• Publikační typ
• zprávy MeSH

Precise localisation of brain lesion is critical for the outcome of epilepsy surgery. The definition of non-lesional epilepsy is dynamic, as new techniques are being developed and MRI scans with advanced imaging and post-processing methods can reveal lesions that were not evident previously. 80 healthy controls, 80/20 patients with pharmacoresistent epilepsy classified as non-lesional/lesional, resp., will be rescanned in the CEITEC Multimodal and Functional Imaging Laboratory (Siemens Prisma 3T, HD EEG 256 channels). Advanced functional and structural imaging methods will be combined to detect lesions: high density EEG; simultaneous HD EEG-fMRI; arterial spin labeling; advanced high-resolution structural imaging; diffusion tract and kurtosis imaging. We presume that subtle lesion will be defined by advanced multimodal imaging. The results will be validated by the further investigation of the newly detected lesion (i.e. SEEG), in operated patients by histology and long term outcome. Individual methods will be evaluated with the data acquired in patients with lesional epilepsies.

Přesná lokalizace mozkových lézí je zásadní pro chirurgii epilepsie. Definice nelezionální epilepsie je dynamická – stále se vyvíjejí zobrazovací techniky MRI a nové pokročilé metody zpracování signálu, které mohou objevit léze, jež nebyly zobrazitelné standardními metodami. 80 zdravých kontrol a 80/20 pacientů s farmakorezistentní epilepsií, diagnostikovanou jako nelezionální/lezionální v Centru pro Epilepsie Brno, bude vyšetřeno v CEITECu, Laboratoři Multimodálního a funkčního zobrazení (MR Siemens Prisma 3T, HD-EEG 256 kanálů). Budou kombinovány pokročilé techniky funkčního a strukturálního mapování mozku: HD EEG; simultánní HD EEG-fMRI; v MRI arterial spin labelling, pokročilé strukturální metody a zpracování, diffusion tract a kurtosis (DKI) zobrazení. Předpokládáme, že kombinace pokročilých metod umožní identifikovat subtilní léze, které předtím nebylo možné zobrazit. Nově objevené léze budou ověřeny klinicky (např. SEEG), u operovaných výsledky operace. Po vyhodnocení přínosu jednotlivých metod bude navržen algoritmus pokročilé diagnostiky u nelezionálních epilepsií.

• MeSH
• elektroencefalografie metody   MeSH
• magnetická rezonanční tomografie metody   MeSH
• mapování mozku metody   MeSH
• refrakterní epilepsie diagnostické zobrazování   MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• neurologie
• radiologie, nukleární medicína a zobrazovací metody
• NLK Publikační typ
• závěrečné zprávy o řešení grantu AZV MZ ČR

The number of reports on cognitive changes related to deep brain stimulation (DBS) surgery in Parkinson's disease (PD) is growing. The aim of the review was to assess whether high-density electroencephalography (HD EEG) in patients with DBS treatment for PD is used and mentioned as a research method for the assessment of cognitive function changes after DBS of the subthalamic nucleus. The HD EEG examination method demonstrates the effects of DBS on changes of connectivity among various areas of the brain using changes in bioelectric potentials recorded on the scalp during examination. The search results yielded seven studies on the possibility of using HD EEG to investigate changes in bioelectric potentials with various protocols (motor tasks, auditory stimuli). Based on the results, HD EEG appears to be a feasible research method in patients with PD and established DBS therapy. Therefore, we plan to use HD EEG to demonstrate the effects of DBS on changes of connectivity among various areas of the brain utilizing changes in bioelectric potentials recorded on the scalp during HD EEG examination.

• MeSH
• dospělí MeSH
• elektroencefalografie metody   MeSH
• hluboká mozková stimulace metody   MeSH
• kognitivní dysfunkce diagnóza   MeSH
• lidé středního věku MeSH
• lidé MeSH
• Parkinsonova nemoc diagnóza   patofyziologie   chirurgie   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• určení symptomu metody   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

