• MeSH
• lidé MeSH
• melaniny genetika   MeSH
• nemoci očních víček patologie   MeSH
• poruchy pigmentace patologie   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH

• MeSH
• elektronová mikroskopie MeSH
• lidé středního věku MeSH
• melaniny analýza   MeSH
• melanom ultrastruktura   MeSH
• sarkom ultrastruktura   MeSH
• Check Tag
• lidé středního věku MeSH
• mužské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH

• MeSH
• cholelitiáza etiologie   MeSH
• melaniny metabolismus   MeSH
• melanom diagnóza   MeSH
• pigmentace kůže MeSH
• Publikační typ
• přehledy MeSH

• MeSH
• melaniny fyziologie   MeSH

• MeSH
• kůže MeSH
• melaniny fyziologie   MeSH

• MeSH
• autoradiografie MeSH
• cytologické techniky MeSH
• melaniny MeSH

• Konspekt
• Fyzioterapie. Psychoterapie. Alternativní lékařství
• NLK Obory
• radiologie, nukleární medicína a zobrazovací metody
• histologie

The authors demonstrate on the case of a 6-year-old girl with systemic mastocytosis (with infiltration of the bone marrow and hepatopathy) and urticaria pigmentosa the activation of the epidermal melanin unit. In the biopsy of the skin lesion they observed numerous Langerhans cells (CD1a), an increased proliferating activity of cells of the str. basale and adjacent cells of the str. spinosum (PC-10) and a positive finding of protein p53. Stimulation of melanogenesis was suggested by melanin deposits in the cells of the epidermal melanin unit (Warthin-Starry, Fontána) which correlated with the tyrosinase activity (L-DOPA reaction) which was observed also in individual melanocytes diffusely dispersed in the upper and median dermis. On the presented case the authors demonstrate the necessity of long-term follow-up of these patients focused on possible neo processes of the skin incl. malignant melanoma.

• MeSH
• dítě MeSH
• keratinocyty ultrastruktura   MeSH
• Langerhansovy buňky ultrastruktura   MeSH
• lidé MeSH
• mastocytóza komplikace   patologie   MeSH
• melanocyty ultrastruktura   MeSH
• urticaria pigmentosa MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH

BACKGROUND: One of the pathogenic mechanisms of the progression non-alcoholic liver disease (NAFLD) to nonalcoholic steatohepatitis (NASH) is the accumulation of reactive oxygen species (ROS). So, antioxidant therapy is necessary for successful treatment of the liver injury. We have paid attention to melanin produced by yeast Nadsoniella nigra strain X-1 as novel antioxidant and anti-inflammatory agents with low toxicity. In current study we aimed to investigate the preventive effect of melanin on the monosodium glutamate (MSG) induced NAFLD model in rats. METHODS: The study was carried out on 45 Wistar rats that were divided into 3 groups: intact, MSG- and MSG+melanin groups (n=15 in each group). Newborn rats of MSG- and MSG+melanin groups were administered with MSG (4mg/g, 8μl/g, subcutaneously) at 2nd-10th days of life. Since the age of 1 month, rats of MSG-group were treated with water (0.25ml/100g), rats of MSG+melanin groups-with melanin (1mg/kg) dissolved in water (0.25ml/100g). INTRODUCTION: had been performed intermittently (two-week courses alternated with two-week breaks) for 3 months. In 4-month rats anthropometrical parameters and visceral adipose tissue (VAT) mass were estimated. To assess morphological changes in liver we used NAS (NAFLD activity score). The content of pro-inflammatory cytokines (interleukin (IL)-1β, IL-12Bp40, interferon (INF)-γ) and anti-inflammatory cytokines (IL-4, IL-10, tumor growth factor (TGF)-β) were measured by ELISA. RESULTS: We found significantly lower total score (1.0±0.19 vs 3.33±0.36, p<0.001), degree of steatosis (0.73±0.18 vs 1.80±0.17, p<0.001) and manifestation of lobular inflammation (0.27±0.11 vs 1.20±0.17, p<0.001) due to NAFLD activity score in MSG+melanin group compared to MSG-obesity. NASH we confirmed only in 33.3% of rats with MSG-obesity that was significantly higher than after melanin (6.7%) administration (p=0.033). Melanin administration reduce amount of visceral fat on 44.5% (p<0.001) as compared to MSG-obesity group. Melanin reduced the content of IL-1β in rat serum and restored the level of anti-inflammatory cytokines (IL-10, TGF-β) to the control values. CONCLUSION: Thus, the administration of melanin can prevent development of NAFLD/NASH in rats with MSG-induced obesity and can be considered as possible novel therapeutic agents but further studies to confirm its action needed.

• MeSH
• abdominální obezita chemicky indukované   prevence a kontrola   MeSH
• adipokiny metabolismus   MeSH
• antioxidancia farmakologie   MeSH
• cytokiny metabolismus   MeSH
• glutamát sodný MeSH
• játra patologie   MeSH
• krysa rodu rattus MeSH
• melaniny farmakologie   MeSH
• nealkoholová steatóza jater chemicky indukované   patologie   prevence a kontrola   MeSH
• novorozená zvířata MeSH
• polyfenoly farmakologie   MeSH
• potkani Wistar MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• tuková tkáň účinky léků   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• fotochemie MeSH
• melaniny fyziologie   chemie   ultrastruktura   MeSH
• ultrafialové záření MeSH
• velikost částic MeSH
• vztahy mezi strukturou a aktivitou MeSH

Effect of UV irradiation on free radicals in different types of melanins and melanin complexes with diamagnetic Cd(II) and paramagnetic Cu(II) was examined by the use of electron paramagnetic resonance (EPR) spectroscopy. The aim of this studies was to compare o-semiquinone free radicals formation in two model eumelanins synthesized from 3,4-dihydroxyphenylalanine (DOPA) and tyrosine in the presence of tyrosinase, and in synthetic pheomelanin, under exposition on ultraviolet, because of the important role of free radicals and melanins in human organism. UV may change free radical concentrations in melanin. Changes in EPR spectra of DOPA-melanin-Cd(II) and DOPA-melanin-Cu(II) complexes after UV irradiation were determined. Diamagnetic Cd(II) strongly increased free radical concentrations in DOPA-melanin. UV irradiation during 30 and 60 min slightly increased and decreased free radical concentrations in DOPA-melanin-Cd(II) complexes, respectively. Paramagnetic Cu(II) quenched free radical lines of DOPA-melanin, and only the Cu(II) signals were detected for both UV-irradiated and nonirradiated samples. Free radical concentration in both eumelanins increased after UV irradiation, but it decreased in irradiated pheomelanin. EPR spectra of free radicals in the studied samples were homogeneously broadened. Slow spin-lattice relaxation processes exist in all the examined melanins and DOPA-melanin-Cd(II) complexes. Fast spin-lattice relaxation processes characterized Cu(II) in DOPA-melanin-Cu(II) complexes.

• MeSH
• dihydroxyfenylalanin účinky záření   MeSH
• elektronová paramagnetická rezonance * metody   využití   MeSH
• epidermis imunologie   účinky záření   MeSH
• lidé MeSH
• melaniny * izolace a purifikace   účinky záření   MeSH
• melanocyty imunologie   účinky záření   MeSH
• statistika jako téma MeSH
• tyrosinasa biosyntéza   účinky záření   MeSH
• ultrafialové záření * diagnostické užití   škodlivé účinky   MeSH
• volné radikály izolace a purifikace   škodlivé účinky   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH