Diffuse large B-cell lymphoma (DLBCL) consists of at least two biologically and pathogenetically different subtypes, the germinal centre B-cell (GCB) and the activated B cell type (ABC). It has been suggested that immunohistochemistry can discriminate these subtypes as well. The aim of this study was to verify the validity of the most commonly used Hans algorithm in patients with DLBCL treated with anthracycline- based chemotherapy with rituximab. Immunohistochemical staining using standard protocols was performed on formalin fixed paraffin-embedded tissues. CD20, CD5, CD23, BCL2, CD10, BCL6, MUM1 and Ki67 antibodies were applied. Out of 120 examined cases 52 patients were evaluated as GCB type and 68 patients as having non-GCB, out of a set of 99 patients treated with immunochemotherapy 45 patients with GCB and 54 patients with non-GCB DLBCL were identified. In this set of patients, there was no statistically significant difference neither in overall survival (OS) (HR 1.47 95% CI 0.51-2.63; p=0.45) nor in progression free survival (PFS) (HR 1.57, 95 % CI 0.76-3.22; p=0.731) between both groups.

• MeSH
• Algorithms * MeSH
• Cyclophosphamide administration & dosage   MeSH
• Lymphoma, Large B-Cell, Diffuse drug therapy   mortality   pathology   MeSH
• Adult MeSH
• Doxorubicin administration & dosage   MeSH
• Immunoenzyme Techniques MeSH
• Middle Aged MeSH
• Humans MeSH
• Survival Rate MeSH
• Young Adult MeSH
• Antibodies, Monoclonal, Murine-Derived administration & dosage   MeSH
• Follow-Up Studies MeSH
• Prednisone administration & dosage   MeSH
• Prognosis MeSH
• Antineoplastic Combined Chemotherapy Protocols therapeutic use   MeSH
• Aged, 80 and over MeSH
• Aged MeSH
• Neoplasm Staging MeSH
• Vincristine administration & dosage   MeSH
• Germinal Center pathology   MeSH
• Check Tag
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Young Adult MeSH
• Male MeSH
• Aged, 80 and over MeSH
• Aged MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

OBJECTIVES: Maternal smoking has a negative effect on all stages of pregnancy. Tobacco smoke-related defects are well established at the clinical level; however, less is known about molecular mechanisms underlying these pathologic conditions. We thus performed a comprehensive analysis of transcriptome alterations induced by smoking in maternal and fetal cells. STUDY DESIGN: Samples of peripheral blood (PB), placenta (PL), and cord blood (UCB) were obtained from pregnant smokers (n = 20) and gravidas without significant exposure to tobacco smoke (n = 52). Gene expression profiles were assayed by Illumina Expression Beadchip v3 for analysis of 24,526 transcripts. The quantile method was used for normalization. Differentially expressed genes were analyzed in the Limma package and the P-values were corrected for multiple testing. Unsupervised hierarchical clustering was performed using average linkage and Euclidean distance. The enrichment of deregulated genes in biological processes was analyzed in DAVID database. RESULTS: Comparative analyses defined significant deregulation of 193 genes in PB, 329 genes in PL, and 49 genes in UCB of smokers. The deregulated genes were mainly related to xenobiotic metabolism, oxidative stress, inflammation, immunity, hematopoiesis, and vascularization. Notably, functional annotation of the affected genes identified several deregulated pathways associated with autoimmune diseases in the newborns of smokers. CONCLUSIONS: The study demonstrated maternal smoking causes significant changes in transcriptome of placental and fetal cells that deregulate numerous biological processes important for growth and development of the fetus. An activation of fetal CYP genes showed a limited ability of the placenta to modulate toxic effects of maternal tobacco use.

