The aim of this study was to investigate the use of a standardized animal model subjected to antibiotic treatment, and the effects of this treatment on the course of dextran sodium sulphate (DSS)-induced colitis in mice. By decontamination with selective antibiotics and observation of pathogenesis of ulcerative colitis (UC) induced chemically by exposure of mice to various concentrations of DSS, we obtained an optimum animal PGF model of acute UC manifested by mucin depletion, epithelial degeneration and necrosis, leading to the disappearance of epithelial cells, infiltration of lamina propria and submucosa with neutrophils, cryptitis, and accompanied by decreased viability of intestinal microbiota, loss of body weight, dehydration, moderate rectal bleeding, and a decrease in the selected markers of cellular proliferation and apoptosis. The obtained PGF model did not exhibit changes that could contribute to inflammation by means of alteration of the metabolic status and the induced dysbiosis did not serve as a bearer of pathogenic microorganisms participating in development of ulcerative colitis. The inflammatory process was induced particularly by exposure to DSS and its toxic action on compactness and integrity of mucosal barrier in the large intestine. This offers new possibilities of the use of this animal model in studies with or without participation of pathogenic microbiota in IBD pathogenesis.
- MeSH
- antibakteriální látky farmakologie MeSH
- apoptóza fyziologie MeSH
- epitelové buňky patologie MeSH
- modely nemocí na zvířatech MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- proliferace buněk fyziologie MeSH
- síran dextranu farmakologie MeSH
- střevní mikroflóra účinky léků fyziologie MeSH
- střevní sliznice mikrobiologie patologie MeSH
- ulcerózní kolitida chemicky indukované farmakoterapie patologie MeSH
- zánět farmakoterapie patologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- ateroskleróza * etiologie chemicky indukované MeSH
- cholesterol krev škodlivé účinky MeSH
- dieta s vysokým obsahem tuků MeSH
- kolorektální nádory * etiologie chemicky indukované MeSH
- kyseliny mastné omega-6 škodlivé účinky MeSH
- lipidy krev škodlivé účinky MeSH
- mastné kyseliny krev škodlivé účinky MeSH
- metabolismus lipidů * MeSH
- modely nemocí na zvířatech * MeSH
- omega-3 mastné kyseliny krev škodlivé účinky MeSH
- potkani Sprague-Dawley MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
BACKGROUND/AIM: Chemopreventive activity of a new probiotic strain Lactobacillus plantarum LS/07 (PRO) and prebiotic oligofructose-enriched inulin (PRE) in rat mammary carcinogenesis induced by procarcinogen 7,12-dimethylbenz[a]anthracene has been reported before. This study evaluated the anticancer and immunomodulatory efficacy of PRO, PRE, PRO+PRE (PRO/PRE) and combination with melatonin (PRO+PRE+MEL) in a rat model, when breast cancer was induced by a direct-acting carcinogen N-nitroso-N-methylurea (NMU). MATERIALS AND METHODS: Daily administration of PRO (at a dose of 8.4×10(8) colony-forming units (c.f.u.)/rat), PRE (in the diet, 20 g/kg) and MEL (in tap water, 20 mg/l) started 14 days before the first NMU dose and lasted for 16 weeks. RESULTS: Although tumor growth was not altered, a marked decrease in the ratio of high-/low-grade carcinomas and in tumoral Ki-67 expression was found after PRO+PRE treatment; melatonin augmented these effects. PRO+PRE+MEL combination enhanced CD4(+) and CD8(+) T-cell tumor infiltration induced by PRO/PRE and increased CD25(+)FoxP3(+) regulatory T-cells in tumors. CONCLUSION: In mammary carcinogenesis, Lactobacillus plantarum LS/07 and inulin exert prodifferentiating, antiproliferative and immunomodulatory activities, which are significantly amplified by melatonin co-administration.
- MeSH
- experimentální nádory mléčných žláz farmakoterapie MeSH
- imunologické faktory farmakologie MeSH
- interleukin-6 fyziologie MeSH
- inulin farmakologie MeSH
- krysa rodu rattus MeSH
- Lactobacillus plantarum * MeSH
- melatonin farmakologie MeSH
- methylnitrosomočovina MeSH
- potkani Sprague-Dawley MeSH
- probiotika farmakologie MeSH
- protinádorové látky farmakologie MeSH
- transformující růstový faktor beta1 fyziologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The aim of our study was to evaluate the effects of the different probiotic strains, Lactobacillus plantarum LS/07 and Lactobacillus plantarum Biocenol LP96, on lipid metabolism and body weight in rats fed a high fat diet. Compared with the high fat diet group, the results showed that Lactobacillus plantarum LS/07 reduced serum cholesterol and LDL cholesterol, but Lactobacillus plantarum Biocenol LP96 decreased triglycerides and VLDL, while there was no change in the serum HDL level and liver lipids. Both probiotic strains lowered total bile acids in serum. Our strains have no significant change in body weight, gain weight, and body fat. These findings indicate that the effect of lactobacilli on lipid metabolism may differ among strains and that the Lactobacillus plantarum LS/07 and Lactobacillus plantarum Biocenol LP96 can be used to improve lipid profile and can contribute to a healthier bowel microbial balance.
