Q112367155
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Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
45 listů : il. ; 32 cm
ěádkovacím elektronovým mikroskopem bez zvláštních náročných úprav povrchu vzorků chceme přispět k lepšímu objektivnímu hodnocení tkání a materiálů v chirurgii.; By means of scanning electron microscope (SEM) of new generation we want to contribute to more objectivite evaluation of tissue surfaces and material surfaces in surgery.
BACKGROUND: The aim of this basic study was to investigate by scanning electron microscopy the effects of cytoskeleton inhibitors on conidiogenesis and capsule in the yeast Fellomyces fuzhouensis CBS 8243, related to Cryptococcus neoformans. METHODS: Cells were treated by methyl benzimidazole-2-ylcarbamate (BCM) and latrunculin A (LAT) in yeast extract peptone dextrose medium and examined by scanning electron microscopy. RESULTS: During conidiogenesis, mother cells covered by capsule formed hypha-like stalks and at the hyphal tip yeast-like conidium developed. LAT blocked both stages of conidiogenesis. Inhibited mother cells and conidia became spherical and their capsule disappeared. BCM did not block formation of conidia that were neckless, or affect capsule. Combined application of LAT and BCM blocked both stages of conidiogenesis, cells became spherical and their capsule disappeared. CONCLUSION: Yeast cells with disrupted actin cytoskeleton do not reproduce by conidiogenesis and do not retain inherited cell shape and capsule.
- MeSH
- Basidiomycota účinky léků ultrastruktura MeSH
- bicyklické sloučeniny heterocyklické farmakologie MeSH
- cytoskelet účinky léků ultrastruktura MeSH
- mikroskopie elektronová rastrovací metody MeSH
- spory hub účinky léků ultrastruktura MeSH
- thiazolidiny farmakologie MeSH
- tvar buňky účinky léků MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
