Five types of amide-amine Carbon Nano-Particles (CNPs) were prepared by functionalization of CNPs and characterized by several analytical methods. The successful grafting of amines on CNPs was verified by X-ray photoelectron spectroscopy (XPS), organic elemental analysis and electrokinetic analysis. The size and morphology of CNPs were determined from transmission electron microscopy. The surface area and porosity of CNPs were examined by adsorption and desorption isotherms. Differential scanning calorimetry was used to investigate thermal stability of CNPs. The amount of bonded amine depends on its dimensionality arrangement. Surface area and pore volumes of CNPs decrease several times after individual amino-compound grafting. Selected types of functionalized CNPs were grafted onto a plasma activated surface of HDPE. The successful grafting of CNPs on the polymer surface was verified by XPS. Wettability was determined by contact angle measurements. Surface morphology and roughness were studied by atomic force microscopy. A dramatic decrease of contact angle and surface morphology was observed on CNP grafted polymer surface. Cytocompatibility of modified surfaces was studied in vitro, by determination of adhesion, proliferation and viability of vascular smooth muscle cells (VSMCs). Grafting of CNPs onto the polymer surface has a positive effect on the adhesion, proliferation and viability of VSMCs.
- MeSH
- aminy chemie MeSH
- kultivované buňky MeSH
- lidé MeSH
- myocyty hladké svaloviny cytologie účinky léků MeSH
- nanočástice škodlivé účinky chemie MeSH
- polyethylen chemie MeSH
- proliferace buněk účinky léků MeSH
- smáčivost MeSH
- svaly hladké cévní cytologie MeSH
- uhlík chemie MeSH
- viabilita buněk účinky léků MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Běžnou praxí v případě alergických reakcí na léky je medikament vysadit a nahradit jej lékem z jiné lékové skupiny. Indukce lékové tolerance (ILT) je další léčebnou možností, kdy pacient s alergií na lék je schopen jej tolerovat bez vedlejších nežádoucích účinků.
- MeSH
- Crohnova nemoc farmakoterapie MeSH
- desenzibilizace imunologická * MeSH
- lidé středního věku MeSH
- lidé MeSH
- monoklonální protilátky * imunologie terapeutické užití MeSH
- ošetřovatelská péče MeSH
- péče o pacienta MeSH
- tolerance léku * imunologie MeSH
- výsledek terapie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
Epidemics spread many types of pathogenic bacterial strains, especially strains of MRSA (Methicillin-resistant Staphylococcus aureus), which are being increasingly reported in many geographical areas [1]. This is becoming to be a serious global problem, particularly in hospitals. Not only are antibiotics proving to be increasingly ineffective but also the bacteria responsible for more than 70% of hospital-acquired bacterial infections are resistant to at least one of the drugs commonly used to treat them. In this study, hybrid coating A1 and nanocomposite hybrid coating A2 based on TMSPM (3-(trimethoxysilyl)propyl methacrylate, MMA (methyl methacrylate), TEOS (tetraethyl orthosilicate) and IPTI (titanium isopropoxide) containing silver and copper ions with or without nanoparticles of titanium dioxide were prepared by the sol-gel method. They were deposited on glass, poly(methyl methacrylate) and cotton using dip-coating or spin-coating, and then cured at 150 °C for 3 h or, in the case of poly(methyl methacrylate), at 100 °C for 4.5 h. The morphology and microstructure of these hybrid coatings were examined by SEM. The abrasion resistance was tested using a washability tester and found to depend heavily on the curing temperature. Seven types of bacterial strains were used to determine the profile of antibacterial activity, namely Escherichia coli, Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus - MRSA (CCM 4223), MRSA-2 (CCM 7112), Acinetobacter baumanii, Pseudomonas aeruginosa, and Proteus vulgaris (according to ALE-G18, CSNI). All the samples were tested by irradiating with either a UV-A or a daylight fluorescent lamp. All types of hybrid coating A1 and nanocomposite hybrid coating A2 were found to possess an excellent antibacterial effect, including against the pathogenic bacterial strains of MRSA, which present a dangerous threat on a global scale.
- MeSH
- Acinetobacter baumannii účinky léků účinky záření MeSH
- antibakteriální látky chemie farmakologie MeSH
- biokompatibilní potahované materiály chemie farmakologie MeSH
- měď chemie MeSH
- methicilin rezistentní Staphylococcus aureus účinky léků účinky záření MeSH
- methylmetakrylát chemie MeSH
- nanokompozity chemie MeSH
- organokovové sloučeniny chemie MeSH
- Proteus vulgaris účinky léků účinky záření MeSH
- Pseudomonas aeruginosa účinky léků účinky záření MeSH
- silany chemie MeSH
- stříbro chemie MeSH
- světlo MeSH
- ultrafialové záření MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
