Q80346865
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Congenital anomalies, diseases, and injuries may result in osteochondral damage. Recently, a big hope has been given to somatic stem cells (SSCs) which are characterized as undifferentiated cells with an ability of long-term self-renewing and plasticity. They are adherent with a fibroblast-like morphology in vitro and express various surface markers (e.g. CD29, CD73, CD90, and CD105), but they are negative for CD31, CD34, CD45, and HLA-DR. SSCs secrete various bioactive molecules, which are involved in processes of regeneration. The main goal of the present study was the characterization and comparison of biological properties of SSCs obtained from adipose tissue, dental pulp, and urine concerning osteochondral regeneration. SSCs were maintained in an appropriate growth medium up to the third passage and were analyzed by light and electron microscope. The immunophenotype was analyzed by flow cytometry. The kinetics of proliferation was measured by MTT assay. Human Cytokine/Chemokine Multiplex Assay was used, and SSCs secretory profile was measured by Luminex MAGPIX® Instrument. Pellet cultures and a chondrogenic medium were used to induce chondrogenic differentiation. Osteogenic differentiation was induced by the osteogenic medium. Chondrogenic and osteogenic differentiation was analyzed by real-time PCR. SSCs had similar fibroblast-like morphology. They have similar kinetics of proliferation. SSCs shared the expression CD29, CD44, CD73, CD90, and CD105. They lack expression of CD29 and CD34. SSCs secerned similar levels of IL10 and IL18 while differing in IFN-gamma, IL6, IL8, MCP-1, and RANTES production. SSCs possess a similar capacity for chondrogenic differentiation but slightly differ in osteogenic differentiation. In conclusion, it can be emphasized that SSCs from adipose tissue, dental pulp, and urine share the majority of cellular characteristics typical for SSCs and have great potential to be used in osteochondral tissue regeneration.
- MeSH
- buněčná diferenciace MeSH
- dospělé kmenové buňky * MeSH
- kultivované buňky MeSH
- lidé MeSH
- mezenchymální kmenové buňky * metabolismus MeSH
- osteogeneze MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Recently published studies suggest that the paracrine substances released by mesenchymal stem cells (MSCs) are the primary motive behind the therapeutic action reported in these cells. Pre-clinical and clinical research on MSCs has produced promising outcomes. Furthermore, these cells are generally safe for therapeutic use and may be extracted from a variety of anatomical regions. Recent research has indicated, however, that transplanted cells do not live long and that the advantages of MSC treatment may be attributable to the large diversity of bioactive substances they create, which play a crucial role in the control of essential physiological processes. Secretome derivatives, such as conditioned media or exosomes, may provide significant benefits over cells in terms of manufacture, preservation, handling, longevity of the product, and potential as a ready-to-use biologic product. Despite their immunophenotypic similarities, the secretome of MSCs appears to vary greatly depending on the host's age and the niches in which the cells live. The secretome's effect on multiple biological processes such as angiogenesis, neurogenesis, tissue repair, immunomodulation, wound healing, anti-fibrotic, and anti-tumor for tissue maintenance and regeneration has been discovered. Defining the secretome of cultured cultivated MSC populations by conditioned media analysis will allow us to assess its potential as a novel treatment approach. This review will concentrate on accumulating data from pre-clinical and clinical trials pointing to the therapeutic value of the conditioned medium. At last, the necessity of characterizing the conditioned medium for determining its potential for cell-free treatment therapy will be emphasized in this study.
This article summarizes the importance of the exact morphology of human uterine/fallopian tube epithelium at the scanning electron microscopy (SEM) level for the clinical outcome even nowadays. Visual referential micrographs from SEM reflect two ways to view human epithelial cell lining surfaces: the surface epithelial uterine tube from surgical tissue biopsy and human fallopian tube epithelial cells (HFTEC) culture monolayer surface. One colorized image visualizes ciliated cells, distinguishes them from non-ciliated cells, and provides an educational benefit. A detailed description of the ultrastructure in referential and pathologic human uterine tube epithelium is important in defining the morphological basis of high-grade carcinomas, in the mechanism of pathophysiology, and in discussing options for its prevention. Cell cultures of human fallopian tube epithelial cells offer new approaches in simulating the mechanisms of cancer genesis or may help to elucidate the genetic basis of several diagnoses. New technical approaches in SEM provide higher resolution and detailed surface images. The SEM modality is still one of the current options in diagnostics and may be useful for advancing human reproductive organ cancer research.
