1. It will be determined whether polymorphisms in above mentioned genes participate in the genetic predisposition to lower weight loss after diet and physical intervention. 2. To characterize, in a Czech population, the frequencies of alleles in the genes for AHSG, receptor neuropeptide Y and preproorexin. To determine whether genetic variants correlate with BMI and/or WHR.

1.Studium polymorfizmů v genu pro varianty Met/Met v genu pro AHSG, v genu pro receptor neuropeptidu Y 585T>C:T a varianta v promotoru preproorexinu s variantou -909C>T rozšíří naše dosud ne příliš rozsáhlé znalosti o genetické determinaci tělesné hmotnosti. 2.Bude zjištěno, zda se varianty ve vyjmenovaných genech podílí na genetické predispozici k nižšímu poklesu váhy po intervenci dietou a fyzickou aktivitou. 3. Bude zjištěno, a to na rozsáhlém (2600 jedinců) homogenním reprezentativním vzorku české populace, zda varianty Met/Met v genu pro AHSG, v genu pro receptor neuropeptidu Y 585T>C:T a varianty v promotoru preproorexinu s variantou -909C>T, hrají roli v genetické determinaci tělesné hmotnosti .

• MeSH
• fetuin A MeSH
• genetická predispozice k nemoci MeSH
• kardiovaskulární nemoci prevence a kontrola   MeSH
• obezita patofyziologie   MeSH
• pohyb MeSH
• polymorfismus genetický MeSH
• pro-opiomelanokortin MeSH
• redukční dieta MeSH
• ženy MeSH

• Konspekt
• Biochemie. Molekulární biologie. Biofyzika
• NLK Obory
• vnitřní lékařství
• biologie
• kardiologie
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR

• Publikační typ
• abstrakty MeSH

OBJECTIVES: The AHSG (alpha2 Heremans-Schmid glycoprotein) gene is suggested to be important for the regulation of body fat and insulin sensitivity. Our study aimed to investigate the relationship between the common Thr248Met (rs4917) variant and obesity characteristics after an intervention with overweight females. MATERIAL AND METHODS: We analyzed 105 unrelated overweight/obese nondiabetic Czech adult females (49.0 ± 12.1 years, BMI over 28.6 kg/m2, mean BMI before intervention 32.8 ± 4.1 kg/m2) before and after 10 weeks of lifestyle modification. Biochemical and anthropometrical measurements were performed. The life style modification program consisted of a reduction of energy intake to an age-adjusted optimum and an exercise program (four units per week). The mean weight loss was 3.2 ± 3.5 kg (3.7 ± 4.0%). RESULTS: Thr/Thr homozygotes (N=52) increased lean muscle mass (p<0.05), decreased total body fat (kg) (p<0.005) and increased basal metabolic rate (BMR) per 1 kg body weight (p<0.005) in comparison with the Met carriers (N=52), but an association between BMI decrease and AHSG variants was not found. CONCLUSION: AHSG gene variants modify the effect of physical activity on BMR. Carriers of the Thr248Thr genotype showed a higher benefit from the lifestyle intervention (expressed as changes in body fat, active muscle and basal metabolic rate).

• MeSH
• adipozita genetika   MeSH
• cvičení MeSH
• dospělí MeSH
• energetický příjem MeSH
• fetuin A genetika   MeSH
• genetická variace genetika   MeSH
• genotyp MeSH
• heterozygot MeSH
• index tělesné hmotnosti MeSH
• inzulinová rezistence genetika   MeSH
• kosterní svaly anatomie a histologie   metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• metabolická clearance fyziologie   MeSH
• nadváha dietoterapie   genetika   metabolismus   MeSH
• obezita dietoterapie   genetika   metabolismus   MeSH
• obvod pasu MeSH
• polymerázová řetězová reakce MeSH
• poměr pasu a boků MeSH
• sedavý životní styl MeSH
• senioři MeSH
• složení těla fyziologie   MeSH
• životní styl MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

OBJECTIVES: Bias is common in the assessment of self-reported dietary intake and physical activity. Little is known about the reasons for this underreporting, which is obtained even from rural individuals and lean subjects. MATERIAL AND METHODS: Healthy volunteers (16 males, aged 18-55 years) were given two different full diets (high and low in saturated fat), each for a period of 4 weeks. RESULTS: The subjects were able to provide a report of their dietary intake with relatively high accuracy (68.4%) on day one. Accuracy declined on day two (64.2%), and on day three, the reporting accuracy was only 55.0% (p<0.05). Interestingly, on day one, individuals were more precise (p<0.05) in their dietary reports if they had consumed an unhealthy diet high in saturated fat (72.5%) than a healthy diet high in unsaturated fat (64.4%). The most frequently omitted items were fruits and vegetables. The non-reporting of food items consumed is very high, even in the short term, and individuals tend to remember unhealthy items better. CONCLUSIONS: Our pilot study showed that the inaccuracy of self-reported data includes not only the underestimation of energy intake (portion size) but also inaccurate qualitative data.