• MeSH
• Adult MeSH
• Fetal Blood metabolism   MeSH
• Cohort Studies MeSH
• Cotinine blood   MeSH
• Smoking adverse effects   blood   genetics   metabolism   MeSH
• Real-Time Polymerase Chain Reaction MeSH
• Humans MeSH
• Adolescent MeSH
• Young Adult MeSH
• Infant, Newborn MeSH
• Placenta metabolism   pathology   MeSH
• Fetus pathology   MeSH
• RNA chemistry   genetics   MeSH
• Oligonucleotide Array Sequence Analysis MeSH
• Pregnancy MeSH
• Transcriptome physiology   MeSH
• Check Tag
• Adult MeSH
• Humans MeSH
• Adolescent MeSH
• Young Adult MeSH
• Infant, Newborn MeSH
• Pregnancy MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

Primary mediastinal B-cell lymphoma (PMBL) seems to be reliably distinguished from diffuse large B-cell lymphoma (DLBCL) with microarray technology. We measured expression of Fcer2, Pdl2 and Blk genes using real-time quantitative polymerase chain reaction (RTqPCR) on formalin fixed, paraffin embedded material (FFPE) and suggested a formula to discriminate PMBL from DLBCL. For 39/82 included patients the diagnosis of PMBL was expected clinico-pathologically. Diagnosis of 10/39 and 2/43 of clinically considered PMBLs and DLBCLs, respectively, was not genetically confirmed. Compared to confirmed PMBLs, unconfirmed ones showed clinical features similar to DLBCLs, e.g. spleen infiltration (p=0,028) and decreased invasiveness in pericardium (p=0,045). They tended to have more common infradiaphragmatic involvement, less often tumor sclerosis or fluidothorax. There were no immunohistochemical differences between genetically confirmed and unconfirmed PMBLs. New approach of distinguishing PMBL and DLBCL is presented. It is based on expression of three genes in routinely available FFPE material using RTqPCR.

• MeSH
• Lymphoma, B-Cell diagnosis   MeSH
• Diagnosis, Differential MeSH
• Lymphoma, Large B-Cell, Diffuse diagnosis   MeSH
• Adult MeSH
• Immunohistochemistry MeSH
• Middle Aged MeSH
• Humans MeSH
• Mediastinal Neoplasms diagnosis   MeSH
• Polymerase Chain Reaction methods   MeSH
• Check Tag
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Male MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

• MeSH
• Hexoses chemistry   MeSH
• Lactams chemistry   MeSH
• Magnetic Resonance Spectroscopy methods   MeSH
• Carbohydrates analysis   chemistry   MeSH

• MeSH
• DNA-Binding Proteins chemistry   metabolism   MeSH
• Fungal Proteins chemistry   MeSH
• Leucine Zippers MeSH
• Oligodeoxyribonucleotides chemistry   MeSH
• Oligopeptides chemistry   metabolism   MeSH
• Protein Kinases chemistry   metabolism   MeSH
• Transcription Factors genetics   MeSH

• MeSH
• Distamycins chemistry   metabolism   MeSH
• DNA chemistry   metabolism   MeSH
• Protein Conformation MeSH
• Netropsin chemistry   metabolism   MeSH
• Peptides chemical synthesis   chemistry   metabolism   MeSH
• Protein Binding MeSH
• Base Composition MeSH

• MeSH
• Circular Dichroism MeSH
• Nucleic Acid Conformation MeSH
• Molecular Sequence Data MeSH
• Oligonucleotides chemical synthesis   chemistry   MeSH
• Base Sequence MeSH
• Base Composition MeSH

• MeSH
• Circular Dichroism MeSH
• Nucleic Acid Conformation MeSH
• Molecular Sequence Data MeSH
• Oligopeptides chemistry   MeSH
• Polydeoxyribonucleotides chemistry   MeSH
• Temperature MeSH

• MeSH
• Methanol MeSH
• Oligonucleotides chemistry   MeSH
• Polydeoxyribonucleotides chemistry   MeSH
• Water MeSH

• MeSH
• DNA MeSH
• Nucleic Acids MeSH
• Spectrophotometry MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Comparative Study MeSH