Cílem tohoto projektu je určit podíl E-kadherinu a cukerných složek plasmatické membrány pro agragaci nádorových buněk. Budou rovněž připraveny monoklonální protilátky specifické pro Lex glykosfingolipidy jaterních metastáz.; This project aims to determine the contribution of E-cadherin and carbohydrates of the plasma membrane to aggregation properties of tumor cells.Monoclonal antibodies specific for Lex GSL from liver metastases will also be prepared.

• MeSH
• agregace buněk patofyziologie   MeSH
• buněčná adheze MeSH
• kadheriny MeSH
• kultivované buňky MeSH
• monoklonální protilátky MeSH
• oligosacharidy MeSH

• Konspekt
• Obecná genetika. Obecná cytogenetika. Evoluce
• NLK Obory
• biologie
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR

Studies over the past decade have clearly shown that s.c. implant of primary and cultured tumor cells rarely leads to the occurrence of metastatic disease. Orthotopic transplantation of cell suspensions, surgical orthotopic implantation (SOI) of cancer tissue fragments resulted in metastases in many cancer types reaching 100% successful rate. We compared two metastatic models - heterotopic model of Lewis lung cancer and orthotopic B16 mouse melanoma. Both models were syngeneic with high metastatic ratio in C57BL/6 mice after transplantation of cancer cells, by injection into subcutaneous region of mice tail and without surgical intervention. The conclusion is that the localisation of cancer cell injection is a crucial condition for metastatic potential. The site with 100% haematogenous and lymph metastasis rate, after simple injection of cancer cells only, has been defined in mice, without dependence on the genetically predisposition and tumor cell line.

• MeSH
• karcinom plic Lewisové metabolismus   patologie   MeSH
• luminescentní proteiny metabolismus   MeSH
• melanom experimentální metabolismus   patologie   MeSH
• metastázy nádorů MeSH
• modely nemocí na zvířatech MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• zelené fluorescenční proteiny metabolismus   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

AIMS: The identification of growth factors and cytokines with angiogenic activity has enabled new therapeutic treatments for a variety of diseases; this concept is called therapeutic angiogenesis. The vascular endothelial growth factor (VEGF) is the most critical regulator of vascular formation. In the present study, we were interested in the therapeutic angiogenesis effect using plasmid transfer of human complementary DNA VEGF(165) (phVEGF(165)) in experimental skin and cartilage trauma. METHODS: Ten BALB/c mice were used for cartilage injury model. At 6 weeks of age, all mice were ear-punched, resulting in 2-mm-diameter puncture through the center of both pinnae. Each mouse got phVEGF(165) injection into the first ear and vector without insert or saline injection into the second one. The healing process was followed. The hollow diameter was measured on days 0, 14, and 42. Histological sections of experimental and control pinnae were taken from days 1, 3, 5, 7, 9, 11, 13, 15, 20, and 30 after experimental injury for hematoxylin and eosin and periodic acid Schiff staining and for human VEGF immunocytochemistry. The expression of human VEGF was also checked by real-time polymerase chain reaction in formalin-fixed, paraffin-embedded tissue sections. KEY FINDINGS: In BALB/c mouse strain, a significant angiogenesis promotion and cartilage repair were observed after phVEGF(165) injection into the punched ear area. SIGNIFICANCE: We suggest that administering phVEGF(165) leads to faster cartilage regeneration even if not only on the angiogenic basis.

• MeSH
• analýza rozptylu MeSH
• chrupavka zranění   MeSH
• fyziologická neovaskularizace účinky léků   MeSH
• hojení ran účinky léků   MeSH
• imunohistochemie MeSH
• kvantitativní polymerázová řetězová reakce MeSH
• modely nemocí na zvířatech MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• plazmidy MeSH
• regenerace MeSH
• studie proveditelnosti MeSH
• vaskulární endoteliální růstový faktor A farmakologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

We report a syngeneic model of spontaneous metastatic B16-F10 mouse melanoma in C57/BL6 mice with a very high metastatic frequency that mimics clinical metastatic melanoma. The B16 melanoma cells were injected between the skin and cartilage on the dorsal side of the ear. The model generated lymphatic and visceral metastases in all of the tested animals. In mice with large primary tumors, tumor weight correlated with the tumor growth time and also with the number of metastases in lymph nodes and organs. The dorsal ear space between the skin and cartilage enables both lymphatic and hematogenous metastatic spread. The model should be useful to study the mechanism of melanoma metastasis and to develop therapy for this currently untreatable disease.

