"NR8048" Dotaz Zobrazit nápovědu
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
181 l. : il., tab. ; 30 cm
The monolithic columns represent the new revolution trend in chromatography. The discovery of this technology opens the new possibilities in analytical chemistry. The use of the modern monolithic technology in HPLC analysis of biological active substances for clinical research of cardiovascular, cancer and metabolic diseases in gerontology. To develop the modern analytical procedures in clinical studies and to improve the quality of diagnosis of chronically illnesses and care with aim to reduce the expenses of the treatment in the elderly.
Monolitní kolony představují revoluční směr v chromatografii. Objev této technologie otevírá nové možnosti aplikací analytické chemie v klinické medicíně. Cílem projektu je využití moderních monolitních technologií pro HPLC analýzu biologicky aktivních látek v klinickém sledování, diagnostice a monitoraci průběhu onkologických, kardiovaskulárních a metabolických chorob u pacientů z vyšších věkových skupin. Zlepšení laboratorní diagnostiky chronických chorob aplikací moderních analytických postupů v klinickém sledování dosáhnout zefektivnění výdajů na péči o starší populaci a zkvalitnění péče o tyto pacienty.
- MeSH
- biologické faktory analýza MeSH
- chemické techniky analytické metody MeSH
- kardiovaskulární nemoci diagnóza MeSH
- klinická chemie metody trendy MeSH
- klinické laboratorní techniky metody trendy MeSH
- metabolické nemoci diagnóza MeSH
- nádory diagnóza MeSH
- neopterin MeSH
- senioři MeSH
- stárnutí fyziologie MeSH
- vitamin E MeSH
- vysokoúčinná kapalinová chromatografie metody trendy využití MeSH
- Check Tag
- senioři MeSH
- Konspekt
- Fyziologie člověka a srovnávací fyziologie
- NLK Obory
- biochemie
- chemie, klinická chemie
- geriatrie
- chemie, klinická chemie
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
- MeSH
- financování organizované MeSH
- Publikační typ
- abstrakty MeSH
In this study a novel, simple and rapid reversed-phase high performance liquid chromatography (HPLC) procedure for simultaneous determination of vitamins A and E (retinol and alpha-tocopherol) in blood serum has been developed and validated using monolithic column and diode-array detection (DAD). The monolithic column Chromolith Performance RP-18e (100 mm x 4.6 mm) was operated at ambient temperature. One hundred percent methanol at flow rate 2.5 ml min(-1) was used as a mobile phase. Detection of both compounds was performed with diode-array detector, retinol was monitored at 325 nm and alpha-tocopherol at 295 nm. The linear dependence between peak area and concentration ranged from 0.25 to 10.00 micromol l(-1) for retinol and 0.5-50.0 micromol l(-1) for alpha-tocopherol. The limit of detection (LOD) for retinol was 0.02 micromol l(-1) and limit of quantification (LOQ) was 0.07 micromol l(-1). The limit of detection (LOD) for alpha-tocopherol was 0.1 micromol l(-1) and limit of quantification (LOQ) was 0.3 micromol l(-1). Retinol was eluted in 0.8 min and alpha-tocopherol in 1.4 min. The simultaneous analysis of vitamin A and E can be achieved in less than 2 min. The implementation of monolithic column Chromolith Performance shortens the time of analysis of both vitamins four times in comparison with using traditional particulate column Pecosphere C18 (150 mm x 4.6 mm), 5 microm. This fact may play an important role for routine clinical analysis of biological samples