- MeSH
- biopsie MeSH
- buněčné kultury MeSH
- elektrony * MeSH
- epitel MeSH
- epitelové buňky MeSH
- lidé MeSH
- mikroskopie elektronová rastrovací MeSH
- vejcovody * patologie fyziologie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Despite significant advances in medical research, plastic surgeons still face a shortage of suitable patient tissues, and soft tissue reconstruction is no exception. In recent years, there has been a rapid boom in the use of acellular dermal matrix (ADM) in reconstructive and aesthetic surgery. ADM is incorporated into the surrounding tissue and gradually replaced by the host's collagen, thus promoting and supporting the healing process and reducing the formation of scar tissue. The main goal of this article is to provide a brief review of the current literature assessing the clinical applications of ADM across a broad spectrum of applications in plastic and reconstructive surgery.
- MeSH
- acelulární dermis * MeSH
- hojení ran MeSH
- lidé MeSH
- plastická chirurgie * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Hojenie je proces do ktorého sú zapojené rôzne bunky a komponenty extracellulárnej matrix, ktoré vzájomne medzi sebou neustále komunikujú a pôsobia súčastne smerom ku spoločnému cietu. Larvy najčastejšie používanej bzučivky Lucilia (Phaenicia) sericata, alebo tzv greenbottie sa aplikujú do chronických rán za účelom zlepšenia hojenia, ked konvenčná liečba zlyhala. Magott terapia (MDT, larválna terapia, biochirurgická liečba) má nasledujúce 3 prospešné efekty na ranu: očistenie, dezinfekciu a zlepšenie hojenia. Po aplikácii lariev do nekrotickej rany zlepšia hojenie vdaka kombinácii exkretorických/sekretorických (ES) proteáz, ktoré sú zapojene do remodelácie komponentov extracelulárnej matrix (ECM). Larválne ES sú príčinou zmien v adhézii íibroblastov a ich šírení po proteínových povrchoch komponentov extracelulárnej matrix a môžu zasiahnuť do integrity proteínových povrchov, najmä fibronektínu, zatiaľ čo životaschopnost buniek ostane zachovaná.
Wound healing is a collaborative process involving a variety of cells and matrix components which need to interact continually towards a common goal. Lucilia (Phaenicia) sericata larvae, or green bottle fly maggots are applied to chronic wounds to aid healing when conventional "treatments have failed. Maggot therapy (MDT, larval therapy, biochirurgic therapy) has the following three beneficial effects on a wound: debridement, disinfection and enhanced healing. When maggots are introduced into necrotic wound, they potentially influence wound healing events with combination of excretory/ secretory (ES) proteases which are involved in the remodeling of extracellular matrix (ECM) components. Magott ES caused changes in fibroblast adhesion and spreading upon ECM protein surfaces and affected the integrity of the protein surfaces, especially fibronectin, whilst maintaining cell viability.
- Klíčová slova
- Lucilia sericata,
- MeSH
- biologická terapie metody MeSH
- Diptera MeSH
- fibroblasty MeSH
- financování organizované MeSH
- hmyz růst a vývoj MeSH
- hojení ran fyziologie MeSH
- infekce v ráně terapie MeSH
- larva enzymologie MeSH
- lidé MeSH
- nekróza terapie MeSH
- proteasy terapeutické užití MeSH
- Check Tag
- lidé MeSH
RAG belongs to appropriate inhibitors of protein glycation, i.e. formation of advanced glycation end products, which are thought to be responsible for some complications of DM, including neuropathy, angiopathy, retinopathy and nephropathy. In the present study authors have evaluated the genotoxic effect of RAG on the cell culture of human neonatal fibroblasts (B-HNF-1) in regard to its potential clinical application as inhibitor of advanced glycation end products in relationships to the pathogenesis of chronic diabetic complications. The direct contact cytotoxicity assay and micronucleus test were performed. The results showed that RAG in the concentration range of 1 x 10-4 to 1 x 10-6 mol.l-1 did not induce any changes in the morphology of exposed B-HNF-1 cells. The frequency of micronuclei was not significantly increased as well. The inhibitive effect of resorcylidene aminoguanidine was directly proportional to its concentration. It can be concluded that RAG at the selected concentrations has an inhibitive effect on proliferation of the treated cells and, at the same time, does not display any genotoxic effects on B-HNF-1 cells.
