH
• reoperace MeSH
• selhání protézy MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH

• MeSH
• elektronová mikroskopie MeSH
• hepatitida B - antigeny MeSH
• imunokomplex MeSH

• MeSH
• antigeny MeSH
• buněčné jádro MeSH
• cytoplazma MeSH
• elektronová mikroskopie MeSH
• Golgiho aparát MeSH
• imunologická tolerance MeSH
• lidé MeSH
• myši MeSH
• paralýza imunologie   MeSH
• plazmatické buňky cytologie   MeSH
• sérový albumin MeSH
• slezina cytologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH

Intramuscular administration of the filtrate of organ suspensions, prepared from a dead rabbit, killed 62.9% of inoculated rabbits within 1 to 5 days, while 93.3% died after intranasal administration of the same inoculum. The virus survived freeze-drying and was resistant to treatment with 0.4% formaldehyde when incubated at 37 degrees C for 1 hour and 4 degrees C for the subsequent 12 hours, but lost its infectivity when the treatment was prolonged to 3 hours at 37 degrees C and 3 days at room temperature. Its infectivity was also inhibited by reconvalescent serum. The virus could not be detected after 3 passages in primary rabbit kidney cell cultures. Electron microscopy of negatively stained preparations demonstrated icosahedral virus particles with a diameter of 29 to 33 nm without an envelope. Accurate morphological classification has not yet been completed. Incubation with a reconvalescent serum, diluted 1:20 or 1:40, resulted in the formation of immune complexes, detectable by electron microscopy.

• MeSH
• Caliciviridae ultrastruktura   MeSH
• elektronová mikroskopie MeSH
• hemoragické horečky virové přenos   veterinární   MeSH
• králíci * MeSH
• pikornavirové infekce přenos   veterinární   MeSH
• zvířata MeSH
• Check Tag
• králíci * MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Československo MeSH