There is ample evidence on the benefit of angiotensin receptor-neprilysin inhibitors (ARNIs) in heart failure, yet data regarding the potential protective action of ARNIs in hypertensive heart disease are sparse. The aim of this study was to show whether an ARNI exerts a protective effect in a model of Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertension with a hypertensive heart and to compare this potential benefit with an angiotensin-converting enzyme inhibitor, captopril. Five groups of adult male Wistar rats were studied (14 per group) for four weeks: untreated controls; ARNI (68 mg/kg/day); L-NAME (40 mg/kg/day); L-NAME treated with ARNI; and L-NAME treated with captopril (100 mg/kg/day). L-NAME administration induced hypertension, accompanied by increased left ventricular (LV) weight and fibrotic rebuilding of the LV in terms of increased concentration and content of hydroxyproline in insoluble collagen and in total collagen and with a histological finding of fibrosis. These alterations were associated with a compromised systolic and diastolic LV function. Treatment with either an ARNI or captopril reduced systolic blood pressure (SBP), alleviated LV hypertrophy and fibrosis, and prevented the development of both systolic and diastolic LV dysfunction. Moreover, the serum levels of prolactin and prolactin receptor were reduced significantly by ARNI and slightly by captopril. In conclusion, in L-NAME-induced hypertension, the dual inhibition of neprilysin and AT1 receptors by ARNI reduced SBP and prevented the development of LV hypertrophy, fibrosis, and systolic and diastolic dysfunction. These data suggest that ARNI could provide protection against LV structural remodeling and functional disorders in hypertensive heart disease.
- Publikační typ
- časopisecké články MeSH
Hypertension-induced renal injury is characterized by structural kidney alterations and function deterioration. Therapeutics for kidney protection are limited, thus novel renoprotectives in hypertension are being continuously sought out. Ivabradine, an inhibitor of the If current in the sinoatrial node reducing heart rate (HR), was shown to be of benefit in various cardiovascular pathologies. Yet, data regarding potential renoprotection by ivabradine in hypertension are sparse. Thirty-six adult male Wistar rats were divided into non-diseased controls and rats with NG-nitro-L-arginine methyl ester (L-NAME)-induced hypertension to assess ivabradine's site-specific effect on kidney fibrosis. After 4 weeks of treatment, L-NAME increased the average systolic blood pressure (SBP) (by 27%), decreased glomerular density (by 28%) and increased glomerular tuft area (by 44%). Moreover, L-NAME induced glomerular, tubulointerstitial, and vascular/perivascular fibrosis by enhancing type I collagen volume (16-, 19- and 25-fold, respectively). L-NAME also increased the glomerular type IV collagen volume and the tubular injury score (3- and 8-fold, respectively). Ivabradine decreased average SBP and HR (by 8 and 12%, respectively), increased glomerular density (by 57%) and reduced glomerular tuft area (by 30%). Importantly, ivabradine decreased type I collagen volume at all three of the investigated sites (by 33, 38, and 72%, respectively) and enhanced vascular/perivascular type III collagen volume (by 67%). Furthermore, ivabradine decreased the glomerular type IV collagen volume and the tubular injury score (by 63 and 34%, respectively). We conclude that ivabradine attenuated the alterations of glomerular density and tuft area and modified renal fibrosis in a site-specific manner in L-NAME-hypertension. It is suggested that ivabradine may be renoprotective in hypertensive kidney disease.
