Nanofibrous materials are used in drug delivery as carriers of active ingredients. These can be incorporated into the materials with various electrospinning methods that differ mainly in the way spinning solutions are prepared. Each method affects primarily the encapsulation efficiency and distribution of active ingredients in the materials. This study focuses on the incorporation of octenidine dihydrochloride (OCT) and triclosan (TRI) into nanofibrous materials electrospun from native hyaluronic acid emulsions, dispersions, and blends. OCT had no substantial effect on fiber morphology, which is affected by the solvent system. All OCT encapsulation efficiencies were comparable (approximately 90%). TRI encapsulation efficiencies varied greatly depending on the method used. Merely 3% of TRI was encapsulated when it was spun from a dispersion. Encapsulation efficiency was higher, and TRI was incorporated in clusters when an emulsion was used. The best result was achieved with a blend, in which case 96% of TRI was encapsulated.
- MeSH
- antiinfekční látky lokální chemie MeSH
- emulze chemie MeSH
- kyselina hyaluronová chemie MeSH
- nanovlákna chemie MeSH
- Publikační typ
- časopisecké články MeSH
Nanofibrous materials produced from natural polymers have wide range of potential uses in regenerative medicine. This paper focuses on preparation of nanofibrous layers produced from intentionally hydrophobized derivatives of hyaluronan, which is known for its ability to promote wound healing. This structural modification of hyaluronan expands the range of potential uses of this promising material, which is otherwise limited due to the hydrophilic nature of hyaluronic acid. The aim of this research was preparation of nanofibrous material that would retain its fibrous structure and dimensional stability even after getting into contact with an aqueous medium, which is impossible to achieve with layers composed solely of native hyaluronan. As a result, such material would be able to retain its breathability and good mechanical properties when both dry and wet. Furthermore, all prepared materials were proved non-toxic for cells. This self-supporting nanofibrous matrix can be used as a scaffold, or porous wound dressing.
- Publikační typ
- časopisecké články MeSH
Due to their large active surface, high loading efficiency, and tunable dissolution profiles, nanofibrous mats are often cited as promising drug carriers or antimicrobial membranes. Hyaluronic acid has outstanding biocompatibility, but it is hydrophilic. Nanofibrous structures made from hyaluronan dissolve immediately, making them unsuitable for controlled drug release and longer applications. We aimed to prepare a hyaluronan-based antimicrobial nanofibrous material, which would retain its integrity in aqueous environments. Self-supporting nanofibrous mats containing octenidine dihydrochloride or triclosan were produced by electrospinning from hydrophobized hyaluronan modified with a symmetric lauric acid anhydride. The nanofibrous mats required no cross-linking to be stable in PBS for 7 days. The encapsulation efficiency of antiseptics was nearly 100%. Minimal release of octenidine was observed, while up to 30% of triclosan was gradually released in 72 h. The nanofibrous materials exhibited antimicrobial activity, the fibroblast viability was directly dependent on the antiseptic content and its release.
- MeSH
- antibakteriální látky chemie farmakologie toxicita MeSH
- buňky 3T3 MeSH
- hydrofobní a hydrofilní interakce MeSH
- iminy chemie farmakologie toxicita MeSH
- kyselina hyaluronová chemie farmakologie toxicita MeSH
- léky s prodlouženým účinkem chemie farmakologie toxicita MeSH
- mikrobiální testy citlivosti MeSH
- myši MeSH
- nanovlákna chemie toxicita MeSH
- nosiče léků chemie farmakologie toxicita MeSH
- Pseudomonas aeruginosa účinky léků MeSH
- pyridiny chemie farmakologie toxicita MeSH
- Staphylococcus aureus účinky léků MeSH
- triclosan chemie farmakologie toxicita MeSH
- uvolňování léčiv MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Wound dressings with silver have been shown to be cytotoxic in vitro. However, the extrapolation of this cytotoxicity to clinical settings is unclear. We applied dressings with various forms of silver on porcine skin ex vivo and investigated silver penetration and DNA damage. We assessed antimicrobial efficacy, cytotoxicity to skin cells, and immune response induced by the dressings. All dressings elevated the DNA damage marker γ-H2AX and the expression of stress-related genes in explanted skin relative to control. This corresponded with the amount of silver in the skin. The dressings reduced viability, induced oxidative stress and DNA damage in skin cells, and induced the production of pro-inflammatory IL-6 by monocytes. The oxidative burst and viability of activated neutrophils decreased. The amount of silver released into the culture medium varied among the dressings and correlated with in vitro toxicity. However, antimicrobial efficiencies did not correlate strongly with the amount of silver released from the dressings. Antimicrobial efficiency and toxicity are driven by the form of silver and the construction of dressings and not only by the silver concentration. The damaging effects of silver dressings in ex vivo skin highlight the importance of thorough in vivo investigation of silver dressing toxicity.