The objective was to determine the optimal combination of multimodal imaging methods (IMs) for localizing the epileptogenic zone (EZ) in patients with MR-negative drug-resistant epilepsy. Data from 25 patients with MR-negative focal epilepsy (age 30 ± 10 years, 16M/9F) who underwent surgical resection of the EZ and from 110 healthy controls (age 31 ± 9 years; 56M/54F) were used to evaluate IMs based on 3T MRI, FDG-PET, HD-EEG, and SPECT. Patients with successful outcomes and/or positive histological findings were evaluated. From 38 IMs calculated per patient, 13 methods were selected by evaluating the mutual similarity of the methods and the accuracy of the EZ localization. The best results in postsurgical patients for EZ localization were found for ictal/ interictal SPECT (SISCOM), FDG-PET, arterial spin labeling (ASL), functional regional homogeneity (ReHo), gray matter volume (GMV), cortical thickness, HD electrical source imaging (ESI-HD), amplitude of low-frequency fluctuation (ALFF), diffusion tensor imaging, and kurtosis imaging. Combining IMs provides the method with the most accurate EZ identification in MR-negative epilepsy. The PET, SISCOM, and selected MRI-post-processing techniques are useful for EZ localization for surgical tailoring.

• MeSH
• dospělí MeSH
• elektroencefalografie MeSH
• epilepsie * diagnostické zobrazování   chirurgie   MeSH
• fluorodeoxyglukosa F18 * MeSH
• lidé MeSH
• magnetická rezonanční tomografie metody   MeSH
• mladý dospělý MeSH
• neurozobrazování metody   MeSH
• zobrazování difuzních tenzorů MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• Publikační typ
• časopisecké články MeSH

Parkinson's disease (PD) is a neurodegenerative condition with constantly increasing prevalence rates, affecting strongly life quality in terms of neuromotor and cognitive performance. PD symptoms include voice and speech alterations, known as hypokinetic dysarthria (HD). Unstable phonation is one of the manifestations of HD. Repetitive transcranial magnetic stimulation (rTMS) is a rehabilitative treatment thathas been shown to improve some motor and non-motor symptoms of persons with PD (PwP). This study analyzed the phonation functional behavior of 18 participants (13 males, 5 females) with PD diagnosis before (one pre-stimulus) and after (four post-stimulus) evaluation sessions of rTMS treatment, to assess the extent of changes in their phonation stability. Participants were randomized 1:1 to receive either rTMS or sham stimulation. Voice recordings of a sustained vowel [a:] taken immediately before and after the treatment, and at follow-up evaluation sessions (immediately after, at six, ten, and fourteen weeks after the baseline assessment) were processed by inverse filtering to estimate a biomechanical correlate of vocal fold tension. This estimate was further band-pass filtered into EEG-related frequency bands. Log-likelihood ratios (LLRs) between pre- and post-stimulus amplitude distributions of each frequency band showed significant differences in five cases actively stimulated. Seven cases submitted to the sham protocol did not show relevant improvements in phonation instability. Conversely, four active cases did not show phonation improvements, whereas two sham cases did. The study provides early preliminary insights into the capability of phonation quality assessment by monitoring neuromechanical activity from acoustic signals in frequency bands aligned with EEG ones.

• MeSH
• dysartrie MeSH
• elektroencefalografie MeSH
• fonace MeSH
• lidé MeSH
• Parkinsonova nemoc * MeSH
• pilotní projekty MeSH
• transkraniální magnetická stimulace metody   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• randomizované kontrolované studie MeSH

Stereoelectroencephalography (SEEG) records electrical brain activity with intracerebral electrodes. However, it has an inherently limited spatial coverage. Electrical source imaging (ESI) infers the position of the neural generators from the recorded electric potentials, and thus, could overcome this spatial undersampling problem. Here, we aimed to quantify the accuracy of SEEG ESI under clinical conditions. We measured the somatosensory evoked potential (SEP) in SEEG and in high-density EEG (HD-EEG) in 20 epilepsy surgery patients. To localize the source of the SEP, we employed standardized low resolution brain electromagnetic tomography (sLORETA) and equivalent current dipole (ECD) algorithms. Both sLORETA and ECD converged to similar solutions. Reflecting the large differences in the SEEG implantations, the localization error also varied in a wide range from 0.4 to 10 cm. The SEEG ESI localization error was linearly correlated with the distance from the putative neural source to the most activated contact. We show that it is possible to obtain reliable source reconstructions from SEEG under realistic clinical conditions, provided that the high signal fidelity recording contacts are sufficiently close to the source of the brain activity.

• MeSH
• elektroencefalografie metody   MeSH
• elektrokortikografie * metody   MeSH
• epilepsie * chirurgie   MeSH
• lidé MeSH
• magnetická rezonanční tomografie MeSH
• mapování mozku metody   MeSH
• neurozobrazování MeSH
• somatosenzorické evokované potenciály MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH