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second edition xiv, 431 stran : ilustrace ; 24 cm
100 rats were randomly divided into a sham-operated group and middle cerebral artery occlusion (MCAO) modeling groups. The sham group after surgery was observed for 14 days. After MCAO, some rats received isometric contraction training (ICT) which was as follows: an atraumatic tourniquet was placed around left or right hind limb to achieve hind limb ischemia for 5 min, followed by 5 min of reperfusion, 4 cycles for one time, once a day, and five days per week. The MCAO modeling groups included the following four groups: i) a group only received MCAO, and was observed for seven days (MCAO-7d), ii) a group only received MCAO, and was observed for 14 days (MCAO-14d), iii) a group, after MCAO, received ICT for seven days (ICT-7d), and iv) a group, after MCAO, received ICT for 14 days (ICT-14d). Brain infarct area, behavioral outcomes, the number of neurons, apoptosis, cerebral edema and cerebral water content were assessed, respectively. The mRNA expression of vascular endothelial growth factor (VEGF) was assayed with RT-PCR, and protein expression of VEGF was quantified with western blot. compared with MCAO controls, cerebral infarction, neurological deficits and neuronal apoptosis were reduced significantly in the ICT groups, while the number of neurons was increased. Moreover, the mRNA expression of VEGF and protein expression of VEGF were enhanced after 1 and 2 weeks of ICT. ICT may promote angiogenesis and neuroprotection after ischemic stroke and this new remodeling method provide a novel strategy for rehabilitation of stroke patients.
- MeSH
- cévní mozková příhoda * terapie MeSH
- infarkt arteria cerebri media MeSH
- ischemie mozku * metabolismus MeSH
- isometrická kontrakce * MeSH
- kondiční příprava zvířat * MeSH
- krysa rodu rattus MeSH
- messenger RNA MeSH
- modely nemocí na zvířatech MeSH
- neuroprotekce MeSH
- potkani Sprague-Dawley MeSH
- vaskulární endoteliální růstový faktor A metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- hypotyreóza * epidemiologie krev prevence a kontrola MeSH
- jod * aplikace a dávkování moč nedostatek MeSH
- lidé MeSH
- referenční hodnoty MeSH
- štítná žláza ultrasonografie MeSH
- thyreotropin * analýza krev účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- souhrny MeSH
- Geografické názvy
- Čína MeSH
The present study was designed to examine effects of Sinomenine (SM) on glioma cells growth in vivo and in vitro. Cells growth and apoptosis were detected by MTT assay, TUNEL assay and flow cytometric analysis. In the study, SM treatment led to growth inhibition on a series of glioma cell lines, including U87, U373, U251, Hs683 and T98G. SM prevented U87 growth in the nude mice as well. Inhibitory effects of SM on U87 cells proliferation in vitro and in vivo were more effective than that of temozolomide (TMZ), and SM has synergistic effects with TMZ in the glioma therapy. SM induced apoptotic death in U87 cells via activation of caspase-3, caspase-8 and caspase-9, and down-regulation of HIAP, Bcl-2 and survivin. Moreover, we observed SM decreased the expression of phosphorylated STAT3 (p-STAT3) both in vivo and in vitro. Interestingly, using a specific activator of STAT3, we demonstrated overexpression of p-STAT3 impaired, SM mediated growth inhibition and apoptosis induction in the U87 cells. In summary, our results indicate SM induced growth suppression of human glioma cells through inhibiting phosphorylation of STAT3.
