1. The project is designated to identify the optimal heart rate during controlled exercise aimed at beneficial modulation of lipid parameters and insulin sensitivity. 2. The influence of I/D variant in ACE in combiantion with exercise in obeses for finally changes insulin resistancy.

1. Bude určeno optimum tepové frekvence při kontrolované fyzické zátěži pro pozitivní ovlivnění lipidových parametrů a citlivosti k inzulínu. Zjistit vliv genetických dispozic na ovlivnění biochemických parametrů fyzickou aktivitou. 2. Bude zjištěno jakovlivňuje I/D varianta v ACE výslednou změnu v inzulinové rezistenci v kombinaci s fyzickou zátěží u obézních.

• MeSH
• inhibitory ACE MeSH
• inzulinová rezistence MeSH
• kardiovaskulární nemoci metabolismus   MeSH
• lipidy MeSH
• obezita patofyziologie   MeSH
• pohybová aktivita MeSH
• polymorfismus genetický MeSH
• porucha glukózové tolerance MeSH
• redukční dieta MeSH
• rizikové faktory MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• vnitřní lékařství
• biologie
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR

Obesity is a serious health problem worldwide and many genes have been implicated in determination of obesity, but our knowledge of the genes responsible for individual differences in weight loss after physical intervention are poor. One of the candidate genes is a gene for angiotensin-converting enzyme (ACE) ant its insertion/deletion (I/D) polymorphism. We have analyzed the association between the ACE gene variant in intervened obese females. Twenty four unrelated healthy obese (BMI > 29.9 kg/m2, with abdominal type of obesity) premenopausal (age between 25 and 45 years) Czech Caucasian sedentary and non-diabetic females, pre-selected according the ACE I/D polymorphism (twelve II and twelve DD homozygotes) were studied in a medical research centre. They underwent 9 weeks intervention program (combination of the lowering of dietary intake to optimal level for the age and 3 times a week physical activity at fitness centre). The participants were supervised to sustain a heart rate of 65 % of maximum. Anthropometrical, biochemical parameters and body composition (Bodystat 1500) were analyzed before and after the intervention. Our study suggest, that in Czech Caucasian females I/D polymorphism within the ACE gene will have no major effect on weight loss. Interestingly, we have detected, that in obese females II genotype was associated with higher increase in basal metabolic rate (202 kcal per day) then in DD homozygotes (p<0.05), thus at least under some circumstances, this genetic variant may have an slight effect on BMI development.

• MeSH
• angiotensin konvertující enzym genetika   MeSH
• břicho MeSH
• dospělí MeSH
• financování organizované MeSH
• frekvence genu MeSH
• genetická predispozice k nemoci MeSH
• hmotnostní úbytek MeSH
• homozygot MeSH
• index tělesné hmotnosti MeSH
• kombinovaná terapie MeSH
• lidé středního věku MeSH
• lidé MeSH
• obezita MeSH
• polymorfismus genetický MeSH
• redukční dieta MeSH
• rizikové faktory MeSH
• terapie cvičením MeSH
• výsledek terapie MeSH
• životní styl etnologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Geografické názvy
• Česká republika MeSH

High plasma levels of triglycerides (TG) are an independent risk factor in the development of cardiovascular disease, with about 50 % of the final levels being determined genetically. Apolipoprotein A5 (APOA5) is the last discovered member of the apolipoprotein APOA1/C3/A4 gene cluster, found by comparative sequencing analysis. The importance of APOA5 gene for determination of plasma triglyceride levels has been suggested after development of transgenic and knock-out mice (transgenic mice displayed significantly reduced TG, whereas knock-out mice had high TG). In Czech population, alleles C-1131 and Trp19 are associated with elevated levels of plasma TG and higher risk of myocardial infarction development. These alleles also play some role in nutrigenetics and actigenetics of lifestyle interventions leading to the plasma cholesterol changes as well as in the pharmacogenetics of statin treatment. On the contrary, APOA5 mutations detected in Czech population did not show strict effect on plasma TG levels. Val153 --> Met variant exhibit the sex-specific effect of HDL-cholesterol levels. The suggested roles of APOA5 variants in determination of the plasma remnant particles, plasma concentrations of C-reactive protein or some anthropometrical parameters were excluded.