Among other side effects, administration of anticancer agents is accompanied by manifestations of gastrointestinal toxicity and disturbances of antioxidant balance. The monitoring of these toxic effects in clinical practice is impeded by a dearth of reliable laboratory methods. Therefore, a simple and rapid reversed-phase high-performance liquid chromatography procedure for selective and sensitive determination of retinol, a-tocopherol, and retinyl esters (retinyl-palmitate and retinyl-stearate) in blood serum has been developed and presented in this study. A Series 200 LC HPLC instrument from Perkin Elmer (Norwalk, USA) with diode-array detector (DAD) was used for the analysis. Separations of retinol, alpha-tocopherol, retinyl-palmitate, and retinyl-stearate were performed using a Chromolith Performance RP-18e, 100 x 4.6 mm monolithic column from Merck (Darmstadt, Germany). Gradient elution was used at a flow rate of 3 mL/min; the mobile phase was methanol-water (95:5, v/v) for 0-2.1 min and methanol-2-propanol (60:40, v/v) for 2.1-4.9 min. The total time of analysis was 6 min. The injection volume was 20 microL and the analysis was performed at ambient temperature. Detection of retinol, alpha-tocopherol, and retinyl esters was carried out at 325, 295, and 330 nm, respectively. For practical assessment of the method, the vitamin A absorption test was performed on seven healthy controls as well as on six patients with non-small cell lung carcinoma or head and neck carcinoma previously treated by chemotherapy and/or radiotherapy, six patients with rectal carcinoma before chemoradiotherapy, four patients with gastrointestinal stromal tumor (GIST) before treatment with imatinib, and a breast cancer patient with chemotherapy-induced diarrhea. Present data demonstrate the feasibility of large scale HPLC determination of vitamin E, vitamin A, and retinyl esters in human serum using a silica monolithic column, and this method may represent a valuable aid in the laboratory monitoring of the toxicity of anticancer therapy.
- MeSH
- absorpce MeSH
- časové faktory MeSH
- chromatografie metody MeSH
- estery * analýza krev MeSH
- gastrointestinální stromální tumory farmakoterapie MeSH
- kalibrace MeSH
- lidé MeSH
- monitorování léčiv * metody MeSH
- piperaziny farmakologie škodlivé účinky MeSH
- protinádorové látky * farmakologie škodlivé účinky MeSH
- průjem chemicky indukované MeSH
- pyrimidiny farmakologie škodlivé účinky MeSH
- reprodukovatelnost výsledků MeSH
- vitamin A * MeSH
- vysokoúčinná kapalinová chromatografie metody přístrojové vybavení MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Inhalational administration of interleukin-2 (IL-2) is effective in controlling renal cell carcinoma (RCC) lung metastases with minimal toxicity. Neopterin is an indicator of systemic immune activation in metastatic cancer and is increased after systemic IL-2 administration. Urinary neopterin was investigated in 13 patients with metastatic RCC and 18 controls. In seven patients, urinary neopterin was followed before and after treatment with inhalational IL-2. Neopterin was measured by high-performance liquid chromatography and creatinine was determined by Jaffé reaction. Urinary neopterin was significantly increased in patients with metastatic RCC compared to controls (257 +/- 263 micromol/mol creatinine vs. 110 +/- 41 micromol/mol creatinine; Mann-Whitney U-test, p < 0.05). Median survival was significantly longer in patients with urinary neopterin <173 micromol/mol creatinine compared to patients with neopterin > or = 173 micromol/mol creatinine (698 vs. 245 days; log-rank test, p < 0.05). No significant increase was observed after inhalational IL-2 therapy (147 +/- 101 vs. 153 +/- 54 micromol/mol creatinine). We conclude that urinary neopterin is increased in patients with metastatic RCC, and higher neopterin concentrations are indicative of poor prognosis. The absence of an increase in urinary neopterin after inhalational IL-2 therapy is in accord with the lack of significant systemic toxicity. Copyright 2005 John Wiley & Sons, Ltd.
- MeSH
- aplikace inhalační MeSH
- dospělí MeSH
- financování organizované MeSH
- interleukin-2 aplikace a dávkování terapeutické užití MeSH
- karcinom z renálních buněk farmakoterapie moč MeSH
- lidé středního věku MeSH
- lidé MeSH
- neopterin moč MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- abstrakt z konference MeSH
