Histopathological analysis can provide important information in long-term experiments with total artificial heart (TAH). Recently, a new type of blood pump, the helical flow total artificial heart (HF-TAH) was developed. This study aimed to investigate the changes in selected vital organs in animal experiments with implanted HF-TAH. Samples from lung, liver, and kidneys from two female goats (No. 1301 and No. 1304) with implanted HF-TAH were analyzed. Tissue samples were fixed in 10% formaldehyde and 4 µm thick transverse sections were stained with hematoxylin-eosin (HE). Additional staining was done for detection of connective tissue (Masson-Goldner stain) and for detection of iron (hemosiderin) deposits (Perls stain). Sections were scanned at 100× and 500× magnification with a light microscope. Experiment no. 1301 survived 100 days (cause of termination was heavy damage of the right pump); experimental goat no.1304 survived 68 days and was sacrificed due to severe right hydrodynamic bearing malfunction. Histopathological analysis of liver samples proved signs of chronic venostasis with limited focal necrotic zones. Dilated tubules, proteinaceous material in tubular lumen, and hemosiderin deposits were detected in kidney samples. Contamination of the organs by embolized micro-particles was suspected at the autopsy after discovery of visible damage (scratches) of the pump impeller surface (made from titanium alloy) in both experiments. Sporadic deposits of foreign micro-particles (presumably titanium) were observed in most of the analyzed parenchymal organs. However, the described deposits were not in direct connection with inflammatory reactions in the analyzed tissues. Histopathological analysis showed the presence of minimal contamination of the lung, kidney, and liver tissue samples by foreign material (titanium very likely). The analysis showed only limited pathological changes, especially in liver and kidneys, which might be attributed to the influence of artificial perfusion often observed in chronic TAH experiments.

• MeSH
• implantace protézy škodlivé účinky   MeSH
• játra patologie   MeSH
• kozy MeSH
• ledviny patologie   MeSH
• plíce patologie   MeSH
• protézy - design MeSH
• titan analýza   MeSH
• umělé srdce * škodlivé účinky   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• biomedicínský výzkum dějiny   MeSH
• dějiny 20. století MeSH
• dějiny 21. století MeSH
• interinstitucionální vztahy MeSH
• kardiologie dějiny   MeSH
• kooperační chování MeSH
• lidé MeSH
• podpůrné srdeční systémy dějiny   MeSH
• protézy - design MeSH
• umělé srdce dějiny   MeSH
• zvířata MeSH
• Check Tag
• dějiny 20. století MeSH
• dějiny 21. století MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• historické články MeSH
• úvodníky MeSH
• Geografické názvy
• Česká republika MeSH

Hemodialyzed (HD) patients with end-stage renal disease (ESRD) exhibit lower fitness as a consequence of chronic uremic changes that trigger various structural, metabolic, and functional abnormalities in skeletal muscles. The aim of this randomized study was to compare the effect of rehabilitation (RHB) training on a bicycle ergometer and electromyostimulation (EMS) of leg extensors in HD patients with ESRD. Thirty-two HD patients (18 men/14 women; mean age 61.1 ± 8.8 years) were randomized into three groups: (i) exercise training (ET; n = 11) on bicycle ergometer 2 × 20 min; (ii) EMS (n = 11) where stimulation (10 Hz) of leg extensors was applied for 60 min; and (iii) controls (CON; n = 10) without exercise. Exercising was performed between the 2nd and the 3rd hour of HD, three times a week, 20 weeks in total. Ergometric test was performed in order to evaluate peak workload (W(peak)), 6-min corridor walking test (CWT) to evaluate the distance walked, and dynamometry of leg extensors to assess muscle power (F(max)). Urea clearance was monitored and expressed as standard parameters: spKt/V, spKt/V equilibrated (spKt/V-e), and the urea removal ratio (URR). Quality of life (QoL) was assessed by the questionnaire SF-36. A significant increase of F(max) (P = 0.040 in group ET; P = 0.032 in group EMS), of 6-min CWT (P < 0.001 in ET group; P = 0.042 in EMS group), and of W(peak) (P = 0.041 in ET group) was observed. In both exercising groups, significant increase of spKt/V, spKt/V-e, and URR was found as compared with initial values (P < 0.05). In both exercising groups, highly significant changes in summarized mental functions were found (P = 0.001); in summarized physical components, significant improvement was observed in the ET group (P = 0.006). Intradialytic RHB showed comparable positive effects on functional parameters, urea clearance, and QoL. Intradialytic EMS might represent wide therapeutic possibility in the near future.

• MeSH
• bérec MeSH
• chronické selhání ledvin patofyziologie   terapie   MeSH
• činnosti denního života MeSH
• dialýza ledvin škodlivé účinky   MeSH
• elektrická stimulace metody   MeSH
• ergometrie MeSH
• kosterní svaly fyziologie   MeSH
• kvalita života MeSH
• lidé středního věku MeSH
• lidé MeSH
• prospektivní studie MeSH
• průzkumy a dotazníky MeSH
• terapie cvičením metody   MeSH
• tolerance zátěže fyziologie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• randomizované kontrolované studie MeSH

To study the microvessels in bulbar conjunctiva, we conducted an experiment in goat with a pneumatically driven left heart bypass pump, which was replaced with an undulation pump-left ventricle assist device for 9 days. Three flow patterns were tested: complete pulsatile, continuous, and percentage of pulsatile. We studied the morphology of arterioles and venules of the bulbar conjunctiva using photograph records. The setting up of continuous flow caused global vasoconstriction (significant in venules-P < 0.05). During the pumping in the pulsatile and percentage of pulsatile modes, no significant changes of microvessel morphology were observed. The findings described could point to some disturbances in the microcirculatory bed in conditions of continuous flow.

• MeSH
• design vybavení MeSH
• hemodynamika MeSH
• konjunktiva krevní zásobení   MeSH
• kozy MeSH
• mikrocirkulace MeSH
• podpůrné srdeční systémy MeSH
• pulzatilní průtok MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH

• MeSH
• hemodynamika fyziologie   MeSH
• infekce spojené s protézou prevence a kontrola   MeSH
• modely nemocí na zvířatech MeSH
• skot MeSH
• umělé srdce MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH

• MeSH
• fyziologická adaptace fyziologie   MeSH
• hemodynamika fyziologie   MeSH
• modely nemocí na zvířatech MeSH
• nemoci srdce chirurgie   patofyziologie   MeSH
• pooperační komplikace MeSH
• skot MeSH
• umělé srdce škodlivé účinky   MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH

• MeSH
• biomedicínské inženýrství MeSH
• transplantace srdce MeSH
• umělé srdce trendy   využití   MeSH
• Publikační typ
• kongresy MeSH

• MeSH
• antihypertenziva farmakologie   MeSH
• elektronová mikroskopie MeSH
• játra ultrasonografie   MeSH
• ledviny ultrasonografie   MeSH
• míra přežití MeSH
• plíce ultrasonografie   MeSH
• srdce škodlivé účinky   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Geografické názvy
• Česká republika MeSH

• MeSH
• erytrocyty patologie   MeSH
• hemoglobiny analýza   MeSH
• hemolýza etiologie   MeSH
• skot MeSH
• umělé srdce škodlivé účinky   MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH

• MeSH
• transplantace srdce MeSH
• umělé srdce využití   MeSH