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BACKGROUND: Radio frequency (RF) and chemical peels have been used for nonablative skin rejuvenation. Both of these cause collagen remodeling in the dermis and neo-collagen formation resulting in facial rejuvenation. There is limited literature on the evaluation of collagen remodeling by objective methods. OBJECTIVE: To compare the benefits of monopolar radiofrequency and glycolic acid peels in facial rejuvenation with regards to histopathology and Ultrabiomicroscopic sonography (UBM). METHODOLOGY: In this study, forty patients with mild to moderate photoaging received four treatments with 3 weeks interval of monopolar radiofrequency on one side of face and glycolic acid peels in increasing concentrations (NeostrataR) on the other side. Pre and post treatment, 2 mm biopsies were taken from both preauricular areas and Ultrasonography using a 35 MHz probe was done from outer canthus of eye and nasolabial folds from both sides of face. A blinded assessment was done to measure the increase in the grenz zone and dermal thickness. RESULTS: In 35/40 patients there was a significant increase in the grenz zone on histopathology and decrease in subepidermal low-echogenic band (SLEB) on UBM of the nasolabial folds on both sides of the face (p < 0.05). CONCLUSION: Radiofrequency and chemical peels showed equal efficacy in the treatment of facial rejuvenation.
- MeSH
- chemická exfoliace metody MeSH
- dospělí MeSH
- elektrostimulační terapie přístrojové vybavení MeSH
- glykoláty aplikace a dávkování MeSH
- keratolytika aplikace a dávkování MeSH
- lidé středního věku MeSH
- lidé MeSH
- obličej * patologie MeSH
- omlazení * MeSH
- rádiové vlny * MeSH
- spokojenost pacientů MeSH
- stárnutí kůže * účinky léků patologie účinky záření MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
Passiflora species, mangosteen, and cherimoya peels are a source of bioactive phenolic compounds. Nevertheless, a significant fraction of polyphenols, called non-extractable polyphenols (NEPs), are retained in the extraction residue after a conventional extraction. Thus, alkaline, acid, and enzymatic-assisted extractions to recover high contents of antioxidant NEPs from the extraction residue of fruit peels, were compared in this work. A high-performance thin-layer chromatography method with UV/Vis detection was developed in order to obtain the phenolic profile for the extracts. The most intense bands were further analyzed by direct analysis in real-time-high-resolution mass spectrometry to tentatively identified NEPs in fruit peel extracts. Total phenolic and proanthocyanidin contents and antioxidant capacity of the extracts were measured to carry out a multivariate statistical analysis. Alkaline hydrolysis was the most efficient treatment to recover NEPs from fruit peels as well as a promising treatment to obtain antioxidant extracts along with EAE. Cherimoya peel extracts were the richest in antioxidant NEPs. This work highlights that many NEPs remain on the extraction residue of fruit peels after conventional extraction and are not usually taken into account.