• MeSH
• apolipoproteiny A genetika   krev   MeSH
• apolipoproteiny genetika   MeSH
• fenotyp MeSH
• financování organizované MeSH
• frekvence genu MeSH
• genetická predispozice k nemoci MeSH
• hodnocení rizik MeSH
• hypercholesterolemie genetika   krev   MeSH
• infarkt myokardu genetika   krev   prevence a kontrola   MeSH
• krysa rodu rattus MeSH
• lidé MeSH
• modely nemocí na zvířatech MeSH
• myši knockoutované MeSH
• myši MeSH
• polymorfismus genetický MeSH
• triglyceridy krev   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• lidé MeSH
• mužské pohlaví MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• přehledy MeSH

• Publikační typ
• abstrakty MeSH

• MeSH
• genotyp MeSH
• ghrelin * genetika   MeSH
• index tělesné hmotnosti * MeSH
• jednonukleotidový polymorfismus MeSH
• lidé MeSH
• obezita epidemiologie   genetika   MeSH
• poměr pasu a boků * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• dopisy MeSH
• komentáře MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Evropa MeSH

• Publikační typ
• abstrakty MeSH

BACKGROUND: Apolipoprotein A5 (APOA5) is a determinant of plasma lipids, and its role in body mass index (BMI) determination is discussed. This study was aimed at the investigation of the relationship between common APOA5 gene variants and body weight/plasma lipid decrease in overweight females. METHODS: We analyzed 98 unrelated overweight and obese nondiabetic Czech females (BMI >27.5). APOA5 T-1131-->C and Ser19-->Trp variants were genotyped. Before and after 9 weeks of lifestyle modification, biochemical and anthropometrical measurements and assessment of nutritional intake were performed. The lifestyle modification program consisted of a reduction in energy intake and an exercise program (aerobic exercise 4 times per week, 60 min each). RESULTS: The mean age of the participants was 30.7 +/- 3.7 years, the mean BMI before the intervention was 31.4 +/- 3.8 and the weight loss was 5.9 +/- 2.5 kg (7 +/- 3%). There were 86 T-1131T homozygotes and 12 carriers of the C-1131 allele and 82 Ser19Ser homozygotes and 16 carriers of the Trp19 allele, respectively; 72 females had the commonest T-1131T/Ser19Ser haplotype. No significant association between BMI decrease and APOA5 variants was found, but T-1131T carriers have a significantly higher body weight both before and after the intervention (p < 0.05; p = not significant for BMI). The fasting glycemia was significantly higher in Trp19 carriers both before and after the intervention (p < 0.01). Further, plasma triglyceride levels decreased in Ser19Ser homozygotes but increased in Trp19 carriers (1.42 +/- 0.62 to 1.28 +/- 0.48 vs. 1.15 +/- 0.47 to 1.41 +/- 0.80 mmol/l; p < 0.05 for differences between the groups). Similarly, in carriers of at least 1 less common APOA5 allele (n = 26), plasma low-density lipoprotein cholesterol levels did not decrease as they did in T-1131T/Ser19Ser carriers (3.11 +/- 0.70 to 3.27 +/- 0.81 vs. 3.39 +/- 0.81 to 3.16 +/- 0.86 mmol/l; p < 0.05 for differences between the groups). CONCLUSIONS: APOA5 gene variants have effects on the decrease in plasma triglyceride and low-density lipoprotein cholesterol level in females in a model combining their dietary habits and physical activity changes.

• MeSH
• analýza rozptylu MeSH
• apolipoproteiny A genetika   MeSH
• dospělí MeSH
• frekvence genu MeSH
• genetická variace MeSH
• genotyp MeSH
• hmotnostní úbytek fyziologie   MeSH
• index tělesné hmotnosti MeSH
• kohortové studie MeSH
• LDL-cholesterol krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• metabolismus lipidů genetika   MeSH
• nadváha krev   terapie   MeSH
• obezita krev   terapie   MeSH
• triglyceridy krev   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH