parameterization
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Long QT syndrome (LQTS) presents a group of inheritable channelopathies with prolonged ventricular repolarization, leading to syncope, ventricular tachycardia, and sudden death. Differentiating LQTS genotypes is crucial for targeted management and treatment, yet conventional genetic testing remains costly and time-consuming. This study aims to improve the distinction between LQTS genotypes, particularly LQT3, through a novel electrocardiogram (ECG)-based approach. Patients with LQT3 are at elevated risk due to arrhythmia triggers associated with rest and sleep. Employing a database of genotyped long QT syndrome E-HOL-03-0480-013 ECG signals, we introduced two innovative parameterization techniques-area under the ECG curve and wave transformation into the unit circle-to classify LQT3 against LQT1 and LQT2 genotypes. Our methodology utilized single-lead ECG data with a 200 Hz sampling frequency. The support vector machine (SVM) model demonstrated the ability to discriminate LQT3 with a recall of 90% and a precision of 81%, achieving an F1-score of 0.85. This parameterization offers a potential substitute for genetic testing and is practical for low frequencies. These single-lead ECG data could enhance smartwatches' functionality and similar cardiovascular monitoring applications. The results underscore the viability of ECG morphology-based genotype classification, promising a significant step towards streamlined diagnosis and improved patient care in LQTS.
- MeSH
- dospělí MeSH
- elektrokardiografie * metody MeSH
- genotyp MeSH
- lidé MeSH
- strojové učení * MeSH
- support vector machine MeSH
- syndrom dlouhého QT * genetika diagnóza patofyziologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
Měření hlasového pole (voice range profile, VRP) patří k základním akustickým metodám vyšetření hlasu, slouží pro hodnocení dynamického a výškového rozsahu hlasu. V studii je uvedený přehled poznatků o měření a parametrizaci hlasového pole a jejich souvislost s poruchami hlasu, hlasovým tréninkem, pohlavím a věkem. Ve studii jsou schematicky znázorněny typické pozorované jevy na hlasových polích hlasových profesionálů a studentů herectví z předchozí studie (10) autora. Studie je zároveň teoretickým úvodem k navazující studii o zjištěných projevech poruchy hlasu u mužů na hlasovém poli. Klíčová slova: hlasové pole, akustické metody, hlasový trénink
The measurement of the voice range profile (VRP) is a basic acoustic method used for the evaluation of dynamic and frequency ranges of the voice. The study provides an overview of knowledge about measurement and parameterization of VRP and their relationship with voice disorders, voice training, gender and age. In the study there are schematically shown typical phenomena observed on VRPs of voice professionals and beginning students of acting from the previous study (10) of the author. The study is also a theoretical introduction to the follow-up study investigating the manifestations of voice disorders on the VRP measurement in men. Keywords: voice range profile, acoustic method, voice training
- MeSH
- akustika řeči * MeSH
- dítě MeSH
- dospělí MeSH
- hlas * fyziologie MeSH
- kvalita hlasu MeSH
- lidé MeSH
- měření tvorby řeči MeSH
- mladiství MeSH
- poruchy hlasu diagnóza MeSH
- výzkum MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
The development of human sexual orientation remains a complex and multifaceted subject. It is often studied but its origins continue to elude us. In this preregistered study, our primary objective was to demonstrate the fraternal birth order effect (FBOE), which assumes a higher prevalence of older brothers in gay men than in their straight counterparts and which has also been recently recorded in lesbian women. Our second aim was to explore any potential impact of the FBOE on anal-erotic role orientation (AERO), both in gay and straight men. Our study sample included 693 gay men, 843 straight men, 265 lesbian women, and 331 straight women from Czechia and Slovakia. Employing a conventionally parameterized logistic regression model, we substantiated the FBOE among both gay men (OR = 1.35 for maternal older brothers) and lesbian women (OR = 1.71). These outcomes were confirmed by a more nuanced parameterization recently proposed by Blanchard (2022). Nonmaternal older brothers did not exhibit a significant influence on their younger brothers' sexual orientation. Contrary to some earlier reports, however, our data did not establish the FBOE as exclusive to gay men with the receptive AERO. Furthermore, our observations indicated a lower offspring count for mothers of gay men compared to mothers of straight men. Emphasizing the positive FBOE outcomes, we acknowledge the need for caution regarding the various options that can be used to estimate the familial influences on sexual orientation.
- MeSH
- dospělí MeSH
- heterosexualita statistika a číselné údaje psychologie MeSH
- homosexualita mužská * statistika a číselné údaje psychologie MeSH
- homosexualita ženská * statistika a číselné údaje MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- pořadí narození * MeSH
- sexuální a genderové menšiny statistika a číselné údaje psychologie MeSH
- sexuální chování statistika a číselné údaje MeSH
- sourozenci MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Slovenská republika MeSH
Photosystem II (PSII) is a multi-subunit pigment-protein complex and is one of several protein assemblies that function cooperatively in photosynthesis in plants and cyanobacteria. As more structural data on PSII become available, new questions arise concerning the nature of the charge separation in PSII reaction center (RC). The crystal structure of PSII RC from cyanobacteria Thermosynechococcus vulcanus was selected for the computational study of conformational changes in photosystem II associated to the charge separation process. The parameterization of cofactors and lipids for classical MD simulation with Amber force field was performed. The parametrized complex of PSII was embedded in the lipid membrane for MD simulation with Amber in Gromacs. The conformational behavior of protein and the cofactors directly involved in the charge separation were studied by MD simulations and QM/MM calculations. This study identified the most likely mechanism of the proton-coupled reduction of plastoquinone QB. After the charge separation and the first electron transfer to QB, the system undergoes conformational change allowing the first proton transfer to QB- mediated via Ser264. After the second electron transfer to QBH, the system again adopts conformation allowing the second proton transfer to QBH-. The reduced QBH2 would then leave the binding pocket.