INTRODUCTION: Within oncology research, there is a high effort for new approaches to prevent and treat cancer as a life-threatening disease. Specific plant species that adapt to harsh conditions may possess unique properties that may be utilized in the management of cancer. HYPOTHESIS: Chokeberry fruit is rich in secondary metabolites with anti-cancer activities potentially useful in cancer prevention and treatment. AIMS OF THE STUDY AND METHODS: Based on mentioned hypothesis, the main goal of our study was to evaluate the antitumor effects of dietary administered Aronia melanocarpa L. fruit peels (in two concentrations of 0.3 and 3% [w/w]) in the therapeutic syngeneic 4T1 mouse adenocarcinoma model, the chemopreventive model of chemically induced mammary carcinogenesis in rats, a cell antioxidant assay, and robust in vitro analyses using MCF-7 and MDA-MB-231 cancer cells. RESULTS: The dominant metabolites in the A. melanocarpa fruit peel extract tested were phenolic derivatives classified as anthocyanins and procyanidins. In a therapeutic model, aronia significantly reduced the volume of 4T1 tumors at both higher and lower doses. In the same tumors, we noted a significant dose-dependent decrease in the mitotic activity index compared to the control. In the chemopreventive model, the expression of Bax was significantly increased by aronia at both doses. Additionally, aronia decreased Bcl-2 and VEGF levels, increasing the Bax/Bcl-2 ratio compared to the control group. The cytoplasmic expression of caspase-3 was significantly enhanced when aronia was administered at a higher dosage, in contrast to both the control group and the aronia group treated with a lower dosage. Furthermore, the higher dosage of aronia exhibited a significant reduction in the expression of the tumor stem cell marker CD133 compared to the control group. In addition, the examination of aronia`s epigenetic impact on tumor tissue through in vivo analyses revealed significant alterations in histone chemical modifications, specifically H3K4m3 and H3K9m3, miRNAs expression (miR155, miR210, and miR34a) and methylation status of tumor suppressor genes (PTEN and TIMP3). In vitro studies utilizing a methanolic extract of A.melanocarpa demonstrated significant anti-cancer properties in the MCF-7 and MDA-MB-231 cell lines. Various analyses, including Resazurin, cell cycle, annexin V/PI, caspase-3/7, Bcl-2, PARP, and mitochondrial membrane potential, were conducted in this regard. Additionally, the aronia extract enhanced the responsiveness to epirubicin in both cancer cell lines. CONCLUSION: This study is the first to analyze the antitumor effect of A. melanocarpa in selected models of experimental breast carcinoma in vivo and in vitro. The utilization of the antitumor effects of aronia in clinical practice is still minimal and requires precise and long-term clinical evaluations. Individualized cancer-type profiling and patient stratification are crucial for effectively implementing plant nutraceuticals within targeted anti-cancer strategies in clinical oncology.
- Publikační typ
- časopisecké články MeSH
This study sought to characterize the soluble free and bound phenolic compounds from shaddock (Citrus maxima) peels and investigate their effect on 3-hydroxy-methyl-3-glutaryl coenzyme A reductase (HMG-CoA reductase) and glutathione-linked enzymes in colon (Caco-2) cells. The radicals scavenging ability and the protective ability of the phenolic extracts against pro-oxidant induced oxidative damage in Caco-2 cells and rat colon homogenates were also investigated. The free phenolics were extracted with 80% acetone (v/v), while bound phenolics were extracted from the alkaline (NaOH) and acid (HCl) hydrolyzed residue with ethyl acetate. HPLC fingerprinting revealed the presence of gallic acid, catechin, chlorogenic acid, caffeic acid, ellagic acid, epicatechin, rutin, quercitrin and quercetin in the extracts. The results revealed that the extracts inhibited HMG-CoA reductase activity and increased the activities of glutathione reductase and glutathione peroxidase in Caco-2 cells. The extracts inhibited peroxyl radical induced oxidation of membrane lipids in Caco-2 cells and malondialdehyde production in rat colon homogenates. Furthermore, the phenolic extracts scavenged radicals [2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH)] and chelated Fe2+ in a concentration-dependent manner. This study showed that shaddock peels could serve as a dietary means or nutraceutical source for protecting the colon from degeneration.
- MeSH
- Citrus chemie MeSH
- fenoly metabolismus MeSH
- glutathionreduktasa metabolismus účinky léků MeSH
- hydroxymethylglutaryl-CoA-reduktasy metabolismus účinky léků MeSH
- kolon * enzymologie metabolismus účinky léků MeSH
- oxidační stres účinky léků MeSH
- potkani Wistar MeSH
- rostlinné extrakty MeSH
- scavengery volných radikálů metabolismus MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
Chemický peeling se používá nejčastěji jako doplněk léčby akné, dále také k rejuvenaci a k zesvětlení pigmentací. Ne všechny pigmentové projevy na kůži jsou však vhodné k zesvětlování chemickým peelingem. Nejprve je nutná přesná diagnostika pigmentového ložiska, které je plánováno zesvětlit nebo odstranit a pak rozhodnout o vhodné metodě ošetření. Další možnou a modernější metodou odstraňování některých pigmentací je ošetření laserem. Ačkoli toto ošetření je obvykle účinnější, není to metoda, která je vždy dobře dostupná, a tak chemický peeling má v praxi stále své místo. Některé nověji vyráběné chemické peelingy je možné aplikovat bez předchozí přípravy a následné reakce kůže nejsou tak výrazné, takže nedochází k většímu společenskému omezení pacienta v průběhu léčby, a komfort ošetření je tedy lepší.
