Histological and histochemical methods were used for the studies on 12 species of metacestodes of the genera Coronacanthus, Diorchis, Diplopylidium, Fimbriaria, Hymenolepis, Microsomacanthus, Rodentotaenia, and Triodontolepis. Two basic types of larvae, cysticercoid and cercoscolex, were found. The cyst structure is identical or similar in the cysticercoids using the same group of intermediate hosts (gammarus, planktonic crustaceans, coprophagous beetles, and terrestrial snails). The morphological structure of scolex and neck is the same as in adult cestodes. The length and number of hooks are definitive in morphologically differentiated metacestodes, while the scolex, rostellum and suckers grow still inside the definitive host. The excretory canals open separately at the end of neck in the cysticercoids and in a common opening at the body end of larva in the cercoscolex. Calcareous corpuscles are smaller and more numerous in the scolex and neck than in the cyst wall. Acid mucosubstances prevail in the tissues at the early stages of development. In morphologically differentiated metacestodes, the amount of neutral mucosubstances and glycogen increases. In ageing larvae, the amount of neutral mucosubstances and glycogen gradually decreases and later also decreases the amount of acid mucosubstances, proteins and lipids.

• MeSH
• aminokyseliny analýza   MeSH
• Cestoda analýza   anatomie a histologie   růst a vývoj   MeSH
• lipidy analýza   MeSH
• proteiny analýza   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH

Metacestodes of Mesocestoides sp. were recorded from Sturnus vulgaris (Passeriformes: Stumidae) in the Czech Republic in April 2002. They were found in a cutaneous cyst and in the thoracic region of the body cavity of the bird. This is the first record of metacestodes of Mesocestoides sp. in this host species in Europe as well as the first finding of the formation of a cutaneous cyst provoked by this parasite. Additional specimens from Apodemus agrarius (Mammalia: Rodentia) from Bulgaria and Lacerta agilis (Reptilia: Squamata) from the Czech Republic were compared with that from S. vulgaris. Sequence data from the V4 variable region (18S rDNA) were used to compare genetic variability among these and previously characterized isolates of Mesocestoides spp. A number of distinct clades were recognized, with metacestodes from L. agilis showing the highest degree of relative divergence.

• MeSH
• Cestoda anatomie a histologie   genetika   izolace a purifikace   MeSH
• DNA helmintů chemie   genetika   MeSH
• fylogeneze MeSH
• hrudník parazitologie   MeSH
• kůže parazitologie   MeSH
• molekulární sekvence - údaje MeSH
• polymerázová řetězová reakce MeSH
• RNA ribozomální 18S chemie   genetika   MeSH
• sekvence nukleotidů MeSH
• sekvenční seřazení MeSH
• zpěvní ptáci parazitologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Taeniosis-cysticercosis caused by Taenia crassiceps (Zeder, 1800) is a useful experimental model for biomedical research, in substitution of Taenia solium Linnaeus, 1758, studied during decades to develop effective vaccination, novel anti-helminthic drugs and diagnostic tools. Cysticercosis in mouse (Mus musculus Linnaeus) is achieved by the larval subculturing of the Wake Forest University (WFU) strain of T. crassiceps. Golden hamster, Mesocricetus auratus (Waterhouse), has been shown to be the most suitable host for adult forms of parasite in experimental taeniosis. Metacestodes of T. crassiceps WFU multiply by budding without restrictions once inoculated into the mouse, while the number of tapeworms developed from these larvae in hamsters remains highly variable. Three objectives have been proposed to improve the infection of T. crassiceps WFU in hamsters: (1) to re-evaluate the need of immune suppression; (2) to investigate the advantage of infecting hamsters with metacestodes with in vitro protruded scolices; and (3) to compare a number of tapeworms developed from metacestodes subcultured in hamsters against those proliferated in mice. Our results demonstrated that when the evagination of murine metacestodes was high, the number of T. crassiceps WFU adults obtained from hamsters was also high. Immunosuppressive treatment remains relevant for this experimental rodent model. The hamster-to-hamster cysticercosis-taeniosis by T. crassiceps overcame the mouse-to-hamster model in the yield of adult specimens. In vitro scolex evagination and metacestode asexual proliferation in hamsters place this rodent model by T. crassiceps WFU as the most affordable experimental models with taeniids.

• MeSH
• cysticercus fyziologie   růst a vývoj   MeSH
• cysticerkóza * MeSH
• imunosupresivní léčba * MeSH
• křeček rodu Mesocricetus MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• nemoci hlodavců * imunologie   patofyziologie   MeSH
• Taenia * fyziologie   růst a vývoj   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• Echinococcus izolace a purifikace   parazitologie   MeSH
• hlodavci parazitologie   MeSH
• Taenia izolace a purifikace   parazitologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Geografické názvy
• Francie MeSH

BACKGROUND: Peritoneal larval cestodiasis induced by Mesocestoides Vaillant, 1863 (Cyclophyllidea: Mesocestoididae) is a common cause of severe infections in domestic dogs and cats, reported also from other mammals and less frequently from birds. However, there is a limited knowledge on the taxonomy of causative agents of this disease. RESULTS: In the present study, we investigated a massive, likely lethal, infection of a song thrush Turdus philomelos (Passeriformes: Turdidae) by Mesocestoides sp. tetrathyridia. We performed combined morphological and phylogenetic analysis of the tetrathyridia and compared them with the materials obtained previously from other birds and mammals. The metrical data fitted within the wide range reported by previous authors but confirmed the limited value of morphological data for species identification of tetrathyridia of Mesocestoides spp. The molecular analyses suggested that the isolates represented an unidentified Mesocestoides sp. that was previously repeatedly isolated and sequenced in larval and adult forms from domestic dogs and cats in Europe, the Middle East and North Africa. In contrast to the present study, which found encysted tetrathyridia, four of the five previous studies that identified the same species described infections by acephalic metacestodes only. CONCLUSIONS: The tetrathyridia of the examined Mesocestoides sp. are described in the present study for the first time. However, the possible match with the species that were previously reported to infect birds remains uncertain. The phylogenetic analyses also suggested the rejection of two cases that were previously identified as Mesocestoides corti as they were likely caused by the same species as in the presently reported infection case. The newly provided DNA sequences should allow the assignment to species in the future, when adults of the genus Mesocestoides are more thoroughly sequenced.

• MeSH
• cysticerkóza přenos   veterinární   MeSH
• domácí zvířata parazitologie   MeSH
• fatální výsledek MeSH
• fylogeneze MeSH
• kočky MeSH
• Mesocestoides genetika   patogenita   MeSH
• nemoci koček parazitologie   přenos   MeSH
• nemoci psů parazitologie   přenos   MeSH
• psi MeSH
• savci parazitologie   MeSH
• sekvence nukleotidů MeSH
• zpěvní ptáci parazitologie   MeSH
• zvířata MeSH
• Check Tag
• kočky MeSH
• psi MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH

• MeSH
• Echinococcus anatomie a histologie   růst a vývoj   MeSH
• echinokokóza přenos   MeSH
• hlodavci parazitologie   MeSH
• stadia vývoje MeSH

• MeSH
• cestodózy veterinární   MeSH
• Cyprinidae parazitologie   MeSH
• nemoci ryb parazitologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH

An annotated list of larvae (metacestodes) of gryporhynchid tapeworms (Cestoda: Cyclophyllidea) from freshwater fishes in Africa is provided with numerous new host and geographical records. Newly collected materials from Burundi, Democratic Republic of the Congo, Kenya, Madagascar, Namibia, Senegal, South Africa, Sudan and Zimbabwe practically double the total number of species reported from African fish so far. We confirm the occurrence of 16 species (five unidentified to the species level and most likely representing new taxa) belonging to the genera Amirthalingamia Bray, 1974 (1 species), Cyclustera Fuhrmann, 1901 (2 species), Dendrouterina Fuhrmann, 1912 (1 species), Neogryporhynchus Baer & Bona, 1960 (1 species), Paradilepis Hsü, 1935 (4 species), Parvitaenia Burt, 1940 (5 species), and Valipora Linton, 1927 (2 species). Additionally, metacestodes of four unidentified species of Paradilepis and Parvitaenia are reported from fish for the first time. Rostellar hooks of all species are illustrated and their measurements are provided together with a host-parasite list. The molecular phylogenetic analysis based on partial LSU rDNA sequences offers the first insight into the internal phylogenetic relationships within the family. Together with the morphological observations, the present study provides a taxonomic baseline for future studies on this largely neglected, but widely distributed and relatively frequent, group of parasites of African fishes, including economically important cichlids like tilapias and cyprinids.

• MeSH
• Cestoda klasifikace   genetika   MeSH
• druhová specificita MeSH
• fylogeneze MeSH
• larva MeSH
• ribozomální DNA genetika   MeSH
• ryby parazitologie   MeSH
• sladká voda MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Alveolární hydatidóza v České republice: vytypování mezihostitelů životního cyklu parazitů, diagnostika a molekulárně biologická analýza larválních stádií.; The study of alveolar hydatidosis in the Czech Republic focused to the determination of intermediate hosts, protein and DNA analysis of metacestodes and serological examination of selected group of patients with a history of visit the endemic areas.

• MeSH
• Echinococcus klasifikace   patogenita   MeSH
• echinokokóza epidemiologie   MeSH
• interakce hostitele a parazita MeSH

• Konspekt
• Buněčná biologie. Cytologie
• NLK Obory
• parazitologie
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR

From August 2008 to July 2010, 1,471 fish belonging to the subfamily Goodeinae (representing 28 species) were collected from 47 localities across central Mexico and analyzed for helminth parasites. In addition, a database with all available published accounts of the helminth parasite fauna of goodeines was assembled. Based on both sources of information, a checklist containing all the records was compiled as a necessary first step to address future questions in the areas of ecology, evolutionary biology and biogeography of this host-parasite association. The checklist is presented in two tables, a parasite-host list and a host-parasite list. The checklist contains 51 nominal species, from 34 genera and 26 families of helminth parasites. It includes 8 species of adult digeneans, 9 metacercarie, 6 monogeneans, 3 adult cestodes, 9 metacestodes, 1 adult acanthocephalan, 1 cystacanth, 6 adult nematodes and 8 larval nematodes. Based on the amount of information contained in the checklist, we pose that goodeines, a subfamily of viviparous freshwater fishes endemic to central Mexico, might be regarded as the first group of wildlife vertebrate for which a complete inventory of their helminth parasite fauna has been completed.

• MeSH
• cizopasní červi klasifikace   MeSH
• Cyprinodontiformes parazitologie   MeSH
• helmintózy zvířat parazitologie   MeSH
• kontrolní seznam MeSH
• nemoci ryb parazitologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Mexiko MeSH