- Publikační typ
- časopisecké články MeSH
AIMS: Our aims were to characterize human mesenchymal stem cells isolated from various tissues by pluripotent stem cells gene expression profile. METHODS: Four strains of dental pulp stem cells (DP-MSCs) were isolated from dental pulp tissue fragments adhered to plastic tissue culture dishes. Mesenchymal stem cells derived from umbilical cord tissue (UBC-MSCs) were isolated with the same technique. Bone marrow derived mesenchymal stem cells (BM-MSCs) were isolated from nucleated cells of bone marrow obtained by density gradient centrifugation. Human mesenchymal stem cells from adipose tissue (AT-MSCs) were isolated by collagenase digestion. All kinds of MSCs used in this study were cultivated in low glucose DMEM containing 5% or human platelet extract. All stem cell manipulation was performed in GMP conditions. Expression of 15 pluripotent stem cells genes on the level of proteins was measured by Proteome Profiler Human Pluripotent Stem Cell Array. Induction of MSCs to in vitro differentiation to adipocytes, osteoblasts, chondroblasts was achieved by cultivation of cells in appropriate differentiation medium. RESULTS: All MSCs tested were phenotypically similar and of fibroblastoid morphology. DP-MSCs and UBC-MSCs were more proliferative than bone marrow BM-MSCs and AT-MSCs. Protein expression of 15 genes typical for pluripotent stem cells distinguished them into two groups. While the gene expression profiles of BM-MSC, AT-MSCs and UBC-MSCs were similar, DP-MSCS differed in relative gene expression on the level of their products in several genes. CONCLUSIONS: Dental pulp mesenchymal stem cells cultivated in vitro under the same conditions as MSCs from bone marrow, adipose tissue and umbilical cord tissue can be distinguished by pluripotent stem cell gene expression profile.
- MeSH
- buněčná diferenciace fyziologie MeSH
- buněčné kultury MeSH
- buňky kostní dřeně cytologie MeSH
- exprese genu * MeSH
- fenotyp MeSH
- kultivované buňky MeSH
- lidé MeSH
- mezenchymální kmenové buňky cytologie MeSH
- proliferace buněk MeSH
- pupečník cytologie MeSH
- techniky in vitro MeSH
- tuková tkáň cytologie MeSH
- zubní dřeň cytologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
BACKGROUND: Diabetes mellitus is a metabolic disorder characterized by hyperglycemia. The abnormal glucose metabolism results from defects in insulin production or insulin action, or both. For decades, it was suspected that diabetes contributed to poorer oral health and the increased frequency of periodontitis. More recently it was found that periodontitis could adversely affect glycemic control in diabetics. This review focuses on the bidirectional relationship between diabetes mellitus and periodontitis. METHODS AND RESULTS: A review of the literature on periodontal disease in diabetes using the following key words: periodontitis/periodontal disease and diabetes mellitus, hyperglycemia/glycemic control. CONCLUSIONS: There is strong evidence for an association between diabetes mellitus and inflammatory periodontal disease. Diabetes mellitus increases the risk for and severity of periodontitis, and periodontal diseases can aggravate insulin resistance and affect glycemic control. Periodontal treatment improves glycemic control in type 2 diabetics; control of periodontal infection is not only important for oral health, it may also improve overall health.
- MeSH
- chronická parodontitida komplikace patofyziologie MeSH
- glykovaný hemoglobin metabolismus MeSH
- komplikace diabetu komplikace patofyziologie MeSH
- krevní glukóza metabolismus MeSH
- lidé MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
Cieľ práce: Zistiť výskyt fibróznych hyperplázií na maxile a mandibule na pracovisku. Materiál a metódy: Retrospektívna analýza súboru 124 pacientov (51 mužov, 73 žien, priemerný vek 45,7 rokov, rozmedzie 14–84 rokov) liečených v rokoch 2011 a 2012 s histologicky verifikovanými léziami: fokálna fibrózna hyperplázia (iritačný fibróm), epulis fibromatosa, gingivitis hyperplastica fibromatosa (medicamentosa, hereditaria alebo idiopathica) a granuloma fissuratum. Výsledky: Vyskytlo sa 12 prípadov fokálnej fibróznej hyperplázie (9,68 %), 29 epulis fibromatosa (23,39 %), 45 fibróznej hyperplastickej gingivitídy (36,29 %) a 38 epulis fissuratum (30,64 %). Medikamentóznu hyperplastickú gingivitídu spôsobovali u 40 pacientov antihypertenzíva blokujúce kalcium, v troch prípadoch imunosupresíva. Fisurálne granulómy prevládali u pacientov nosiacich snímateľné náhrady dlhšie ako päť rokov. Záver: Etiologický profil fibróznych hyperplázií sa v súčasnosti mení. Okrem vonkajších iritačných faktorov treba brať do úvahy aj individuálnu predispozíciu, zvlášť u idiopatických foriem.
