- Klíčová slova
- sarafotoxin,
- MeSH
- centrální nervový systém účinky léků MeSH
- endokrinní systém účinky léků MeSH
- endoteliny biosyntéza fyziologie MeSH
- kardiovaskulární systém účinky léků MeSH
- ledviny účinky léků MeSH
- lidé MeSH
- Viperidae MeSH
- zmijí jedy farmakologie chemie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- přehledy MeSH
The product of the retinoblastoma susceptibility gene, pRb, is a negative regulator of cell growth. It functions by regulating the activity of transcription factors. Rb represses some genes by sequestering or inactivating the positive transcription factor E2F and seems to activate some others by interacting with factors like Sp1 or ATF-2. However, there are only a few examples of genes which are positively regulated by pRb. In order to find out if there are common mechanisms for promoter regulation by pRb, we were interested to identify more genes which are either stimulated or repressed by pRb. Using the method of differential display (DDRT-PCR) in combination with nuclear run-on analyses we were able to detect a number of genes which are upregulated by ectopic expression of the Rb gene in Rb-deficient mammary carcinoma cells. We could demonstrate not only stimulation of the endogenous mutant Rb gene but also positive regulation of genes coding for diverse classes of proteins, including the endothelial growth regulator endothelin-1 and the proteoglycans versican and PG40. As a second approach, we investigated gene expression in cell lines established from Rb deficient heterozygous and homozygous knockout mouse embryos and normal mice. We have identified several genes the expression of which correlates positively or negatively with the presence of Rb. These data provide further evidence for pRb being a master regulator of a complex network of gene activities defining the difference between dividing and resting or differentiated cells.
- MeSH
- endoteliny biosyntéza genetika MeSH
- geny retinoblastomu fyziologie MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- myši knockoutované MeSH
- myši MeSH
- nádorové buňky kultivované MeSH
- polymerázová řetězová reakce metody MeSH
- proteoglykany biosyntéza genetika MeSH
- regulace genové exprese * MeSH
- retinoblastomový protein genetika fyziologie MeSH
- sekvence nukleotidů MeSH
- transfekce MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
