16th ed. 2 sv.
26 sv.
- MeSH
- Chemistry Techniques, Analytical MeSH
- Publication type
- Periodical MeSH
- Conspectus
- Chemie. Mineralogické vědy
- NML Fields
- chemie, klinická chemie
sv.
- MeSH
- Chemistry Techniques, Analytical MeSH
- Publication type
- Periodical MeSH
- Conspectus
- Chemie. Mineralogické vědy
- NML Fields
- chemie, klinická chemie
3rd ed. vii, 187 s.
16th ed. 2 sv. (různé stránkování)
- MeSH
- Chemistry, Clinical MeSH
- Conspectus
- Veřejné zdraví a hygiena
- NML Fields
- ekologie, životní prostředí a jeho ochrana
- chemie klinická
The concentration of carotenoids and fat-soluble vitamins in human plasma may play a significant role in numerous chronic diseases such as age-related macular degeneration and some types of cancer. Although these compounds are of utmost interest for human health, methods for their simultaneous determination are scarce. A new high pressure liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) method for the quantification of selected carotenoids and fat-soluble vitamins in human plasma was developed, validated, and then applied in a pilot dietary intervention study with healthy volunteers. In 50 min, 16 analytes were separated with an excellent resolution and suitable MS signal intensity. The proposed HPLC-MS/MS method led to improvements in the limits of detection (LOD) and quantification (LOQ) for all analyzed compounds compared to the most often used HPLC-DAD methods, in some cases being more than 100-fold lower. LOD values were between 0.001 and 0.422 µg/mL and LOQ values ranged from 0.003 to 1.406 µg/mL, according to the analyte. The accuracy, precision, and stability met with the acceptance criteria of the AOAC (Association of Official Analytical Chemists) International. According to these results, the described HPLC-MS/MS method is adequately sensitive, repeatable and suitable for the large-scale analysis of compounds in biological fluids.
- MeSH
- Carotenoids blood MeSH
- Humans MeSH
- Limit of Detection MeSH
- Pilot Projects MeSH
- Tandem Mass Spectrometry methods MeSH
- Vitamins blood MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Validation Study MeSH
OBJECTIVE: The aim of this study was to determine whether Microdispersed Oxidized Cellulose (MDOC) possesses a hypolipidemic effect in apolipoprotein-E/low-density lipoprotein receptor double-knockout (ApoE/LDLR-deficient) mice and the possible mechanism of this effect in mice. METHODS: Female ApoE/LDLR-deficient mice subdivided into two groups were fed with a Western-type diet for 8 wk, and the experimental group was supplemented with 5% MDOC for 8 wk. Female C57BL/6J mice were fed an atherogenic diet containing 5% MDOC or pectin for the determination of a possible hypolipidemic mechanism of MDOC action. RESULTS: Biochemical analysis showed that 5% MDOC treatment significantly decreased total cholesterol by 20% (P = 0.0338) and very-LDL cholesterol by 21% (P = 0.0110) and significantly increased the level of high-density lipoprotein cholesterol by 62% (P = 0.0172) when compared with non-treated ApoE/LDLR-deficient mice. The results Association of Official Analytical Chemists method 991.43 revealed that MDOC contains 59.78 +/- 5.0% of fiber. Furthermore, it was demonstrated that administration of MDOC did not affect cholesterol absorption in the small intestine. Using C57BL/6J mice, MDOC and pectin treatments decreased cholesterol content in liver and increased fermentation in the gut in vivo. In vitro experiments confirmed that MDOC is fermentable under conditions mimicking those in the large intestine. CONCLUSION: We demonstrated hypolipidemic effects of MDOC in ApoE/LDLR-deficient mice. Moreover, we propose that MDOC is a hypolipidemic soluble fiber acting probably by increased fermentation and production of short-chain fatty acids in the large intestine in mice. We propose that MDOC might be a possible source of soluble fiber for use in dietary supplements.
- MeSH
- Apolipoproteins E genetics deficiency MeSH
- Cellulose, Oxidized pharmacology MeSH
- Cholesterol blood MeSH
- Fermentation MeSH
- Cholesterol, HDL blood MeSH
- Hypolipidemic Agents pharmacology MeSH
- Intestinal Absorption physiology drug effects MeSH
- Fatty Acids, Volatile analysis MeSH
- Receptors, LDL genetics deficiency MeSH
- Disease Models, Animal MeSH
- Mice, Inbred C57BL MeSH
- Mice, Knockout MeSH
- Mice MeSH
- Random Allocation MeSH
- Dietary Fiber administration & dosage pharmacology MeSH
- Intestine, Large metabolism MeSH
- Cholesterol, VLDL blood MeSH
- Treatment Outcome MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