- MeSH
- buněčné linie MeSH
- cytotoxiny farmakologie chemie MeSH
- diploidie MeSH
- fibroblasty cytologie fyziologie účinky léků MeSH
- guanidiny farmakologie chemie toxicita MeSH
- kojenec MeSH
- lidé MeSH
- mikrojaderné testy MeSH
- molekulární struktura MeSH
- mutageny farmakologie chemie MeSH
- tvar buňky účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- Check Tag
- kojenec MeSH
- lidé MeSH
- mužské pohlaví MeSH
Background: Skeletal muscle contains populations of multipotent adult stem cells also referred to as muscle–derivedstem cells.Aim: The main goal of this study was to isolate and culture human adult stem cells from skeletal muscle and characterizethem.Methods: Muscle–derived stem cells were isolated from biopsy specimens of femoral muscle. The cells werecultured in Dulbecco's modifi ed Eagle's minimal essential medium supplemented with 10% fetal calf serum and gentamycin.When they reached confl uence, they were sub–passaged up to the third passage. Cells from the last passagewere prepared for TEM analysis. Production of ?–actin and desmin was confi rmed by histochemistry. Moreover, thephenotypic characterization was performed.Results: Primary isolated muscle–derived stem cells had a fi broblast–like shape. During subsequent passages theymaintained this morphology. TEM analysis showed typical ultrastructural morphology of mesenchymal stem cells.They had large pale nuclei with a large amount of euchromatine. Nuclei were irregular with noticeable nucleoli. Dilatedcisterns of rough endoplasmic reticulum were present in cytoplasm. In certain parts of the cytoplasm there were aggregatesof granules of glycogen. The products of cells were actively secreted into the extracellular matrix. They expressed?–actin and desmin. The results of phenotypic characterization showed that almost all analyzed cells were CD13,CD34, CD56 positive and CD45 negative. Moreover, they did not express anti–human fi broblast surface protein.Conclusions: Muscle–derived stem cells exhibited typical characteristics typical for mesenchymal stem cells. Afteranalysis of their diff erentiation potential they could be used in tissue engineering and regenerative medicine.
- MeSH
- biopsie metody využití MeSH
- dediferenciace buněk MeSH
- financování organizované MeSH
- fluorescenční protilátková technika metody využití MeSH
- gentamiciny MeSH
- imunohistochemie metody využití MeSH
- kultivační techniky metody využití MeSH
- lidé MeSH
- mezenchymální kmenové buňky cytologie MeSH
- multipotentní kmenové buňky cytologie MeSH
- regenerativní lékařství metody trendy MeSH
- svalové buňky účinky léků MeSH
- tkáňové inženýrství metody trendy MeSH
- Check Tag
- lidé MeSH
- MeSH
- bloudivá slezina diagnóza etiologie patologie MeSH
- embryo savčí abnormality anatomie a histologie MeSH
- embryonální a fetální vývoj * fyziologie genetika MeSH
- embryonální struktury abnormality anatomie a histologie MeSH
- gonády abnormality anatomie a histologie patologie MeSH
- heterotaxe diagnóza patologie MeSH
- lidé MeSH
- mikrofotografie metody využití MeSH
- slezina * anatomie a histologie embryologie růst a vývoj MeSH
- statistika jako téma MeSH
- vrozené vady * klasifikace patofyziologie patologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
Športové a technické potápanie sa stali v ostatných dvadsiatich rokoch nezvyčajne populárnymi. Dovtedy bolo potápanie len prioritou polície, vojska a vedcov. Dnes sa stále viac a viac ľudí vystavuje podmienkam abnormálne zvýšeného okolitého tlaku. To nesie so sebou určité riziká. K najvážnejším patrí dekompresná choroba. Aj napriek najmodernejším výskumom, nie je mechanizmus dekompresnej choroby vo všetkých jej parametroch objasnený. Väčšina experimentov sa orientuje na simulovanie dekompresie v živých organizmoch. Zväčša dochádza k ich úmrtiu. Zásadným novým prístupom v predkladanej práci je použitie pracovného média in vitro ako prostriedku na vizualizáciu dekompresných zmien. Cieľom predkladanej práce je objasnenie možnosti a vhodnosti využitia tkanivových kultúr na simulovanie dekompresie in vitro, vypracovanie spôsobu vizualizácie a hodnotenia plynových bublín vzniknutých in vitro, simulovanie tvorby bublín pri reálnom dekompresnom ponore s použitím vzduchu ako kompresnej a izokompresnej plynovej zmesi a s použitím EANx50 ako dekompresnej plynovej zmesi. Ako simulované médium sme si zvolili štandardizované tkanivové kultúry z ľudských fibroblastov B-HEF-2 a tkanivové kultúry z neuroblastových buniek N2A myší. Dekompresiu sme rozdelili do dvoch profilov. „Explozívny“ dekompresný profil s lineárnou kompresiou do vysokého tlaku (50 bar) a náhlou dekompresiou na atmosférický tlak (1 bar). Reálny dekompresný profil predstavuje ponor s použitím vzduchu ako kompresného a izokompresného plynu a EANx50 ako dekompresného plynu. Naplánovali sme ho s použitím VPM-B modelu dekompresie softvérom V-Planner v.3.62. Naše najnovšie experimentálne štúdie poukazujú na možnosť fragmentácie DNA a aktiváciu apoptózy po dekompresnom šoku. Využitie tkanivových kultúr sa v tomto prípade javí ako veľmi vhodné pre ďalšie štúdium dekompresných zmien na celulárnej a subcelulárnej úrovni.
