Species of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex are important human pathogens which can be recovered from animals and food, potential sources for their dissemination. The aim of the present study was to characterise the Acinetobacter isolates recovered from market meat samples in Peru. From July through August 2012, 138 meat samples from six traditional markets in Lima were cultured in Lysogeny and Selenite broths followed by screening of Gram-negative bacteria in selective media. Bacterial isolates were identified by MALDI-TOF MS and DNA-based methods and assessed for their clonal relatedness and antimicrobial susceptibility. Twelve Acinetobacter isolates were recovered from calf samples. All but one strain were identified as members of the clinically-relevant Acinetobacter calcoaceticus-Acinetobacter baumannii complex: 9 strains as Acinetobacter pittii, 1 strain as A. baumannii, and 1 strain as the recently described novel species A. dijkshoorniae. The remaining strain could not be identified at the species level unambiguously but all studies suggested close relatedness to A. bereziniae. All isolates were well susceptible to antibiotics. Based on macrorestriction analysis, six isolates were further selected and some of them were associated with novel MLST profiles. The presence of pathogenic Acinetobacter species in human consumption meat might pose a risk to public health as potential reservoirs for their further spread into the human population. Nevertheless, the Acinetobacter isolates from meat found in this study were not multidrug resistant and their prevalence was low. To our knowledge, this is also the first time that the A. dijkshoorniae species is reported in Peru.
- MeSH
- Acinetobacter drug effects genetics isolation & purification pathogenicity MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Acinetobacter Infections microbiology veterinary MeSH
- Food Contamination analysis MeSH
- Humans MeSH
- Meat microbiology MeSH
- Microbial Sensitivity Tests MeSH
- Multilocus Sequence Typing MeSH
- Cattle Diseases microbiology MeSH
- Cattle MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Peru MeSH
- MeSH
- Acinetobacter pathogenicity drug effects MeSH
- Drug Resistance, Bacterial * MeSH
- Enterococcus pathogenicity drug effects MeSH
- Escherichia coli pathogenicity drug effects MeSH
- Klebsiella pneumoniae pathogenicity drug effects MeSH
- Humans MeSH
- Pseudomonas aeruginosa pathogenicity drug effects MeSH
- Staphylococcus aureus pathogenicity drug effects MeSH
- Streptococcus pneumoniae pathogenicity drug effects MeSH
- Population Surveillance * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
- Geographicals
- Czech Republic MeSH
Študovali sme rast, povrchové vlastnosti, produkciu lipázy, histamínu a niektorých signálnychmolekúl (N-acylované homoserín laktóny, AHL) u štyroch kmeňov Acinetobacter baumannii, jednéhokmeňa Acinetobacter lwoffii a jedného kmeňa Acinetobacter haemolyticus kultivovaných v trochkomplexných médiách. Intenzívnejší rast bakteriálnych suspenzií sme zistili vMuellerovom-Hintonovombujóne a v mozgovo-srdcovej infúzii v porovnaní s rastom v proteózovom peptóne. Vyššínárast bakteriálnych kmeňov v týchto dvoch médiách bol spojený aj s výraznejšou hydrofóbnosťoupovrchu baktérií (s výnimkou A. haemolyticus) vyjadrenou ich adherenciou na xylén. Najvyššiuhladinu lipázy sme zistili u všetkých kmeňov (s výnimkou A. haemolyticus) po kultivácii v Muellerovom-Hintonovom bujóne. Zloženie kultivačného média neovplyvnilo produkciu histamínu. Anijeden študovaný kmeň v žiadnom testovanom médiu neprodukoval krátko reťazcové nesubstituovanéAHL, ktoré môžu byť jednými zo signálnych molekúl podieľajúcich sa na komunikácii buniek(cell- to-cell communication). Rast, povrchová hydrofobicita a aktivita lipázy u kmeňov Acinetobacterspp. boli ovplyvnené zložením kultivačného média.
Growth, surface properties, production of lipase, histamine and some signal molecules (N-acyl-homoserinelactones, AHLs) in four Acinetobacter baumannii strains, in one Acinetobacter lwoffii andin one Acinetobacter haemolyticus strain cultivated in three complex media were studied. Moreintensive growth of bacterial suspensions was observed inMueller-Hinton broth and in brain heartinfusion in comparison with growth in proteose peptone. Greater growth of bacterial strains (withthe exception of A. haemolyticus) in these two media was associated with more significant surfacehydrophobicity of bacteria manifested by their adherence to xylene. A higher level of lipase wasfound in all strains (with the exception of A. haemolyticus) after cultivation inMueller-Hinton broth.Composition of culture medium did not affect production of histamine. None of the studied strainsin any medium tested produced short-chained unsubstituted AHLs that can be ones of the signalmolecules shared with cell-to-cell communication.
- MeSH
- Acinetobacter physiology pathogenicity growth & development MeSH
- Bacteriological Techniques methods MeSH
- Histamine MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Culture Media chemistry MeSH
- Lactones MeSH
- Humans MeSH
- Lipase MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
- Comparative Study MeSH
Autorka podáva prehľad novších poznatkov o príslušníkoch rodu Acinetobacter. Hodnotí sa najmäich účasť na nozokomiálnych infekciách, rezistencia na antibiotiká, faktory virulencie ako ajmožnosti ovplyvnenia ich virulencie antibiotikami.
The author reviewed recent data on the genus Acinetobacter. It is an evaluation of their participationin nosocomial infections, antibiotic resistance, virulence factors as well as the possibilities ofmodifications of their virulence after treatment with antibiotics.
Testovali sme citlivosť na baktericídny účinok séra a povrchovú hydrofobicitu kmeňov Acinetobac-ter baumannii izolovaných z močovej a z respiračnej sústavy pacientov po účinku imipenemuv subinhibičných koncentráciách (sub-MIC) (1/4, 1/8 a 1/16 MIC). Antibiotikum v uvedených koncen-tráciách významne znížilo citlivosť kmeňa z močovej sústavy na baktericídnu aktivitu ľudskéhoséra. Citlivosť na baktericídny účinok séra kmeňa z respiračnej sústavy bola ovplyvnená len veľminevýrazne. Imipenem v koncentrácii 1/4 MIC znížil povrchovú hydrofobicitu na 76,1 % (kmeňz močovej sústavy) a na 81,9 % (kmeň z respiračnej sústavy) kontrolných hodnôt (bez imipenemu).Nižšie koncentrácie antibiotika ovplyvnili bunkovú povrchovú hydrofobicitu len v menšom rozsa-hu. Možné zníženie citlivosti baktérií na baktericídny účinok séra po účinku sub-MIC imipenemuaj v pokusoch in vivo by mohlo znamenať zvýšenie virulencie týchto kmeňov.
Sensitivity to the serum bactericidal activity and surface hydrophobicity of Acinetobacter bauman-nii strains isolated from the urinary (UT) or respiratory tract (RT) of patients after exposure toimipenem at subinhibitory concentrations (sub-MICs) (1/4, 1/8 or 1/16 of their MICs) were tested. Theantibiotic at the mentioned concentrations decreased significantly the sensitivity of strain UT tothe bactericidal activity of human serum. Sensitivity to the bactericidal activity of serum of strainRT was affected only very ineffectively. Imipenem at 1/4 of their MICs reduced the cell surfacehydrophobicity to 76.1% (strain UT) and to 81.9% (strain RT) compared with control values (withoutimipenem). Lower concentrations of antibiotic influenced cell surface hydrophobicity only toa smaller extent. Possible decrease in sensitivity of bacteria to the bactericidal activity of serumafter treatment with imipenem in „in vivo“ experiments would manifest an increase in virulence ofthese strains.
- MeSH
- Acinetobacter pathogenicity MeSH
- Humans MeSH
- Serum Bactericidal Test MeSH
- In Vitro Techniques MeSH
- Check Tag
- Humans MeSH
APMIS ; Supplementum Vol. 102. 47
[1st ed.] 41 s. : tab., grafy ; 26 cm