• MeSH
• dieta - přehledy normy   statistika a číselné údaje   MeSH
• dieta MeSH
• dospělí MeSH
• energetický příjem MeSH
• lidé středního věku MeSH
• lidé MeSH
• mastné kyseliny MeSH
• mladiství MeSH
• mladý dospělý MeSH
• ovoce MeSH
• přijímání potravy MeSH
• reprodukovatelnost výsledků MeSH
• venkovské obyvatelstvo MeSH
• zelenina MeSH
• zkreslení výsledků (epidemiologie) MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Variants within the FTO gene are important determinants of body mass index (BMI), but their role in determination of BMI changes after combined dietary/physical activity intervention is unclear. We have analyzed 107 unrelated overweight non-diabetic Czech females (BMI over 27.5 kg/m(2), age 49.2+/-12.3 years). FTO variants rs17817449 (first intron) and rs17818902 (third intron) were genotyped. The life style modification program (10 weeks) consisted of an age-matched reduction of energy intake and exercise program (aerobic exercise 4 times a week, 60 min each). The mean BMI before intervention was 32.8+/-4.2 kg/m(2) and the mean achieved weight loss was 4.8+/-3.5 kg (5.3+/-3.5 %, max. -15.5 kg, min. +2.0 kg, p<0.01). No significant association between BMI decrease and FTO variants was found. Also waist-to-hip ratio, body composition (body fat, water, active tissue), lipid parameters (total, LDL and HDL cholesterol, triglycerides) glucose and hsCRP changes were independent on FTO variants. FTO variants rs17817449 and rs17818902 are not associated with BMI changes after combined short time dietary/physical activity intervention in overweight females.

• MeSH
• dospělí MeSH
• financování organizované MeSH
• index tělesné hmotnosti MeSH
• LDL-cholesterol genetika   MeSH
• lidé středního věku MeSH
• lidé MeSH
• obezita genetika   MeSH
• polymorfismus genetický MeSH
• poměr pasu a boků MeSH
• proteiny genetika   MeSH
• složení těla MeSH
• triglyceridy genetika   MeSH
• tuková tkáň MeSH
• životní styl MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH

The INSIG2 (INSIG2 is primarily involved in the regulation of fatty acid and cholesterol synthesis) gene is suggested to be obesity related. An INSIG2 promoter variant, G-102A, has been detected and was demonstrated to be of potential functional significance. In two cohorts of middle-aged men, the association between this variant and BMI was suggested. We sought to replicate the association between the INSIG2 G-102A variant and BMI in three large Slavonic Caucasian populations. Further, we analysed the possible effect of this variant on BMI changes in a short-time intervention study. One thousand ninety-nine males and 1368 females (population- based Czech MONICA three-year cohort), 908 females from the 3PMFs study, together with 94 overweight (BMI > 27 kg/m2) females who underwent nine weeks of dietary/exercise intervention were genotyped for the INSIG2 G-102A variant using PCR-RFLP analysis. We could not detect any association between the INSIG2 G-102A variant and BMI or WHR with or without adjusting for age and gender in any population. Neither the BMI change nor anthropometric and lipid parameter changes were affected by the INSIG2 G-102A gene variant in intervened overweight females. However, MONICA females (but not males) carrying the common GG genotype had higher plasma levels of HDL cholesterol (GG homozygotes 1.51 ± 0.36 mmol/l vs. A allele carriers 1.45 ± 0.33; P < 0.05) in both surveys. Our results indicated that the G-102A INSIG2 polymorphism has no consistent effect on BMI in general populations, but could influence HDL cholesterol in females.

• MeSH
• alely MeSH
• běloši MeSH
• dospělí MeSH
• frekvence genu genetika   MeSH
• genetická predispozice k nemoci MeSH
• HDL-cholesterol genetika   MeSH
• index tělesné hmotnosti MeSH
• intracelulární signální peptidy a proteiny genetika   MeSH
• jednonukleotidový polymorfismus genetika   MeSH
• lidé středního věku MeSH
• lidé MeSH
• membránové proteiny genetika   MeSH
• promotorové oblasti (genetika) genetika   MeSH
• senioři MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH