Background: Sanguinarine (SG) has been reported to form DNA adducts in vitro and increase the levels of DNAsingle strand breaks in the blood and bone marrow of mice treated intraperitoneally with SG. Recently, we showedno genotoxic eff ects of orally administrated 120 mg/kg feed Macleaya cordata extract (a mixture of sanguinarineand chelerythrine) in pigs or rats in 90-day studies. The goal of this paper was to assess the possible genotoxicity ofM. cordata extract when included as a dietary admixture to rodents at concentrations providing 600 mg/kg feed and100, 7000 or 14000 mg/kg feed Sangrovit® (natural feed additive containing M. cordata extract and powdered M.cordata) in a 90-day pilot study.Methods and Results: The rats consumed ad libitum either the standard diet or the diets containing 367 ppm of sanguinarineand chelerythrine in M. cordata extract, and 5, 330, or 660 ppm of total alkaloids in Sangrovit® for 90 days.The DNA adducts formation in liver was analyzed by 32P-postlabeling technique and DNA single strand breaks inlymphocytes were evaluated by Comet assay. The results showed that M. cordata extract and/or Sangrovit® inducedno DNA damage to rat lymphocytes or hepatocytes after 90-days oral administration.Conclusions: Data from the studies described in this paper and the fact that Sangrovit® given to the rats in ourexperiments were higher than the recommended dose (50 to 100 mg/kg feed), argue strongly in favour of the use of Sangrovit® in live stock.
- MeSH
- Staining and Labeling methods utilization MeSH
- Benzophenanthridines administration & dosage diagnostic use toxicity MeSH
- Financing, Organized MeSH
- Hepatocytes drug effects MeSH
- Isoquinolines administration & dosage diagnostic use toxicity MeSH
- Liver drug effects MeSH
- Comet Assay methods utilization MeSH
- Drugs, Chinese Herbal pharmacology toxicity MeSH
- Lymphocytes drug effects MeSH
- Papaveraceae genetics toxicity MeSH
- Rats, Wistar MeSH
- Food Additives MeSH
- Plant Extracts genetics toxicity MeSH
- Mutagenicity Tests methods utilization MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
This review provides a brief information on principles and possible applications of the comet assay method, known as single cell gel electrophoresis, allowing to analyze DNA damage. This method developed in the mid-seventies was widely used in the early eighties of the last century. Several modifications of the comet assay approach made this method an attractive tool in various fields such as molecular biology research, quality control, clinical diagnosis, and monitoring of environmental pollution.
Oxidative stress has been implicated to play a major role in aging and age-related diseases. In the present study, we investigated the effects of aging on the total antioxidant capacity, uric acid, lipid peroxidation, total sulfhydryl group content and damage to DNA in adult (6 months), old (15 months) and senescent (26 months) male Wistar rats. The antioxidant capacity, determined by phycoerythrin-based TRAP method (total peroxyl radical-trapping potential) was significantly decreased in the plasma and myocardium of old and senescent rats, whereas plasma level of uric acid was elevated in 26-month-old rats. Age-related decline in plasma and heart antioxidant capacity was accompanied by a significant loss in total sulfhydryl group content, increased lipid peroxidation and higher DNA damage in lymphocytes. Correlations between TRAP and oxidative damage to lipids, proteins and DNA suggest that the decline in antioxidant status may play an important role in age-related accumulation of cell damage caused by reactive oxygen species.
- MeSH
- Antioxidants metabolism therapeutic use MeSH
- Financing, Organized utilization MeSH
- Comet Assay methods utilization MeSH
- Uric Acid metabolism MeSH
- Oxidative Stress genetics radiation effects MeSH
- Lipid Peroxidation genetics radiation effects MeSH
- Rats, Wistar MeSH
- Reactive Oxygen Species metabolism adverse effects MeSH
- Aging physiology metabolism drug effects MeSH
- Sulfhydryl Compounds metabolism adverse effects MeSH
- MeSH
- Adjuvants, Immunologic MeSH
- Anticarcinogenic Agents MeSH
- Antimutagenic Agents MeSH
- Antioxidants * MeSH
- beta-Glucans * MeSH
- Immunologic Factors MeSH
- Comet Assay methods instrumentation utilization MeSH
- Nucleic Acid Conformation * MeSH
- Humans MeSH
- Magnetic Resonance Spectroscopy methods instrumentation MeSH
- Plasmids MeSH
- DNA Damage MeSH
- Radiation-Protective Agents MeSH
- Check Tag
- Humans MeSH
- MeSH
- Antioxidants therapeutic use MeSH
- Diet, Vegetarian MeSH
- Genetic Markers genetics immunology drug effects MeSH
- Comet Assay methods trends utilization MeSH
- Humans MeSH
- Molecular Epidemiology MeSH
- Oxidative Stress immunology drug effects radiation effects MeSH
- DNA Damage genetics immunology MeSH
- Reactive Oxygen Species immunology metabolism adverse effects MeSH
- Check Tag
- Humans MeSH
Basic & clinical pharmacology & toxicology, ISSN 1742-7835 vol. 96, suppl. I, 2005
42 s. : il.,tab. ; 28 cm