Aim of Study: Evaluate prevalence of fibrous hyperplasias in maxilla and mandible at the workplace. Material and Methods: Retrospective analysis in a group of 124 patients (51 men, 73 women, mean age 45.7 years, range 14 – 84 years) treated within the years 2011 and 2012 with histologically verified lesions: focal fibrous hyperplasia (irritation fibroma), epulis fibromatosa, hyperplastic fibrous gingivitis (medicamentous, hereditary or idiopathic) and fissural granuloma. Results: 12 cases of focal fibrous hyperplasia (9.68 %), 29 epulis fibromatosa (23.39 %), 45 hyperplastic fibrous gingivitis (36.29 %) and 38 epulis fissuratum (30.64 %) were found there. Medicamentous hyperplastic gingivitis was caused in 40 patients by antihypertensive drugs blocking calcium, in three cases by immunosuppressors. Fissural granulomas prevailed in patients wearing removable dentures longer than five years. Conclusion: Aetiological profile of the fibrous hyperplasias changes recently. Besides the external irritation factors also individual predisposition should be considered, especially in idiopathic forms.
- Klíčová slova
- fibrózní hyperplazie,
- MeSH
- dospělí MeSH
- fibromatóza dásní epidemiologie MeSH
- gingivitida epidemiologie MeSH
- hyperplazie dásní * epidemiologie etiologie patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mandibula MeSH
- maxila MeSH
- mladiství MeSH
- mladý dospělý MeSH
- nemoci dásní epidemiologie MeSH
- retrospektivní studie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
Autori retrospektívne analyzovali súbor 174 pacientov (33 mužov, 141 žien) vyšetrených v rokoch 2004 – 2006 na Klinike stomatológie a maxilofaciálnej chirurgie v Bratislave pre suspektný primárny a sekundárny Sjögrenov syndrom, alebo sicca-like syndróm (vylúčení boli pacienti po liečbe orofaciálnych malignít). Biopsie malých slinných žlia z dolnej pery sa urobili u 156 (89,7 %) vyšetrených pacientov. Pri hodnotení fokus skóre nález dvoch a viac fokusov na 4 mm2 žľazového tkaniva potvrdil diagnózu Sjögrenovho syndrómu len u 27 (17,53 %) pacientov, nález jedného fokusu diagnózu podporil u 32 (20,78 %) pacientov. U 54 (35,06 %) pacientov sa zistil negatívny nález, alebo v 41 (26,63 %) iba nepatrné lymfocytárne infiltráty.Autori považujú za nesprávnu indikáciu biopsie malých slinných žliaz pred využitím ostatných diagnostických možností.Miesto odberu materiálu intraorálne z pery, resp. z líca, alebo extraorálne z príušnej žľazy neovplyvňuje podstatne výsledok, skôr treba zvážiť možné komplikácie. Biopsia slinných žliaz nesmie chýbať pri podozrení na primárny Sjögrenov syndróm.
Authors analysed retrospectively a group of 174 patients (33 men, 141 women) evaluated within years 2004-2006 at the Dept. of Stomatology and Maxillofacial Surgery in Bratislava for suspect primary and secondary Sjögren ́s syndrom or sicca-like syndrome (patients after treatment of oral cancer were excluded). Biopsies of minor salivary glands from the lower lip were performed in 156 (89.7 %) of cases. By the focus score evaluation finding of two or more focuses on 4 mm2 glandular tissue confirmed the diagnosis of the Sjögren’s syndrome only in 27 (17.53 %) of cases, finding of one focus supported the diagnosis in 32 (20.78 %) patients. Fifty four (35.06 %) of the patients had negative findings and in 41 (26.63 %) of the cases there appeared minimal lymphocytic infiltrates by the histopathology. The authors consider the indication of the biopsy before use of other diagnostic tools as incorrect. Site of biopsy intraorally (lip, buccal region) or extraorally (parotid gland) does not influence the result, but more frequent complications should be considered. Biopsy is obligatory by a suspect primary Sjögren ́s syndrome.
- MeSH
- biopsie využití MeSH
- diagnostické techniky endokrinologické využití MeSH
- lidé MeSH
- malé slinné žlázy patologie MeSH
- retrospektivní studie MeSH
- Sjögrenův syndrom diagnóza patofyziologie patologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- srovnávací studie MeSH