- MeSH
- dieta s vysokým obsahem tuků * MeSH
- glukuronidasa metabolismus MeSH
- krysa rodu rattus MeSH
- Lactobacillus plantarum enzymologie metabolismus MeSH
- lipidy krev MeSH
- metabolismus lipidů * MeSH
- probiotika aplikace a dávkování metabolismus MeSH
- tělesná hmotnost MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Úvod: Fibrilácia predsiení (FP) je jednou z najčastejších komplikácií po operácii srdca. Cieľom práce bolo overiť, či existuje vzťah medzi markermi zápalu, oxidačným stresom a pooperačnou arytmiou. Metodika: 45 pacientov s ischemickou chorobou srdca (12 žien a 33 mužov, priemerný vek 62,3 ? 9,4 rokov) podstúpilo chirurgickú revaskularizáciu myokardu. Peroperačne bol mimotelový obeh (ECC) použitý u 30 pacientov, bez mimotelového obehu bolo revaskularizovaných 15 pacientov. V období prvých 3 pooperačných dní bol stanovený výskyt a dĺžka trvania FP, laboratórne markery zápalu (CRP, leukocyty, TNFα), marker oxidačného stresu (malóndialdehyd – MDA). Výsledky: Demografické údaje a pridružené ochorenia boli u týchto pacientov podobné. Vznik FP sme dokumentovali u 30 pacientov (66,7 %). U pacientov s pooperačnou FP boli významne vyššie hladiny zápalových parametrov (leukocyty 13,6 ? 3,6 vs 11,3 ? 3,6; 14,7 ? 3,9 vs 12,5 ? 2,9; 13,7 ? 4,1 vs 11,4 ? 13,7; p ≤ 0,05; CRP 138,1 ? 41,1 vs 69,9 ? 25,8; p ≤ 0,001; TNFα 11,3 ? 14,3 vs 8,7 ? 3,6; 12,1 ? 14,5 vs 8,7 ? 3,1; p ≤ 0,05) v porovnaní s pacientmi, u ktorých sa FP nevyskytla. Hodnoty MDA sa signifikantne nelíšili. Záver: Pacienti s pooperačne prítomnou fibriláciou predsiení mali vyššie hladiny zápalových markerov v porovnaní s pacientmi so sínusovým rytmom, avšak nevýznamné rozdiely v hladinách markeru oxidačného stresu.
Introduction: Atrial fibrillation (AF) is one of the most common complications following heart surgery. The aim of this work was to verify the relationship between inflammatory markers, oxidative stress and postoperative arrhythmia. Methods: 45 patients with ischemic heart disease (12 women and 33 men, mean age 62.3 ? 9.4 years) underwent surgical myocardial revascularization. The extracorporeal circulation (ECC) was used in 30 patients, without ECC was 15 patients. During the first 3 postoperative days was determining the incidence and duration of the AF, laboratory markers of inflammation (CRP, leukocytes, TNFα), malondialdehyde (MDA). Results: Demographic data and associated disease were in this patients similar. The incidence of AF we documented in 30 patients (66.7%). In patients with postoperative AF were significantly higher levels of inflammatory markers (leukocytes 13.6 ? 3.6 vs 11.3 ? 3.6; 14.7 ? 3.9 vs 12.5 ? 2.9; 13.7 ? 4.1 vs 11.4 ? 13.7; p ≤ 0.05; CRP 138.1 ? 41.1 vs 69.9 ? 25.8; p ≤ 0.001; TNFα 11.3 ? 14.3 vs 8.7 ? 3.6; 12.1 ? 14.5 vs 8.7 ? 3.1; p ≤ 0.05) compared with patients who were free from AF. Values of MDA were not significantly different. Conclusion: Patients with postoperative atrial fibrillation were higher levels of inflammatory markers compared with patients with sinus rhythm but no significant differences in the levels of oxidative stress.
- MeSH
- biologické markery analýza krev MeSH
- C-reaktivní protein * analýza MeSH
- fibrilace síní * epidemiologie etiologie MeSH
- lidé MeSH
- malondialdehyd * diagnostické užití MeSH
- mimotělní oběh * škodlivé účinky využití MeSH
- oxidační stres * fyziologie MeSH
- počet leukocytů * MeSH
- pooperační komplikace * epidemiologie etiologie MeSH
- revaskularizace myokardu * metody škodlivé účinky MeSH
- TNF-alfa * diagnostické užití MeSH
- zánět * krev MeSH
- Check Tag
- lidé MeSH
- MeSH
- Bacteria MeSH
- lidé MeSH
- probiotika farmakologie terapeutické užití MeSH
- Check Tag
- lidé MeSH
