metalloporphyrin Dotaz Zobrazit nápovědu
- MeSH
- hem MeSH
- kolorektální nádory metabolismus MeSH
- lidé MeSH
- metaloporfyriny MeSH
- oxygenasy MeSH
- Check Tag
- lidé MeSH
Metalloporphyrins are an important group of sensitizers with a porphyrin skeleton. Their photophysical properties are significantly affected by the nature of the central ion. In this work, we focus on the mechanical properties of a cervix carcinoma cell line which underwent photodynamic treatment (PDT) with MgTPPS4 photosensitzer. Atomic force microscopy alongside confocal microscopy was used to quantify and qualify the structural characteristics before and after PDT. Cells before PDT showed a fine actin network and higher elasticity with the median of Young modulus 12.2 kPa. After PDT, the median of Young modulus was 13.4 kPa and a large redistribution in the actin network was observed.
- MeSH
- cytoskelet účinky léků fyziologie účinky záření MeSH
- fotosenzibilizující látky chemie farmakologie MeSH
- HeLa buňky MeSH
- konfokální mikroskopie MeSH
- lidé MeSH
- metaloporfyriny chemie farmakologie MeSH
- mikroskopie atomárních sil MeSH
- modul pružnosti MeSH
- světlo MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Photodynamic therapy (PDT) is one of the treatments for cancer. This therapy uses a combination of a photosensitizer (PS), light irradiation, and oxygen O2, which is converted to cytotoxic 1O2 and other forms of reactive oxygen species (ROS), causing selective damage to the target tissue. In this work, we studied effect of two porphyrin photosensitizers TMPyP and ZnTPPS4 at three different concentrations (0.25, 0.5, 5μM) after two irradiation doses (5 and 25 J/cm2). Photodynamic efect of TMPyP and ZnTPPS4 were confirmed by a battery of in vitro tests including MTT, reactive oxygen species (ROS) production and mitochondrial membrane potential test (MMP). Morphological changes of the cells before and after treatment were imaged by atomic force microscopy (AFM). The most effective combination of irradiation dose and concentration for both PSs showed a concentration of 5 μM and a irradiation dose of 25 J/cm2 in both cell cultures.
- MeSH
- buněčná smrt MeSH
- buněčné linie účinky léků účinky záření MeSH
- fluorescenční mikroskopie MeSH
- fotochemoterapie metody MeSH
- fotosenzibilizující látky farmakologie chemie MeSH
- lasery MeSH
- lidé MeSH
- metaloporfyriny farmakologie chemie MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Přeruš. str. : il. ; 30 cm
Vzhledem k výsledkům získaným studiem účinku inhib.hemoxygenasy, Zn-DPBG,na proliferaci lidského Ca colon,je nutné rozšířit projekt o sledování účinku indukt.syntézy hemoxygenasy a inhibit.NOS.
- MeSH
- experimentální nádory MeSH
- hemová oxygenasa (decyklizující) MeSH
- invazivní růst nádoru MeSH
- kolorektální nádory MeSH
- metaloporfyriny MeSH
- thymidinkináza MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- onkologie
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
The spatiotemporal sensing of specific cationic and anionic species is crucial for understanding the processes occurring in living systems. Herein, we developed new fluorescence sensors derived from tetrapyrazinoporphyrazines (TPyzPzs) with a recognition moiety that consists of an aza-crown and supporting substituents. Their sensitivity and selectivity were compared by fluorescence titration experiments with the properties of known TPyzPzs (with either one aza-crown moiety or two of these moieties in a tweezer arrangement). Method of standard addition was employed for analyte quantification in saliva. For K+ recognition, the new derivatives had comparable or larger association constants with larger fluorescence enhancement factors compared to that with one aza-crown. Their fluorescence quantum yields in the ON state were 18× higher than that of TPyzPzs with a tweezer arrangement. Importantly, the sensitivity toward cations was strongly dependent on counteranions and increased as follows: NO3- < Br- < CF3SO3- < ClO4- ≪ SCN-. This trend resembles the chaotropic ability expressed by the Hofmeister series. The high selectivity toward KSCN was explained by synergic association of both K+ and SCN- with TPyzPz sensors. The sensing of SCN- was further exploited in a proof of concept study to quantify SCN- levels in the saliva of a smoker and to demonstrate the sensing ability of TPyzPzs under in vitro conditions.
- MeSH
- crown ethery chemická syntéza chemie MeSH
- draslík analýza MeSH
- fluorescence MeSH
- fluorescenční barviva chemická syntéza chemie MeSH
- fluorescenční mikroskopie metody MeSH
- fluorescenční spektrometrie metody MeSH
- HeLa buňky MeSH
- kuřáci MeSH
- lidé MeSH
- limita detekce MeSH
- metaloporfyriny chemická syntéza chemie MeSH
- sliny chemie MeSH
- thiokyanatany analýza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
