Some mycobacterial species (particularly Mycobacterium marinum) found in aquarium environments may cause chronic diseases in fish and cutaneous infections in humans, the so-called 'fish tank granuloma'. The presence and distribution of mycobacterial species in clinically healthy aquarium fish and their environment has not been adequately explored. The present study analysed the occurrence of mycobacteria in a decorative aquarium (Brno, South Moravia) and in five aquaria of a professional fish breeder (Bohumin, North Moravia). After Ziehl-Neelsen staining, acid-fast rods (AFR) were observed in six (14.3%) and mycobacteria were detected by culture in 18 (42.9%) of 42 tissue samples from 19 fish. Sixty-five samples of the aqueous environment from all six aquaria were examined; AFR were found in 16 (24.6%) and mycobacteria were detected by culture in 49 (75.4%) samples. Forty-one (70.7%) of 58 selected mycobacterial isolates were identified biochemically as follows: M. fortuitum, M. flavescens, M. chelonae, M. gordonae, M. terrae, M. triviale, M. diernhoferi, M. celatum, M. kansasii and M. intracellulare. The clinically important species for humans and fish, M. marinum, was not detected. Mycobacterium kansasii was isolated from one sample of the aquarium environment from North Moravia, which is a region of the Czech Republic with endemic incidence of M. kansasii in water. The incidence of other conditionally pathogenic mycobacterial species in healthy fish and in all investigated constituents of the aquarium environment including snails and crustaceans used for fish feeding, was quite high. Accordingly, mycobacterial species from aquarium environments may serve as a possible source of infection for both aquarium fish and immunodeficient fish handlers.
- MeSH
- atypické mykobakteriální infekce epidemiologie veterinární MeSH
- ekosystém MeSH
- financování organizované MeSH
- incidence MeSH
- nemoci ryb epidemiologie mikrobiologie MeSH
- netuberkulózní mykobakterie izolace a purifikace klasifikace MeSH
- rybářství MeSH
- ryby MeSH
- životní prostředí MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
From Mycobacterium avium species Mycobacterium avium subsp. paratuberculosis (n=961), Mycobacterium a. avium (n=677), Mycobacterium a. silvaticum (n=5), and Mycobacterium a. hominissuis (n=1566) were examined, and from Mycobacterium tuberculosis complex M. tuberculosis (n=2), Mycobacterium bovis (n=13), M. bovis BCG (n=4), and Mycobacterium caprae (n=10) were examined. From other mycobacterial species Mycobacterium intracellulare (n=60) and atypical mycobacteria (n=256) including Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacterium scrofulaceum, Mycobacterium gastri and other species of conditionally pathogenic mycobacteria were analysed. The internal standard molecules corresponding to insertion sequences IS900, IS901, IS1245, and flanking region (FR300) of IS901 were produced by PCR of alfalfa genome segment and inserted into plasmid vector. The resulting recombinant plasmid molecules were used as internal standards in coamplification with a total of 4729 mycobacterial collection strains and field isolates between 1996 and 2003. The size differences between amplicons obtained from IS900 (258 bp), IS901 (1108 bp), IS1245 (427 bp), and FR300 (300 bp) and from corresponding internal standard molecules ISIS900 (591 bp), ISIS901 (1 336 bp), ISIS1245 (583 bp), and IS901 flanking region of 300 bp ISFR300 (488 bp), respectively, allowed easy discrimination. The internal amplicons were visible by naked aye on agarose gel when 10(1), 10(3), 10(2), and 10(2) molecules for ISIS900, ISIS901, ISIS1245, and ISFR300 were used in the PCR, respectively, when no bacterial DNA was added to the reaction. The system was tested to define the amount of internal standards that could be used in the PCR without affecting the amplification of the specific segment. Non-specific amplifications were observed in M. fortuitum with IS1245 PCR and mixed infections with M. a. avium and M. a. hominissuis from pigs and cattle were found. PCR results of typing were compared with serotyping and Accu-Probes analyses in selected field isolates.
- MeSH
- DNA bakterií genetika chemie MeSH
- financování organizované MeSH
- Mycobacterium genetika izolace a purifikace klasifikace MeSH
- mykobakteriózy diagnóza mikrobiologie veterinární MeSH
- nemoci prasat diagnóza mikrobiologie MeSH
- nemoci skotu diagnóza mikrobiologie MeSH
- polymerázová řetězová reakce metody veterinární MeSH
- prasata MeSH
- referenční standardy MeSH
- sérotypizace MeSH
- skot MeSH
- transpozibilní elementy DNA genetika MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
We investigated the presence of Mycobacterium avium subsp. paratuberculosis in retail cheeses from Greece and the Czech Republic. We found that 31.7% and 3.6% of our samples reacted positive by PCR and culture, respectively. Consumption of these cheeses is likely to result in human exposure to M. avium subsp. paratuberculosis, albeit at a low level for viable cells.
- MeSH
- DNA bakterií genetika izolace a purifikace MeSH
- DNA primery MeSH
- elektroforéza v agarovém gelu MeSH
- Mycobacterium avium subsp. paratuberculosis genetika izolace a purifikace MeSH
- Mycobacterium avium genetika izolace a purifikace MeSH
- polymerázová řetězová reakce MeSH
- sekvence nukleotidů MeSH
- sýr mikrobiologie MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Řecko MeSH
- MeSH
- finanční podpora výzkumu jako téma MeSH
- lidé MeSH
- mikrobiologické techniky MeSH
- mléčné výrobky MeSH
- mléko mikrobiologie MeSH
- Mycobacterium avium subsp. paratuberculosis patogenita MeSH
- Mycobacterium avium izolace a purifikace patogenita MeSH
- polymerázová řetězová reakce MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- Crohnova nemoc diagnóza patologie MeSH
- dospělí MeSH
- finanční podpora výzkumu jako téma MeSH
- intracelulární infekce bakterií Mycobacterium avium diagnóza patologie MeSH
- lidé MeSH
- mikrobiologické techniky metody MeSH
- Mycobacterium avium subsp. paratuberculosis patogenita MeSH
- paratuberkulóza diagnóza patologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
