Evaluation of basic mitochondrial functions using rat tissue homogenates
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21664301
DOI
10.1016/j.mito.2011.05.006
PII: S1567-7249(11)00214-5
Knihovny.cz E-resources
- MeSH
- Citrate (si)-Synthase metabolism MeSH
- Electron Transport Chain Complex Proteins metabolism MeSH
- Enzyme Assays MeSH
- Liver enzymology metabolism MeSH
- Rats MeSH
- Kidney enzymology metabolism MeSH
- Membrane Potential, Mitochondrial MeSH
- Mitochondrial Proteins metabolism MeSH
- Mitochondria enzymology metabolism MeSH
- Brain enzymology metabolism MeSH
- Myocardium enzymology metabolism MeSH
- Oxidative Phosphorylation MeSH
- Rats, Wistar MeSH
- Electron Transport Complex IV metabolism MeSH
- Oxygen Consumption MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Citrate (si)-Synthase MeSH
- Electron Transport Chain Complex Proteins MeSH
- Mitochondrial Proteins MeSH
- Electron Transport Complex IV MeSH
The primary attempt in diagnostic and experimental studies of numerous pathological states associated with mitochondrial dysfunction is a precise evaluation of changes in function, content and structure of mitochondrial OXPHOS system. The analysis of rat heart, liver, brain and kidney by oxygraphy, enzyme activities, membrane potential, and BN/SDS-PAGE western blotting demonstrated that tissue homogenates can substitute for isolated mitochondria, providing comparable qualitative mitochondrial parameters. The use of homogenate avoids the loss of the majority of mitochondria during their isolation. Only 50-100mg of the tissue is required for the complex OXPHOS analysis, i.e. five times less as compared with isolated mitochondria.
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