- MeSH
- buněčné linie MeSH
- infekce v ráně MeSH
- kůže chemie cytologie účinky léků MeSH
- lidé MeSH
- obvazy škodlivé účinky MeSH
- poškození DNA * MeSH
- prasata MeSH
- stříbro toxicita MeSH
- techniky tkáňových kultur MeSH
- viabilita buněk účinky léků MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Cíl: Cílem práce je zhodnotit opacitu materiálu čočky AcryNovaTMPC 610Y z hydrofobního akrylátu a její vliv na zrakové funkce u pacientů podstupujících rutinní operaci katarakty 10 let po implantací nitrooční čočky. Metodika a soubor: Do studie bylo zařazeno 23 pacientů (26 očí) po operaci nekomplikovaného šedého zákalu s implantovanou čočkou AcryNovaTMPC 610Y mezi lety 2005–2007. Pacienti byli vyšetřeni v prvním pooperačním týdnu a stav oka zkontrolován 10 let po operaci. Hodnotili jsme nejlépe korigovanou zrakovou ostrost (NKZO), kontrastní citlivost jsme vyšetřovali při kontrole v mezopických podmínkách na tabulích CSV–1000E, stupeň opacity nitrooční čočky (NOČ) a výskyt opacit zadního pouzdra (PCO–Posterior Capsule Opacification) pomocí Scheimpflugovy kamery Pentacamu (OCULUS Pentacam HR). Explantovanou čočku jsme vyšetřili pomocí předně segmentového OCT (Optovue, Avanti RTVue XR) a spektroskopickou metodou EDX (Energy Dispersive X-Ray Spectroscopy).Výsledky: Postoperační NKZO při prvním vyšetření byla 0,95 ± 0,10, po deseti letech jsme zaznamenali signifikantní zhoršení NKZO v souboru na 0,87 ± 0,20. Průměrná opacita materiálu NOČ měřená postoperačně byla 6,37 ± 2,16 %, při kontrole po deseti letech stoupla opacita v průměru na 14,22 ± 5,87 %. I když jsme zaznamenali statisticky signifikantní pokles NKZO v souboru, nejvíce byla ze zrakových funkcí postižena kontrastní citlivost. Po deseti letech bylo 50 % pacientů bez jakékoliv opacity zadního pouzdra. U explantované NOČ jsme pomocí laboratorních vyšetřovacích postupů prokázali optické změny materiálu ve smyslu snížení jeho transparence a změny struktury vedoucí k jeho hydrofilitě. Závěr: Po deseti letech od implantace došlo k signifikantnímu zvýšení opacity materiálu NOČ z hydrofobního akrylátu, která vede ke snížení kontrastní citlivosti a posléze i k poklesu zrakové ostrosti. Změny se týkají jen části vyšetřených čoček potažmo čoček se stejnými šaržemi. To vede k závěru, že tyto čočky byly primárně vyrobeny z nekvalitní suroviny.
Purpose: To report late postoperative opacification of a hydrophobic acrylic intraocular lens (IOL) AcryNovaTMPC 610Y as well as the clinical consequences in patients 10 years after uncomplicated cataract surgery. Materials and Methods: Medical records were reviewed of 23 patients (26 eyes) with AcryNovaTMPC 610Y implantated between years 2005 and 2007. Next clinical examination was performed 10 years after surgery. We assessed best corrected distance visual acuity (BCDVA), contrast sensitivity (CSV-1000E) and relative opacity of IOL material, (OCULUS Pentacam HR). Results of BCDVA and Pentacam were analysed statistically. One explanted IOL was analysed using anterior segment OCT in vitro and spectroscopic method EDX (Energy-dispersive X-ray spectroscopy).Results: Opacification led to a statistically significant reduction in the best corrected distance visual acuity (BCDVA) = (0,95 ± 0,10) versus (0,87 ± 0,20) and o increase of IOL opacity only in some lenses but statistically significant in the average (6,37 ± 2,16)% versus value of (14,22 ± 5,87)%. In the explanted IOL we have documented structural changes of primarily hydrophobic raw material leading to property of hydrophilic one. Conclusion: Some batches of AcryNovaTMPC 610Y were produced from raw material of poor quality which is the cause of its structural changes and its progressive opacification.
In this work, a hybrid copolymer consisting of poly(3-hydroxybutyrate) grafted to hyaluronic acid (HA) was synthesised and characterised. Once formed, the P(3HB)-g-HA copolymer was soluble in water allowing a green electrospinning process. The diameters of nanofibres can be tailored by simply varying the Mw of polymer. The optimization of the process allowed to produce fibres of average diameter in the range of 100-150 nm and low polydispersity. The hydrophobic modification has not only increased the fibre diameter, but also the obtained layers were homogenous. At the nanoscale, the hybrid copolymer exhibited an unusual hairy topography. Moreover, the hardness and tensile properties of the hybrid were found to be superior compared to fibres made of unmodified HA. Particularly, this reinforcement was achieved at the longitudinal direction. Additionally, this work reports the use in the composition of a water-soluble copolymer containing photo cross-linkable moieties to produce insoluble materials post-electrospinning. The derivatives as well as their nanofibrous mats retain the biocompatibility of the natural polymers used for the fabrication.
- MeSH
- biokompatibilní materiály * chemická syntéza chemie MeSH
- biomechanika MeSH
- hydrofobní a hydrofilní interakce MeSH
- hydroxybutyráty chemická syntéza chemie MeSH
- kyselina hyaluronová chemie MeSH
- nanovlákna chemie MeSH
- polyestery chemická syntéza chemie MeSH
- polymery chemická syntéza chemie MeSH
- poskytování zdravotní péče MeSH
- tkáňové podpůrné struktury chemie MeSH
- vstřebatelné implantáty * MeSH
- zdravotnické prostředky MeSH
- Publikační typ
- časopisecké články MeSH
