The objective of the study was to determine the effects of inferior alveolar nerve transection on inorganic components in mandibular molars of the rat. We used 26 male laboratory rats of the Wistar strain for the study, age 7-9 weeks. The rats were divided in three groups. The control group (intact) included 6 rats. The surgery was performed under general anesthesia. The experimental group included (group with the nerve transected on the left) included 12 rats. The sham group (group with the nerve prepared without transection) included 8 rats. The animals were sacrificed after 4 weeks. Molars from the left and right sides of the mandible were extracted. Element content levels were determined using inductively coupled plasma mass spectrometry. The following elements were determined in all samples: magnesium (Mg), sodium (Na), potassium (K), calcium (Ca), zinc (Zn), and strontium (Sr). The nerve transection caused: a reduction of the contents of Ca and Sr in the mandibular molars; an increase in the contents of Mg and Zn; a difference arrangement of both sides for Na. The surgery approach itself caused a decrease in the contents of Na and K in the experimental and sham groups; the difference in K in M3 between the left and right sides disappeared due to the surgery. Our results have confirmed the hypothesis of inferior alveolar nerve transection having an effect on inorganic components in mandibular molars in the rat.
- MeSH
- krysa rodu rattus MeSH
- mandibula * chirurgie MeSH
- moláry chirurgie MeSH
- nervus mandibularis * fyziologie chirurgie MeSH
- potkani Wistar MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
OBJECTIVE: The aim of the study was to test the effect of transecting the inferior alveolar nerve on the inorganic bone component of the rat mandible. METHODS: 7-9 weeks old, male Wistar rats were used for the study. The animals were divided in 3 groups: control, experimental (nerve was transected) and sham (nerve was only prepared but not transected). After 4 weeks, the animals were killed, their teeth were extracted, and the mandibular bone was divided in 4 parts. Inductively coupled plasma mass spectrometry was used to the levels of 7 elements in the bone. RESULTS: The study results demonstrate that transection of the inferior alveolar nerve caused a decrease in calcium, iron, and strontium, and an increase of zinc. It caused the differences in potassium contents between the sides was significantly lower in the experimental group. The increase in the magnesium content, and decrease of sodium and potassium in the experimental group, as well as differences in the contents of: magnesium, sodium, potassium, iron and zinc between individual locations in the mandible are associated with the surgical approach. CONCLUSION: The results support our hypothesis - that sensory innervation has an impact on the inorganic component of the mandibular bone.
Cílem studie bylo testovat vliv přerušení nervus alveolaris inferiorna vybrané stopové prvky kosti dolní čelisti potkana. Materiál a metodika. Pro studii jsme použili 7–9 týdnů staré samce laboratorních potkanů kmene Wistar. Zvířata jsme rozdělili do tří skupin: kontrolní skupina (intaktní), experimentální skupina (s přerušeným nervem na levé straně) a ,,sham“ skupina (nerv jsme pouze vypreparovali, ale nepřerušili). Po 4 týdnech jsme zvířata usmrtili a extrahovali zuby (nebyly součástí studie). Kost dolní čelisti jsme rozdělili na 4 části. Ke stanovení 7 prvků v kosti jsme použili hmotnostní spektrometrii s indukčně vázaným plazmatem. Výsledky. Ze studie vyplývá, že přerušení nervu způsobilo snížení obsahu kobaltu, niklu a baria. Dále bylo příčinou vymizení rozdílu obsahů rubidia mezi levou a pravou stranou čelisti. Chirurgický přístup měl za následek změnu distribuce obsahů rubidia, molybdenu a baria v dolní čelisti a vymizení stranového rozdílu u baria v hřebeni a u niklu v meziálním místě a v hřebeni. U manganu a mědi jsme neprokázali zásadní rozdíly. Popsané změny nejspíše souvisejí s určitou přestavbou spongiózní kosti a s jejím úbytkem, jak zaznamenali jiní. Závěr. Výsledky podporují naši hypotézu, že senzitivní inervace ovlivňuje stopové prvky v kosti dolní čelisti potkana.