• MeSH
• lymfatické metastázy MeSH
• melanom experimentální patologie   sekundární   MeSH
• modely nemocí na zvířatech MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• nádorové buněčné linie MeSH
• transplantace nádorů MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• Publikační typ
• abstrakty MeSH

• Publikační typ
• abstrakty MeSH

Tetraalkylammonium (TAA) derivatives have been reported to serve as stabilizers of asymmetrical cyanine dyes in aqueous solutions and to increase the yield and efficiency of polymerase chain reaction (PCR) detected by end-point analysis. In this study, we compared the ability of various TAA derivatives (with alkyl chain ranging from 1 to 5 carbons) and some other compounds to serve as enhancers of real-time PCR based on fluorescence detection from intercalating dye SYBR Green I (SGI). Our data indicate that TAA chlorides and some other TAA derivatives serve as potent enhancers of SGI-monitored real-time PCR. Optimal results were obtained with 10-16 mM tetrapropylammonium chloride. The effect of TAA compounds was dependent on the nature of counter ions present and composition of the reaction mixtures used. Based on measurements of SGI-generated fluorescence signal in the presence of PCR-amplified DNA fragments, oligonucleotide primers and/or various additives, we propose that TAA-derivatives reduce the binding of SGI to oligonucleotide primers and thus enhance primer-template interactions during annealing phase. Furthermore, these compounds serve as stabilizers of SGI-containing PCR mixtures. The combined data indicate that TAA derivatives might be a new class of additives contributing to robustness of real-time PCR monitored by asymmetrical cyanine dye SGI.

• MeSH
• financování organizované MeSH
• fluorescenční barviva analýza   MeSH
• kvartérní amoniové sloučeniny chemie   MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• organické látky analýza   MeSH
• polymerázová řetězová reakce metody   MeSH
• teplota MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• srovnávací studie MeSH

Many chemicals released into the environment can interfere with the action of the endocrine system through diverse mechanisms in animals and humans. This includes chemicals that occur naturally in plants such as phytoestrogens and also manmade chemicals (xenoestrogens). In our study we selected xenoestrogens (bisphenol-A (BPA), p-nonylphenol (NP), diethylstilbestrol (DES)) and phytoestrogens (genistein (GEN), resveratrol (RES)). All these pollutants represent of endocrine disruptors (ED). The effect of BPA, NP, DES, GEN and RES on the body weight, weight of different organs, sperm acrosomal status and in vivo fertility of CD1 mice was tested in a multigenerational study. The adult parental generation of mice and F1 and F2 generations were exposed to selected drugs for all their life. BPA and NP had a negative effect on offspring born of mice, on reproductive organs, and acrosome integrity of mice spermatozoa. Different effect was found in P, F1 and F2 generations. The fertility of mice exposed to DES was disrupted, especially in the first generation. Contrary to that, treatment by GEN and RES had no effect on fertility of CD1 mice but had some effect on reproductive parameters except of sperm integrity. Monoclonal antibodies against intra-acrosomal sperm proteins were used as biomarkers of sperm damage.

• Publikační typ
• abstrakty MeSH

The aim was to determine the effect of fybrinolytic therapy by streptokinase on chemotherapy and radiation response in human colon cancer cells. The cells were treated with different concentrations of gemcitabine, cis-platine and streptokinase, at a single use or in combinations. Radiation was tested at a dose 0.5, 5 and 15 Gy in three different schedules. The chemotherapy showed higher cytotoxic effect in combination with streptokinase. On the other hand, the combination of chemotherapy with streptokinase and radiotherapy provide no improvement in sensitivity of cancer cells to treatment. The data suggest that fybrinolytic therapy could influence the effect of chemotherapy.

• MeSH
• buňky HT-29 MeSH
• časové faktory MeSH
• cisplatina farmakologie   MeSH
• deoxycytidin analogy a deriváty   farmakologie   MeSH
• fibrinolytika farmakologie   MeSH
• financování organizované MeSH
• lidé MeSH
• nádory tračníku MeSH
• proliferace buněk účinky léků   účinky záření   MeSH
• protinádorové látky farmakologie   MeSH
• streptokinasa farmakologie   MeSH
• synergismus léků MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• srovnávací studie MeSH

• MeSH
• akrozom fyziologie   MeSH
• diethylstilbestrol aplikace a dávkování   MeSH
• finanční podpora výzkumu jako téma MeSH
• genistein aplikace a dávkování   MeSH
• spermatocyty fyziologie   ultrastruktura   MeSH
• tělesná hmotnost MeSH
• testis anatomie a histologie   MeSH
• testosteron krev   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH