Aromatic cytokinins (ortho-topolin riboside, 6-benzylaminopurine riboside and 6-(2-hydroxy-3-methoxybenzyla mino)purine riboside) were tested for their possible interaction with human liver microsomal cytochromes P450 by absorption difference spectroscopy. All three compounds were shown to bind to the CYP enzymes producing a high to low spin shift of the heme iron yielding a Soret absorption band shift to approximately 425 nm. As this type of spectral change means that the substance is able to bind directly to the heme iron, the results obtained open the possibility of an interaction of these compounds with metabolism of other drugs or, in general, with other substrates of cytochromes P450.
Microtubule disruptors, widely known as antimitotics, have broad applications in human medicine, especially as anti-neoplastic agents. They are subject to biotransformation within human body frequently involving cytochromes P450. Therefore antimitotics are potential culprits of drug-drug interactions on the level of activity as well as expression of cytochromes P450. This review discusses the effects of four well-known natural antimitotics: colchicine, taxol (paclitaxel), vincristine, and vinblastine, and a synthetic microtubule disruptor nocodazole on transcriptional activity of glucocorticoid and aryl hydrocarbon receptors. It appears that microtubules disarray restricts the signaling by these two nuclear receptors regardless of cell cycle phase. Consequently, intact microtubules play an important role in the regulation of expression of cytochromes P450, which are under direct or indirect control of the two nuclear receptors.
- MeSH
- antimitotika farmakologie MeSH
- biotransformace MeSH
- kolchicin farmakologie MeSH
- lékové interakce MeSH
- lidé MeSH
- mikrotubuly účinky léků MeSH
- nokodazol farmakologie MeSH
- paclitaxel farmakologie MeSH
- receptory aromatických uhlovodíků metabolismus MeSH
- receptory glukokortikoidů metabolismus MeSH
- signální transdukce MeSH
- systém (enzymů) cytochromů P-450 metabolismus MeSH
- vinblastin farmakologie MeSH
- vinkristin farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
Enzyme activities of the CYP enzymes (CYP3A4, CYP2C9 and CYP2A6) were determined using classical substrates (testosterone, diclofenac and coumarin, respectively) as well as with luminogenic or fluorogenic substrates in micromethod arrangement. The luciferin-based luminogenic substrates for CYP3A4 and CYP2C9 as well as coumarin in micromethod for assay of CYP2A6 activity gave results well comparable with the classical methods with determination of reaction products by the HPLC.
- MeSH
- aromatické hydroxylasy analýza MeSH
- cytochrom P-450 CYP3A MeSH
- diklofenak chemie MeSH
- kumariny chemie MeSH
- lidé MeSH
- oxygenasy se smíšenou funkcí analýza MeSH
- systém (enzymů) cytochromů P-450 analýza MeSH
- testosteron chemie MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- MeSH
- antioxidancia analýza farmakologie MeSH
- Asteraceae MeSH
- fenoly farmakologie MeSH
- finanční podpora výzkumu jako téma MeSH
- krysa rodu rattus MeSH
- kultivované buňky MeSH
- rostlinné extrakty farmakologie MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- MeSH
- antiinfekční látky farmakologie MeSH
- antioxidancia aplikace a dávkování farmakologie terapeutické užití MeSH
- antitumorózní látky fytogenní aplikace a dávkování terapeutické užití MeSH
- finanční podpora výzkumu jako téma MeSH
- fytoterapie MeSH
- myši MeSH
- rostlinné extrakty farmakologie MeSH
- scavengery volných radikálů MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- MeSH
- alkaloidy toxicita MeSH
- fenantridiny toxicita MeSH
- játra účinky léků MeSH
- kultivované buňky MeSH
- Publikační typ
- techniky in vitro MeSH
The cyclic voltammetry (CV) was used for the measurement of the plasma total antioxidant capacity from two types of patients. The first one consisted of 29 volunteers (men aged 18-21 years) who were administered placebo or silymarin at a dose of 858 mg/day. After two months of silymarine administration, CV revealed a statistically significant increase in total antioxidant capacity compared to placebo. No statistically significant changes in TBARS, SH-groups, creatininin, urea, and uric acid concentrations were found. The second group under study comprised 49 patients with chronic renal disease during dialysis therapy. After dialysis, CV revealed a decrease of total antioxidant capacity in the plasma, which was equivalent to a decrease in creatinine, urea and uric acid. CV was performed using a system consisting of a working glassy carbon electrode, an auxiliary platinum electrode, and a reference saturated calomel electrode; a linear change of voltage of 200 mV/s was applied. CV is a simple and relatively reliable method for assessment of body antioxidant status. It is also time and cost effective.
- MeSH
- antioxidancia metabolismus MeSH
- chronická nemoc MeSH
- dialýza ledvin MeSH
- dospělí MeSH
- elektrochemie metody MeSH
- krev metabolismus MeSH
- lidé MeSH
- nemoci ledvin krev terapie MeSH
- referenční hodnoty MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Osteoprotegerin, RANK (Receptor Activator of Nuclear factor kappa B) and RANKL (Receptor Activator of Nuclear faktor kappa B ligand) became the aim of intensive research. RANK is considered as a hematopoietic surface receptor controlling osteoclastogenesis and calcium metabolism. RANKL may promote osteoresorption by induction of cathepsin K gene expression. The present paper summarizes the most significant data in osteoprotegerin, RANK and RANKL problems obtained.
- MeSH
- glykoproteiny fyziologie MeSH
- lidé MeSH
- ligand RANK MeSH
- membránové glykoproteiny fyziologie MeSH
- osteoklasty fyziologie MeSH
- osteoprotegerin MeSH
- protein RANK MeSH
- receptory cytoplazmatické a nukleární fyziologie MeSH
- receptory TNF MeSH
- transportní proteiny fyziologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