Chemical peeling is most often used as an adjunct to acne treatment as well as for rejuvenation and pigmentation lightening. However, not all skin pigments are suitable for lightening with chemical peeling. First, it is necessary to precisely diagnose the pigmented spot intended to be lightened or removed, and next to determine the appropriate method of treatment. Another possible and more modern method of removing some pigmentations is laser treatment. Although this treatment is usually more effective, it is a method that is not always readily available; therefore, chemical peeling still has its place in the practice. Some newly produced chemical peels can be applied without previous preparation and the subsequent skin reactions are not so prominent; as a result, there is no major social limitation on the patient during the course of therapy, thus resulting in better treatment comfort.
Principem chemických metod je plošný a ohraničený účinek látek označovaných jako kaustika. Používáním těchto látek, převážně alfahydroxykyselin – ovocných kyselin, fenolu, kyseliny trichloroctové, vede k navození regeneračních procesů v kůži. Mechanické obrušování pleti pomocí speciálních krémů a past s abrazivními přísadami se nazývá mechanický peeling.
The principle of chemical methods is surface circumscribed effect of substances described as caustics. The use of these substances, mainly alpha-hydroxy-acid-fruit acids, phenol, and trichloracetic acid, leads to stimulation of regeneration processes in skin. Mechanic abrasion of skin with use of special creams and pastes with abrasive substances is called mechanical peeling.
- MeSH
- chemická exfoliace * MeSH
- dermatologie MeSH
- erytém terapie MeSH
- hydroxykyseliny MeSH
- kyselina trichloroctová MeSH
- lidé MeSH
- obličej MeSH
- péče o kůži * MeSH
- péče o sebe MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Program H plus s. r. o., Praha Chemický peeling je aplikace chemické látky na kůži za účelem kontrolovaného poškození epidermis a části dermis, kdy následným sekundárním hojením dojde k regeneraci z adnexálních struktur s výsledným zlepšením barvy a textury kůže. Hloubka průniku závisí na typu a koncentraci chemické látky, přípravě před zákrokem, lokalizaci, fototypu a míře aktinického poškození kůže. Kromě kosmetických indikací jako odstranění hyperpigmentací, zlepšení akné, jizev a jemných vrásek má CHP velký význam v prevenci a léčbě prekanceróz a nemelanomových kožních nádorů.
Chemical peel is application of chemical agent to the skin that results in injury of the epidermis and dermis followed by second-intention wound healing, regeneration from adnexal structures and leads to improvement of color and texture of the skin. The depth of penetration depends on the type and concentration of chemical agent, preoperative preparation, localisation, skin type and degree of photo-damage. Except cosmetic indications such as treatment of hyperpigmentation, acne, scars and wrinkles, CHP is very important in prevention and treatment of precancerosis and non-melanoma skin cancer.
- Klíčová slova
- komplikace, indikace, středně hluboký a hluboký chemický peeling, povrchový,
- MeSH
- chemická exfoliace metody využití MeSH
- dospělí MeSH
- farmakopea jako téma MeSH
- kontraindikace MeSH
- kosmetické techniky trendy využití MeSH
- kožní nemoci MeSH
- lidé MeSH
- prekancerózy terapie MeSH
- stárnutí kůže účinky léků MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