The pancreas, liver and hypothalamus have a regulatory function in the glucose homeostasis. As the blood glucose level changes, these compartments react and the level changes again. Subsequently to this reaction, the interstitial glucose level changes with some delay. In this paper, I propose a hypothesis that the change of the blood glucose level includes information about the estimated rate with which the hypothalamus expects the blood glucose level to return to normal range, by means of regulatory mechanisms of glucose homeostasis. As the interstitial glucose level change reflects the blood glucose level change, I propose a method to estimate the blood-to-interstitial glucose level delay. It is an important factor for glucose level prediction. Once the delay was calculated, it was possible to relate the present blood glucose level and future interstitial glucose level with such coefficients, which do not seem to change over the time of the experiment to a significant extent. Perhaps, it is a parameterization of regulatory processes of glucose homeostasis, which could be possibly encoded within hypothalamus set-points. The delays were constant per subject and ranged from 7 min up to 34 min for hereditary hypertriglyceridemic rats of 230-480 g weight, in experiments with a variable glucose infusion rate.
- MeSH
- biologické modely MeSH
- extracelulární tekutina metabolismus MeSH
- homeostáza fyziologie MeSH
- hypertriglyceridemie krev MeSH
- hypothalamus metabolismus MeSH
- játra metabolismus MeSH
- krevní glukóza metabolismus fyziologie MeSH
- krysa rodu rattus MeSH
- monitorování fyziologických funkcí MeSH
- pankreas metabolismus MeSH
- reakční čas MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
We report a powerful method for capturing the time-resolved concentration profiles, liquid swelling and surface phenomena during the absorption of methane (CH4) in still liquid ethanol (C2D6O) and n-decane (n-C10D22) and at high spatial resolution (pixel size 21.07 μm) using neutron imaging. Absorption of supercritical methane was followed at two temperatures and two pressures of methane, namely 7.0, 37.8 °C and 80, 120 bar. Fick's second law, which was used in the liquid-fixed coordinates, enabled for an adequate parameterization of the observed concentration profiles and liquid levels using simple analytical expressions. For both studied liquids, anomalously slow diffusion was observed in the initial stage of the absorption experiment. This was ascribed to the slow formation of the surface excess on the interface, time constant ranged 130-275 s. The axial symmetry of the cell allowed for the tomographic reconstructions of the profiles of the menisci. Based on these profiles, contact angle and surface tension were evaluated using the Young-Laplace equation. Overall, neutron imaging made it possible to capture time- and space-resolved information from which the methane concentration, liquid level and meniscus shape under high-pressure conditions inside a cylindrical titanium vessel were quantitatively derived. Multiple characteristics of ethanol, a methane hydrate inhibitor, and n-decane, a model constituent of crude oil, were thus measured for the first time under industrially relevant conditions in a one-pot experiment.
... model terms 70\n\n5.4.4 Comparison of levels using contrasts 74\n\n5.4.5 Contrasts and the model parameterization ...
1. elektronické vydání 1 online zdroj (256 stran)
... model terms 70\n\n5.4.4 Comparison of levels using contrasts 74\n\n5.4.5 Contrasts and the model parameterization ...
First edition x, 245 stran : ilustrace ; 24 cm
- Konspekt
- Biologické vědy
- NLK Obory
- biologie
- statistika, zdravotnická statistika
- knihovnictví, informační věda a muzeologie
- NLK Publikační typ
- kolektivní monografie
BACKGROUND: Environmental DNA and metabarcoding allow the identification of a mixture of species and launch a new era in bio- and eco-assessment. Many steps are required to obtain taxonomically assigned matrices from raw data. For most of these, a plethora of tools are available; each tool's execution parameters need to be tailored to reflect each experiment's idiosyncrasy. Adding to this complexity, the computation capacity of high-performance computing systems is frequently required for such analyses. To address the difficulties, bioinformatic pipelines need to combine state-of-the art technologies and algorithms with an easy to get-set-use framework, allowing researchers to tune each study. Software containerization technologies ease the sharing and running of software packages across operating systems; thus, they strongly facilitate pipeline development and usage. Likewise programming languages specialized for big data pipelines incorporate features like roll-back checkpoints and on-demand partial pipeline execution. FINDINGS: PEMA is a containerized assembly of key metabarcoding analysis tools that requires low effort in setting up, running, and customizing to researchers' needs. Based on third-party tools, PEMA performs read pre-processing, (molecular) operational taxonomic unit clustering, amplicon sequence variant inference, and taxonomy assignment for 16S and 18S ribosomal RNA, as well as ITS and COI marker gene data. Owing to its simplified parameterization and checkpoint support, PEMA allows users to explore alternative algorithms for specific steps of the pipeline without the need of a complete re-execution. PEMA was evaluated against both mock communities and previously published datasets and achieved results of comparable quality. CONCLUSIONS: A high-performance computing-based approach was used to develop PEMA; however, it can be used in personal computers as well. PEMA's time-efficient performance and good results will allow it to be used for accurate environmental DNA metabarcoding analysis, thus enhancing the applicability of next-generation biodiversity assessment studies.
- MeSH
- Archaea MeSH
- Bacteria MeSH
- environmentální DNA chemie genetika MeSH
- houby MeSH
- metagenomika metody normy MeSH
- referenční standardy MeSH
- respirační komplex IV genetika MeSH
- RNA ribozomální 16S genetika MeSH
- RNA ribozomální 18S genetika MeSH
- rostliny MeSH
- senzitivita a specificita MeSH
- software MeSH
- taxonomické DNA čárové kódování metody normy MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
