Nanobiosynthesis belongs to the most recent methods for synthesis of nanoparticles. This type of synthesis provides many advantages including the uniformity in particle shape and size. The biosynthesis has also a significant advantage regarding chemical properties of the obtained particles. In this study, we characterized the basic properties and composition of quantum dots (QDs), obtained by the extracellular biosynthesis by Escherichia coli. Furthermore, the toxicity of the biosynthesized QDs was compared to QDs prepared by microwave synthesis. The obtained results revealed the presence of cyan CdTe QDs after removal of substantial amounts of organic compounds, which stabilized the nanoparticle surface. QDs toxicity was evaluated using three cell lines Human Foreskin Fibroblast (HFF), Human Prostate Cancer cells (PC-3) and Breast Cancer cells (MCF-7) and the MTT assay. The test revealed differences in the toxicity between variants of QDs, varying about 10% in the HFF and 30% in the MCF-7 cell lines. The toxicity of the biosynthesized QDs to the PC-3 cell lines was about 35% lower in comparison with the QDs prepared by microwave synthesis.
- MeSH
- buněčné linie MeSH
- Escherichia coli metabolismus MeSH
- kvantové tečky toxicita MeSH
- lidé MeSH
- sloučeniny kadmia toxicita MeSH
- telur toxicita MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (γ-glutamylcysteine synthase-GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli showed significant differences in resistance to antibiotics. According to the determined resistance, E. coli were divided into experimental groups based on a statistical evaluation of their properties as more resistant and more sensitive. These groups were also used for finding the differences in a dependence of the glutathione pathway on resistance to antibiotics. More sensitive E. coli showed the same kinetics of glutathione synthesis while blocking GCL (Km 0.1 µM), as compared to non-blocking. In addition, the most frequent mutations in genes of glutathione synthetase, glutathione peroxidase and glutathione reductase were observed in this group compared to laboratory E.coli. The group of "more resistant" E. coli exhibited differences in Km between 0.3 and 0.8 µM. The number of mutations compared to the laboratory E. coli was substantially lower compared to the other group.
- MeSH
- bakteriální léková rezistence účinky léků genetika MeSH
- buthionin sulfoximin farmakologie MeSH
- Escherichia coli účinky léků genetika MeSH
- glutathion genetika MeSH
- glutathionperoxidasa genetika MeSH
- glutathionreduktasa genetika MeSH
- glutathionsynthasa genetika MeSH
- kinetika MeSH
- lidé MeSH
- mutace účinky léků genetika MeSH
- signální transdukce účinky léků genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
Platinum-based cytostatics, such as cisplatin, carboplatin or oxaliplatin are widely used agents in the treatment of various types of tumors. Large amounts of these drugs are excreted through the urine of patients into wastewaters in unmetabolised forms. This phenomenon leads to increased amounts of platinum ions in the water environment. The impacts of these pollutants on the water ecosystem are not sufficiently investigated as well as their content in water sources. In order to facilitate the detection of various types of platinum, we have developed a new, rapid, screening flow injection analysis method with electrochemical detection (FIA-ED). Our method, based on monitoring of the changes in electrochemical behavior of analytes, maintained by various pH buffers (Britton-Robinson and phosphate buffer) and potential changes (1,000, 1,100 and 1,200 mV) offers rapid and cheap selective determination of platinum-based cytostatics and platinum chlorides, which can also be present as contaminants in water environments.
Tripeptide glutathion is one of the most important thiol compound. It is involved in a wide variety of cellular processes. In the organism it occurs in two forms: as a reduced glutathion (GSH) and as an oxidized glutathion (GSSG). Protective and regulatory functions of GSH are based on changes in its redox state. GSH and GSSG forms together one of the most significant redox couples in the cell. Their ratio under the physiological conditions is usually constant, regardless of the total concentration of glutathion in a cell. The ratio of both forms of glutathion is considered as an indicator of oxidative stress effect.
- Klíčová slova
- redoxní pár,
- MeSH
- biologické markery krev MeSH
- glutathion * fyziologie metabolismus MeSH
- lidé MeSH
- oxidace-redukce MeSH
- oxidační stres MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Organisms can produce substances which act as protection against the negative effects of microorganisms. Many of these substances belong among the peptides and represent an important mechanism of innate immunity. Peptides having activity against bacteria, fungi, parasites, viruses and cancer are collectively referred to as antimicrobial peptides (AMPs). Due to the increasing resistance of pathogenic microorganisms to antibiotic treatment, AMPs come to the forefront as a suitable alternative. Out of an antimicrobial activity, also the possible use for the treatment of fungal, parasite and virus diseases are studied. Also it has a significant potential use for the treatment of cancer, as some AMPs demonstrated significant antitumor activity.
- Klíčová slova
- protinádorová aktivita, antimikrobiální aktivita,
- MeSH
- antibakteriální látky * MeSH
- antifungální látky MeSH
- antimikrobiální peptidy MeSH
- antiparazitární látky MeSH
- antivirové látky MeSH
- kationické antimikrobiální peptidy farmakologie MeSH
- lidé MeSH
- peptidy * farmakologie MeSH
- protinádorové látky * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
- Publikační typ
- abstrakt z konference MeSH
OBJECTIVES: Toxic effects of the yew have been known since ancient times. Yew toxicity is due to the content of cyanogenic glycosides and a mixture of alkaloids known as taxines. Taxine B is probably responsible for the most part of adverse effects in poisoned organisms. This particular taxoid is common in body fluids of the yew-poisoned. The present study is engaged with laboratory examination to confirm substances that lead to fatality of a pair of olive baboons (Papio anubis) following ingestion of yew seeds. When both cage mates (male and female) died suddenly, poisoning was suspected because many berries had fallen into the cage from a nearby fruiting yew tree (Taxus baccata) during the windy night before. METHODS: The analysis was performed using electrospray ionization quadrupole time-of-flight mass spectrometry. A flow injection analysis/mass spectrometry setting was prepared for this purpose. RESULTS: The above mentioned mass spectrometry analysis of taxoids confirmed poisoning by taxanes. The presence of taxin B/isotaxin B was confirmed in all investigated samples. Apparently in urine and bile there were concentrations ranging 150-220 ng.mL-1 and in blood serum concentrations 25-30 ng.mL-1. CONCLUSION: It follows from the results obtained that we confirmed that baboons were deadly intoxicated by yew fruits.
- MeSH
- alkaloidy analýza MeSH
- biochemická analýza krve veterinární MeSH
- fatální výsledek MeSH
- klinické laboratorní techniky MeSH
- nemoci opic diagnóza etiologie MeSH
- nemoci přenášené potravou diagnóza veterinární MeSH
- Papio anubis * krev moč MeSH
- taxoidy analýza MeSH
- tis otrava MeSH
- žluč chemie MeSH
- zvířata v ZOO * MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- práce podpořená grantem MeSH
- Publikační typ
- abstrakt z konference MeSH
