Článek popisuje případ neúmyslného intravenózního podání prokain-penicilinu (P-PNC) místo ordinovaného krystalického penicilinu (G-PNC), přičemž tento výjimečný incident je analyzován z pohledu lidského faktoru a řízení kvality pomocí metody kořenové analýzy (Root Cause Analysis, RCA). Případ zdůrazňuje zásadní roli infuzního setu s filtrem, který zamezil průchodu větších částic suspenze do krevního oběhu pacienta a tím předešel vážnějším následkům. Incident je současně významným příkladem pro ošetřovatelskou praxi, který podtrhuje důležitost důsledného dodržování bezpečnostních a standardních operačních postupů (SOP) v rámci kompetencí sester a nutnost zapojení klinického farmaceuta při práci s LASA léčivy.
The article describes a case of unintended intravenous administration of procaine penicillin (P-PNC) instead of the prescribed crystalline penicillin (G-PNC). This unique incident is analyzed from the perspectives of human factors and quality management, using the Root Cause Analysis (RCA) method. A key element was the protective role of the infusion set with a filter, which prevented larger suspension particles from entering the patient's bloodstream, thereby averting more serious consequences. This case also serves as an important example for nursing practice, emphasizing the necessity of adhering to safety and Standard Operating Procedures (SOP) within the scope of nursing competencies, as well as the crucial role of clinical pharmacists in managing LASA (Look-Alike, Sound-Alike) medications.
- Keywords
- kořenová analýza, Hoigné syndrom,
- MeSH
- Administration, Intravenous MeSH
- Humans MeSH
- Medication Errors * MeSH
- Professional Competence MeSH
- Penicillin G Procaine administration & dosage adverse effects MeSH
- Quality Control MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Case Reports MeSH
- MeSH
- Adult MeSH
- Comorbidity MeSH
- Humans MeSH
- Breast Neoplasms complications MeSH
- Orbital Cellulitis * diagnosis etiology classification microbiology MeSH
- Penicillin G Procaine administration & dosage therapeutic use MeSH
- Staphylococcus aureus pathogenicity MeSH
- Streptococcus pyogenes pathogenicity MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Case Reports MeSH
Syphilis je systémová infekce s mnohočetnými projevy, které mohou napodobovat široké spektrum kožních i jiných nemocí. Důležitá je včasná diagnostika i vhodně zvolená antibiotická léčba.
Syphilis is a systemic infection with multiple manifestations that can mimic a wide range of skin and other diseases. Early diagnosis and appropriate antibiotic treatment are important.
- MeSH
- Penicillin G Benzathine administration & dosage therapeutic use MeSH
- Doxycycline administration & dosage therapeutic use MeSH
- Homosexuality, Male MeSH
- Middle Aged MeSH
- Humans MeSH
- Polymerase Chain Reaction MeSH
- Penicillin G Procaine administration & dosage therapeutic use MeSH
- Unsafe Sex MeSH
- Syphilis Serodiagnosis MeSH
- Sexually Transmitted Diseases diagnosis drug therapy MeSH
- Syphilis * diagnosis drug therapy MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Case Reports MeSH
Polymorphonuclear neutrophils (PMNs) play a key role in host defense. However, their massive accumulation at the site of inflammation can delay regenerative healing processes and can initiate pathological inflammatory processes. Thus, the efficient clearance of PMNs mediated by the induction of regulated cell death is a key process preventing the development of these pathological conditions. Myeloperoxidase (MPO), a highly abundant enzyme in PMN granules, primarily connected with PMN defense machinery, is suggested to play a role in PMN-regulated cell death. However, the contribution of MPO to the mechanisms of PMN cell death remains incompletely characterized. Herein, the process of the cell death of mouse PMNs induced by three different stimuli - phorbol 12-myristate 13-acetate (PMA), opsonized streptococcus (OST), and N-formyl-met-leu-phe (fMLP) - was investigated. MPO-deficient PMNs revealed a significantly decreased rate of cell death characterized by phosphatidylserine surface exposure and cell membrane permeabilization. An inhibitor of MPO activity, 4-aminobenzoic acid hydrazide, did not exhibit a significant effect on PMA-induced cell death compared to MPO deficiency. Interestingly, only the limited activation of markers related to apoptotic cell death was observed (e.g. caspase 8 activation, Bax expression) and they mostly did not correspond to phosphatidylserine surface exposure. Furthermore, a marker characterizing autophagy, cleavage of LC3 protein, as well as histone H3 citrullination and its surface expression was observed. Collectively, the data show the ability of MPO to modulate the life span of PMNs primarily through the potentiation of cell membrane permeabilization and phosphatidylserine surface exposure.
- MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Neutrophils metabolism pathology MeSH
- Peroxidase deficiency metabolism MeSH
- Regulated Cell Death MeSH
- Inflammation metabolism MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- MeSH
- Anti-Bacterial Agents administration & dosage therapeutic use MeSH
- Adult MeSH
- Humans MeSH
- Polymerase Chain Reaction MeSH
- Penicillin G Procaine administration & dosage therapeutic use MeSH
- Syphilis Serodiagnosis methods MeSH
- Sexually Transmitted Diseases diagnosis drug therapy MeSH
- Syphilis, Cutaneous diagnosis drug therapy MeSH
- Syphilis * diagnosis drug therapy MeSH
- Treponema pallidum MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Case Reports MeSH
The advantages of using mixtures of organic solvents for the separation of labeled oligosaccharides on the amide stationary phase under hydrophilic interaction liquid chromatography conditions are presented. The effect of the type of buffer as well as solvent or their mixtures on retention of uracil, saccharide labeling reagents (2-aminobenzoic acid, 2-aminobenzamide, ethyl 4-aminobenzoate, procainamide), and corresponding labeled saccharides were evaluated. The successful isocratic separation of labeled isomeric trisaccharides (maltotriose, panose, and isomaltotriose) was achieved in the mobile phase consisting of a 90% (v/v) mixture of organic solvents (methanol/acetonitrile 60:40) and 10% (v/v) 30 mM ammonium formate, pH 3.3. Changing the volume ratio between methanol/acetonitrile from 60:40 to 50:50 (v/v) allowed to obtain the separation of di-, tri-, and tetrasaccharides labeled by ethyl 4-aminobenzoate in less than 10.5 min.
- MeSH
- Acetonitriles chemistry MeSH
- Amides chemistry MeSH
- Chemistry Techniques, Analytical instrumentation methods MeSH
- Chromatography, Liquid * MeSH
- Formates chemistry MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Isomerism MeSH
- Oligosaccharides isolation & purification MeSH
- ortho-Aminobenzoates chemistry MeSH
- Solvents chemistry MeSH
- Carbohydrates chemistry MeSH
- Publication type
- Journal Article MeSH
An increasing resistance of human pathogenic bacteria and fungi has become a global health problem. Based on previous reports of 4-(salicylideneamino)benzoic acids, we designed, synthesised and evaluated their me-too analogues as potential antimicrobial agents. Forty imines derived from substituted salicylaldehydes and aminobenzoic acids, 4-aminobenzoic acid esters and 4-amino-N-phenylbenzamide were designed using molecular hybridization and prodrug strategies. The target compounds were synthesized with high yields and characterized by spectral methods. They were investigated against a panel of Gram-positive and Gram-negative bacteria, mycobacteria, yeasts and moulds. The most active imines were tested to determine their cytotoxicity and selectivity in HepG2 cells. Dihalogenosalicylaldehydes-based derivatives showed potent broad-spectrum antimicrobial properties, particularly against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (minimum inhibitory concentrations, MIC, from 7.81 µM) and Enterococcus faecalis (MIC of ≥15.62 µM), yeasts (MIC from 7.81 µM) and Trichophyton interdigitale mould (MIC of ≥3.90 µM). Methyl 4-[(2-hydroxy-3,5-diiodobenzylidene)amino]benzoate 4h exhibited excellent in vitro activity along with low toxicity to mammalian cells. This compound is selective for staphylococci, Candida spp. and Trichophyton interdigitale. In addition, this imine was evaluated as a potential inhibitor of Gram-positive biofilms. The successful approach used provided some promising derivatives with more advantageous properties than the parent 4-(salicylideneamino)benzoic acids.
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Antifungal Agents * pharmacology MeSH
- Arthrodermataceae MeSH
- Benzoates pharmacology MeSH
- Gram-Negative Bacteria MeSH
- Gram-Positive Bacteria MeSH
- Humans MeSH
- Methicillin-Resistant Staphylococcus aureus * MeSH
- Microbial Sensitivity Tests MeSH
- Molecular Structure MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
4-aminobenzoic acid (PABA), an essential nutrient for many human pathogens, but dispensable for humans, and its derivatives have exhibited various biological activities. In this study, we combined two pharmacophores using a molecular hybridization approach: this vitamin-like molecule and various aromatic aldehydes, including salicylaldehydes and 5-nitrofurfural, via imine bond in one-step reaction. Resulting Schiff bases were screened as potential antimicrobial and cytotoxic agents. The simple chemical modification of non-toxic PABA resulted in constitution of antibacterial activity including inhibition of methicillin-resistant Staphylococcus aureus (minimum inhibitory concentrations, MIC, from 15.62 µM), moderate antimycobacterial activity (MIC ≥ 62.5 µM) and potent broad-spectrum antifungal properties (MIC of ≥ 7.81 µM). Some of the Schiff bases also exhibited notable cytotoxicity for cancer HepG2 cell line (IC50 ≥ 15.0 µM). Regarding aldehyde used for the derivatization of PABA, it is possible to tune up the particular activities and obtain derivatives with promising bioactivities.
- MeSH
- Anti-Bacterial Agents chemistry pharmacology MeSH
- Hep G2 Cells MeSH
- Cytotoxins chemistry pharmacology MeSH
- 4-Aminobenzoic Acid chemistry pharmacology MeSH
- Folic Acid chemistry pharmacology MeSH
- Humans MeSH
- Methicillin-Resistant Staphylococcus aureus drug effects growth & development MeSH
- Microbial Sensitivity Tests MeSH
- Cell Survival drug effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
