Background: Sanguinarine (SG) has been reported to form DNA adducts in vitro and increase the levels of DNAsingle strand breaks in the blood and bone marrow of mice treated intraperitoneally with SG. Recently, we showedno genotoxic eff ects of orally administrated 120 mg/kg feed Macleaya cordata extract (a mixture of sanguinarineand chelerythrine) in pigs or rats in 90-day studies. The goal of this paper was to assess the possible genotoxicity ofM. cordata extract when included as a dietary admixture to rodents at concentrations providing 600 mg/kg feed and100, 7000 or 14000 mg/kg feed Sangrovit® (natural feed additive containing M. cordata extract and powdered M.cordata) in a 90-day pilot study.Methods and Results: The rats consumed ad libitum either the standard diet or the diets containing 367 ppm of sanguinarineand chelerythrine in M. cordata extract, and 5, 330, or 660 ppm of total alkaloids in Sangrovit® for 90 days.The DNA adducts formation in liver was analyzed by 32P-postlabeling technique and DNA single strand breaks inlymphocytes were evaluated by Comet assay. The results showed that M. cordata extract and/or Sangrovit® inducedno DNA damage to rat lymphocytes or hepatocytes after 90-days oral administration.Conclusions: Data from the studies described in this paper and the fact that Sangrovit® given to the rats in ourexperiments were higher than the recommended dose (50 to 100 mg/kg feed), argue strongly in favour of the use of Sangrovit® in live stock.
- MeSH
- barvení a značení metody využití MeSH
- benzofenantridiny aplikace a dávkování diagnostické užití toxicita MeSH
- financování organizované MeSH
- hepatocyty účinky léků MeSH
- isochinoliny aplikace a dávkování diagnostické užití toxicita MeSH
- játra účinky léků MeSH
- kometový test metody využití MeSH
- léky rostlinné čínské farmakologie toxicita MeSH
- lymfocyty účinky léků MeSH
- Papaveraceae genetika toxicita MeSH
- potkani Wistar MeSH
- potravinářské přísady MeSH
- rostlinné extrakty genetika toxicita MeSH
- testy genotoxicity metody využití MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
This review provides a brief information on principles and possible applications of the comet assay method, known as single cell gel electrophoresis, allowing to analyze DNA damage. This method developed in the mid-seventies was widely used in the early eighties of the last century. Several modifications of the comet assay approach made this method an attractive tool in various fields such as molecular biology research, quality control, clinical diagnosis, and monitoring of environmental pollution.
Oxidative stress has been implicated to play a major role in aging and age-related diseases. In the present study, we investigated the effects of aging on the total antioxidant capacity, uric acid, lipid peroxidation, total sulfhydryl group content and damage to DNA in adult (6 months), old (15 months) and senescent (26 months) male Wistar rats. The antioxidant capacity, determined by phycoerythrin-based TRAP method (total peroxyl radical-trapping potential) was significantly decreased in the plasma and myocardium of old and senescent rats, whereas plasma level of uric acid was elevated in 26-month-old rats. Age-related decline in plasma and heart antioxidant capacity was accompanied by a significant loss in total sulfhydryl group content, increased lipid peroxidation and higher DNA damage in lymphocytes. Correlations between TRAP and oxidative damage to lipids, proteins and DNA suggest that the decline in antioxidant status may play an important role in age-related accumulation of cell damage caused by reactive oxygen species.
- MeSH
- antioxidancia metabolismus terapeutické užití MeSH
- financování organizované využití MeSH
- kometový test metody využití MeSH
- kyselina močová metabolismus MeSH
- oxidační stres genetika účinky záření MeSH
- peroxidace lipidů genetika účinky záření MeSH
- potkani Wistar MeSH
- reaktivní formy kyslíku metabolismus škodlivé účinky MeSH
- stárnutí fyziologie metabolismus účinky léků MeSH
- sulfhydrylové sloučeniny metabolismus škodlivé účinky MeSH
- MeSH
- adjuvancia imunologická MeSH
- antikarcinogenní látky MeSH
- antimutagenní látky MeSH
- antioxidancia * MeSH
- beta-glukany * MeSH
- imunologické faktory MeSH
- kometový test metody přístrojové vybavení využití MeSH
- konformace nukleové kyseliny * MeSH
- lidé MeSH
- magnetická rezonanční spektroskopie metody přístrojové vybavení MeSH
- plazmidy MeSH
- poškození DNA MeSH
- radioprotektivní látky MeSH
- Check Tag
- lidé MeSH
- MeSH
- antioxidancia terapeutické užití MeSH
- dieta vegetariánská MeSH
- genetické markery genetika imunologie účinky léků MeSH
- kometový test metody trendy využití MeSH
- lidé MeSH
- molekulární epidemiologie MeSH
- oxidační stres imunologie účinky léků účinky záření MeSH
- poškození DNA genetika imunologie MeSH
- reaktivní formy kyslíku imunologie metabolismus škodlivé účinky MeSH
- Check Tag
- lidé MeSH
Basic & clinical pharmacology & toxicology, ISSN 1742-7835 vol. 96, suppl. I, 2005
42 s. : il.,tab. ; 28 cm