Objective. The purpose of the study was to test the effect of transection the inferior alveolar nerve on selected trace elements in the mandibular bone of the rat.Material and methodsWe used 7–9 weeks old males of Wistar laboratory rats for the study. The animals were divided in three groups: control (intact) group, experimental group (with the nerve transected on the left) and the sham group (the nerve was only exposed, not transected). The animals were sacrificed 4 weeks later and their teeth were extracted (not part of the study). The mandibular bone was divided in 4parts. Inductively coupled plasma mass spectrometry was used to determine 7 elements in the bone.Results. As follows from the study, nerve transection resulted in decreased contents of cobalt, nickel and barium. Furthermore, it resulted in disappearing of rubidium content differences on the left and right side of the mandible. The surgical approach itself causes changes in the distribution of rubidium, molybdenum and barium contents of the mandible and disappearance of the left and right side difference for barium in the ridge and for nickel at the mesial location and in the ridge. No sub-stantial differences were observed for manganese and copper. The described changes are probably related to acertain remodelling of the cancellous bone and its loss, as noted by others. Conclusion. The results support our hypothesis stating that sensory innervation affects trace elements in the mandibular bone.
Objective. Experimental lesions in the inferior alveolar nerve (IAN) are used for the study of peripheral and central alterations. The objective of our study was to contribute to a more precise description of the approach to the IAN and creating a lesion. Material and methods. Twenty-six males of Wistar laboratory rats were used for the study. The animals were divided into three groups: control group (6 rats), experimental group (12 rats - a part of the bone above the mandibular canal was removed under general anaesthesia using extraoral approach, after exposing a part of the IAN, the nerve was excised in a length of 3 mm), and a sham group (8 rats - the nerve was only dissected but not transected). Persisting denervation was verified using surgical revision and histological and immunohistochemical analysis after the observation period (4 weeks). Results. No evidence of re-innervation after 4 weeks. We found no statistically significant differences in mean weight gains between individual groups during the observation period. Conclusion. The described technique used in the study is one of the possible ways to create a nerve lesion at the site of the main trunk of the nerve. At the same time, the study provides a more precise description of the anatomical situation and approach to the IAN in the mandibular canal.
Eosinophilic angiocentric fibrosis (EAF) is a rare benign lesion of unknown aetiology, found predominantly in the upper respiratory tract. The fibrosis must be confirmed by considering other lesions in the differential diagnosis. The typical histological characteristics are the basis for establishing the diagnosis. We present a patient with long-term nasal obstruction caused by EAF. The patient had repeated resections of the lesion for its recurrences. As follows from the literature, radical resection is crucial for the treatment of EAF. This procedure may be difficult at times given the extent and localization of the disease.
Intraoseální hemangiom je benigní léze, která představuje méně než 1 % vše kostních nádorů. V oblasti lícní kosti se vyskytuje vzácně. Častější je výskyt u žen než u mužů. V článku prezentujeme případ pacientky s intraoseálním kavernózním hemagiomem lícní kosti. Tumor byl příčinou deformace lícní kosti vlevo. Léze byla patrná na výpočetní tomografii (CT) a na již dříve provedené magnetické rezonanci (MRI) pro obtíže nesouvisející s vyšetřovanou lézí. Provedli jsme kompletní odstranění tumoru včetně okraje zdravé kosti. Defekt jsme překlenuli titanovou minidlahou. Stav pět měsíců po operaci ukázal dobrý kosmetický efekt. Kontrolní CT a MRI vyšetření neprokázaly recidivu ani reziduum tumoru. Zároveň uvádíme přehled dostupné literatury.