- MeSH
- alkaloidy farmakologie terapeutické užití MeSH
- apoptóza genetika imunologie účinky léků MeSH
- astrocyty účinky léků MeSH
- gliom farmakoterapie patologie ultrastruktura MeSH
- kaspasy analýza metabolismus MeSH
- kultivované buňky MeSH
- nádorové buněčné linie imunologie metabolismus účinky léků MeSH
- signální transdukce MeSH
- Sinomenium chemie MeSH
- techniky in vitro MeSH
- transkripční faktor STAT3 antagonisté a inhibitory metabolismus účinky léků MeSH
To study the effect of sinomenine (Sin) on isoproterenol (Iso, β-agonist)-induced cardiac hypertrophy (CH), we set up four mouse groups: control, Iso model, Iso+metoprolol (Met, β blocker) 60 mg/kg and Iso+Sin 120 mg/kg. CH was induced by Iso (s.c. for 28 days) in mice, and Sin or Met were orally administered by gavage for 28 days in total. Left ventricular diastolic anterior wall thickness (LVAWd), left ventricular diastolic posterior wall thickness (LVPWd), left ventricular ejection fraction (LVEF), and short axis shortening (FS) were measured by echocardiography. Malondialdehyde (MDA) and total superoxide dismutase (T-SOD) were measured by commercial kits. Lactate dehydrogenase (LDH), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β) were measured by ELISA kits. Histological changes were observed using hematoxylin-eosin (HE) and Masson staining. Protein level of nuclear transcription factor-kappa B (NF-κB) was detected by immunohistochemistry. Compared with the control group, LVAWd, Left ventricular weight index (LVWI) and myocardial fibrosis of the Iso model group significantly increased, as well as NF-κB, LDH, MDA, TNF-α, and IL-1β levels. However, the activity of T-SOD decreased. Compared with the Iso model group, LVWI of Iso model+Sin or Iso model+Met group was improved, LVAWd, LVPWd and myocardial fibrosis decreased, and NF-κB, LDH, MDA, TNF-α and IL-1β levels decreased. T-SOD activity also increased. This study reveals that Sin inhibits the activation of NF-κB, lowers the levels of TNF-α and IL-1β, has anti-oxidative stress effect and inhibits myocardial inflammation in mouse heart, thereby demonstrating its efficacy in preventing Iso induced CH.
- Klíčová slova
- Argentum nitricum,
- MeSH
- Gelsemium MeSH
- homeopatie * metody MeSH
- lidé MeSH
- průjem terapie MeSH
- virové nemoci terapie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- MeSH
- draslíkové kanály fyziologie účinky léků MeSH
- hypoxie patofyziologie MeSH
- krysa rodu rattus MeSH
- plíce krevní zásobení MeSH
- synthasa oxidu dusnatého antagonisté a inhibitory fyziologie MeSH
- vazodilatace fyziologie účinky léků MeSH
- vazokonstrikce fyziologie účinky léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
To resolve the problem of the insufficient availability of seed cells and to provide seed cells for tissue engineering research, an immortalized human bone marrow stromal stem cell line (MSCxj cells) was established in our department to investigate the ectopic osteogenesis of MSCxj cells. MSCxjs were grown with a heterogeneous bone scaffold for 48 h. Three groups were included: A: MSCxjs of 35 PDs were maintained with heterogeneous bone; B: MSCxjs of 128 PDs were maintained with heterogeneous bone; and C: heterogeneous bone alone. Tetracycline fluorescence staining, H&E staining, and ponceau staining, immunohistochemistry and bone histomorphometry were performed. At the same time, scanning electron microscopy was conducted to detect the growth of MSCxjs and heterogeneous bone. Scanning electron microscopy showed favorable adherence of MSCxjs to heterogeneous bone. A large number of newly generated filamentous extracellular matrix and fine granular materials were found to cover the cells. The results from staining showed that the osteogenesis was not obvious in group A/B 4 weeks after transplantation. Eight weeks after implantation, osteoid matrix deposition was noted in and around the heterogeneous bone in group A/B. Twelve weeks after implantation, osteogenesis was increased in group A/B. There were no significant differences in the time course for bone formation and the amount of newly generated bone between group A/B. Like primary hBMSCs, MSCxj cells have favourable ectopic osteogenesis and can be applied as seeded cells in bone tissue engineering.
- MeSH
- experimenty na zvířatech MeSH
- kmenové buňky cytologie enzymologie MeSH
- kostní dřeň MeSH
- lidé MeSH
- myši MeSH
- osteogeneze MeSH
- statistika jako téma MeSH
- techniky tkáňových kultur metody využití MeSH
- tkáňové inženýrství metody trendy využití MeSH
- transplantace heterologní metody využití MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- Publikační typ
- přehledy MeSH