Recreational and technical diving in the past twenty years have become extraordinary popular. Until then diving was a priority of police, army and scientists. Today more and more enthusiasts are exposed to abnormally high surrounding pressure. To the severest of all risks associated with diving, decompression sickness is the greatest threat. Even the most advanced scientific approaches yet did not reveal the complex mechanisms of decompression sickness formation. The majority of experiments use a live model for decompression sickness studies. This is associated with high fatality rate. The new approach of this scientific study is in the use of an in vitro working media as a means of visualization of gas bubbles due to decompression. The aim of this study is to bring above new approaches in in vitro gas bubble formation during decompression. Main goals are: development of in vitro inert gas bubble visualization and evaluation techniques after a real decompression dive with air a as the diving gas and EANx50 as a decompression gas. We selected cell cultures derived from human fibroblasts B-HEF-2 and cell cultures of mice N2A neuroblasts. We used two decompression profiles. “Explosive” decompression profile with a linear compression to overpressure of 50 bar with an rapid decompression to atmospheric pressure (1 bar). In simulation of a realistic decompression profile we used compressed air as a mean for compression and isocompression breathing gas and an EANx50 blend as a decompression breathing gas. This dive was planned with the use of a VPM-B decompression profile by V-Planner software v.3.62. Our latest experiments show that DNA fragmentation and apoptosis is activated during decompression shock. Use of culture cells can be of great value in further studies of decompression changes in the cellular and subcellular level.
Congenital abnormalities, various diseases and injuries may result in the degeneration of articular cartilage. Recently, stem cell therapy has offered new treatment possibilities for this condition. The aim of our study was to verify the chondrogenic differentiation potential of human bone marrow mesenchymal stem cells (BMSCs) and adipose tissue-derived mesenchymal stem cells (AMSCs) in vitro in the presence or absence of transforming growth factor beta (TGF-beta1). Human BMSCs and AMSCs from healthy donors were collected during orthopaedic surgeries and expanded in vitro to obtain a sufficient quantity of cells; their chondrogenic differentiation was studied in the pellet culture system. Spontaneous chondrogenesis occurred in both BMSC and AMSC pellet cultures and was similar in both TGF-beta1 treated and untreated pellet cultures. BMSC pellets contained more cells with a chondrogenic phenotype. The presence of TGF-beta1 led to a decrease in the levels of collagen type I mRNA and to increased levels of collagen type II mRNA only in the BMSC pellet culture. Our results demonstrate that although both mesenchymal cell types can be used in cartilage tissue engineering, the chondrogenic potential of human BMSCs is higher than that of AMSCs.
- MeSH
- finanční podpora výzkumu jako téma MeSH
- imunohistochemie metody využití MeSH
- kloubní chrupavka abnormality patologie růst a vývoj MeSH
- kolagen typ II fyziologie genetika MeSH
- kostní dřeň fyziologie chirurgie MeSH
- kultivované buňky fyziologie transplantace MeSH
- lidé MeSH
- mezenchymální kmenové buňky fyziologie MeSH
- polymerázová řetězová reakce s reverzní transkripcí metody využití MeSH
- transformující růstový faktor beta1 terapeutické užití MeSH
- tuková tkáň fyziologie chirurgie MeSH
- Check Tag
- lidé MeSH