Intraosseous haemangioma is a benign lesion that accounts for less than 1% of all bone tumours. It is found in the zygoma only rarely. This type of tumour is more common in women than in men. The paper presents the case of a female patient with intraosseous cavernous haemangioma in the zygoma. The tumour caused a deformation of the zygoma on the left. The lesion was visible in computed tomography (CT) scans, and also in magnetic resonance imaging (MRI) scans obtained previously due to problems unrelated to the lesion. We performed complete removal of the tumour including edges of healthy bone. The defect was bridge using a titanium miniplate. A good cosmetic effect was seen after 5 months from the surgery. Follow up CT and MRI evaluation showed no recurrence or residue of the tumour. The paper also includes a review of available literature.
- MeSH
- dospělí MeSH
- kavernózní hemangiom * diagnostické zobrazování chirurgie MeSH
- kostní destičky MeSH
- lícní kost * MeSH
- lidé MeSH
- magnetická rezonanční tomografie metody MeSH
- nádory kostí * diagnostické zobrazování chirurgie MeSH
- počítačová rentgenová tomografie metody MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
The original article was published in Folia Biologica (Praha) Volume 64, No. 3 (2018), 84-96.
- Publikační typ
- tisková chyba MeSH
The effect of the nervous system on bone remodelling has been described by many studies. Sensory and autonomic nerves are present in the bone. Immunohistochemical analysis of the bone have indicated the presence of neuropeptides and neurotransmitters that act on bone cells through receptors. Besides carrying sensory information, sensory neurons produce various neuropeptides playing an important role in maintaining bone and tooth pulp homeostasis, and dentin formation. Bone tissue and teeth contain organic and inorganic components. Bone cells enable bone mineralization and ensure its formation and resorption. Studies focused on the effects of the nervous system on the bone are proceeded using various ways. Sensory denervation itself can be achieved using capsaicin causing chemical lesion to the nerve. Surgical ways of causing only sensory lesion to nerves are substantially limited because many peripheral nerves are mixed and contain a motor component as well. From this point of view, the experimental model with transection of inferior alveolar nerve is appropriate. This nerve provides sensory innervation of the bone and teeth of the mandible. The purpose of our paper is to provide an overview of the effects exerted by the nervous system on the inorganic component of the bone and teeth, and also to present an overview of the used experimental models. As we assume, the transection of inferior alveolar nerve could be reflected in changed contents and distribution of chemical elements in the bone and teeth of rat mandible. This issue has not been studied so far.
- MeSH
- denervace MeSH
- krysa rodu rattus MeSH
- neurony aferentní MeSH
- zubní dřeň * inervace MeSH
- zuby * inervace MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
The purpose of the study was to test the hypothesis of different distribution spaces of elements in the rat mandibular bone and teeth. We used six adult males of Wistar laboratory rats for the study. After killing the animals, we extracted the molars and removed incisor crowns. The mandibular bone was divided into four parts (mesial-central-distal-ridge). Inductively coupled plasma mass spectrometry was used to determine the presence of 41 elements in the bone and tooth. Evidence of 14 elements was found in all samples (incisors-molarsbone). Generally, significant differences between the left and right side were found for K and Rb in the bone locations. As regards statistically significant differences in incisors-molars-bone locations, the elements for which these differences were found for all comparisons are listed as incisors versus individual molars, incisors versus bone locations, and individual molars versus bone locations: a) incisors-molars: Ba, Mn, Mo, Sr, Zn, K, Mg and Rb; b) incisors-bone: Fe, K, Mg, Mn, Na, Zn and Ba; c) molars-bone: Mn, Mo, Na and Mg. Statistically significant differences were also found between molars for Fe, Mg, Mn, and Sr and between bone locations for Ba, Ca, Mn, Sr, K, Rb, Zn, Mo, Mg, and Na. The elements Cu, Ni and Co were without pronounced differences. Twenty-seven elements were below the detection limit. Our results indicate different distributions of some elements in the rat mandibular incisors-molars-bone. We assume that the knowledge of chemical element contents in the laboratory rat bone and teeth will prove useful in experimental research of both these hard tissues.
- MeSH
- analýza rozptylu MeSH
- mandibula metabolismus MeSH
- potkani Wistar MeSH
- prvky * MeSH
- zuby metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Publikační typ
- abstrakt z konference MeSH
