Eukaryotic microorganisms
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The use of microorganisms as reducing and stabiliz-ing agents in biogenic syntheses of metal nanoparticles is an attractive approach. There is a large number of potential bioagents able to yield big amounts of various biomole-cules, and to prepare nanoparticles of diverse physico-chemical properties. Microscopic fungi and algae are widely studied for the preparation of nanoparticles, mainly because of their ability to produce vast amounts of extra-cellular proteins, enzymes, and other metabolites that can actively participate in the metal reduction and also contrib-ute to the nanoparticle stabilization. This results in highly stable metal nanoparticles with interesting properties that can be used, for example, as antimicrobial agents (especially Ag or Cu nanoparticles) or as catalysts. This review summarizes the main, promising representatives of microscopic fungi, yeasts, and algae used for the prepara-tion of nanoparticles of various metals.
Medical intelligence unit
200 s. : tab., grafy ; 24 cm
A new method providing a relief phase contrast for investigation of microorganisms by optical microscopy used a neutral filter Zeiss NG 10/1 that could be controllably slid at a certain azimuthal angle below the aperture condenser diaphragm of the microscope phase contrast. Two ways of application are described depending on the type of the microscope: (1) in a special holder, and (2) fixed on a rubber ring. The device enabled us to obtain excellent results in the area of both optical microscopy and microphotography. With the microorganisms visualized, a better resolution, higher contrast and a significant 3D effect were obtained; outer morphology and organelles (chloroplasts, nuclei, granules, oil reserve vacuoles, etc.) could also be investigated.
A new type of relief condenser mounted on a current laboratory microscope produced by Lambda Praha was used for the study of microorganisms of two kingdoms, Chromista and Plantae. The pictures obtained by the use of this device had a better resolving power and remarkable contrast, and a well visible 3D effect. Because of the absence of an aperture diaphragm the use was much simpler, compared to relief condensers whose construction was different.
... Contents in Brief -- UNIT ONE -- UNIT TWO -- UNIT THREE -- UNIT FOUR -- UNIT FIVE -- UNIT SIX -- MICROORGANISMS ... ... -- 2 Diversity of the Microbial World, 24 -- 3 Science of Microbiology: Methods for Studying Microorganisms ... ... GROWTH AND ITS CONTROL -- 9 Viral Replication, 256 -- 10 Bacterial Reproduction and Growth of Microorganisms ... ... , 286 -- 11 Control of Microbial Growth and Death, 310 -- MICROORGANISMS AND HUMAN DISEASES -- 12 Microorganisms ... ... the Urinary Tract, Genital Tract, Skin, Eyes, Ears, and Oral Cavity, 649 -- 23 Diseases Caused by Eukaryotic ...
XIX, 765 s. : il. ; 30 cm
- MeSH
- mikrobiologické jevy MeSH
- mikrobiologické techniky MeSH
- mikrobiologie MeSH
- Publikační typ
- atlasy MeSH
- Konspekt
- Mikrobiologie
- NLK Obory
- mikrobiologie, lékařská mikrobiologie
- infekční lékařství
Polarization microscopy has been used to study the internal structures of microbial cells and in terms of the birefringence of these structures and its possible relation to the cell function and composition. Cyanobacteria of the genus Phormidium were found to contain no anisotropic structures, while other microorganisms were found to contain them, albeit to a different extent, size, and number. The flagellate Euglena was found to contain two large anisotropic bodies, whereas the flagellate of the genus Phacus belonging to the same systematic group Euglenales was observed to contain only one large anisotropic body (storage substances--paramylon). On the other hand, green algae of the genus Scenedesmus, whose cells form four--celled coenobia, contained clusters of small anisotropic granules composed also of storage substances (volutin). Minute anisotropic granules (storage substances) in two smaller clusters were found also in diatoms of the genus Navicula, whereas the green alga of the genus Mougeotia was revealed to contain, in addition to minute anisotropic granules (storage substances) occurring in low numbers in the cytoplasm, also a strongly birefringent cell wall (shape birefringence). Cells of the amoeba of the genus Naegleria and heliozoans of the genus Heterophrys were observed to contain only isolated tiny anisotropic granules (storage substances).
