BACKGROUND: The diagnosis of Creutzfeldt-Jakob disease (CJD) is based on typical clinical features and can be supported by detection of 14-3-3 protein in the CSF. The present study suggests the importance of investigating this ratio of total tau protein to phosphorylated tau protein in differentiating CJD from other dementias. Thirty-one patients with Alzheimer's disease (AD) or frontotemporal dementia and four with definitive diagnoses of CJD were included in the study. METHODS AND MATERIAL: Results from baseline investigations were compared with those from an age-matched cognitively controlled group with Bell's palsy. Tau protein, phosphorylated tau protein, and beta amyloid were analyzed using a commercially available enzyme-linked immunosorbent assay; 14-3-3 protein was assessed by Western blotting. RESULTS AND CONCLUSION: A distinctly high proportion of total tau protein to phosphorylated tau protein in CSF was found in all patients diagnosed with CJD, even in those with negative 14-3-3 protein blot results. In contrast, marker analysis in patients with Alzheimer's dementia revealed the highest CSF ratio of beta amyloid to phosphorylated tau protein levels. These proteins are important diagnostic biomarkers for CJD, especially in patients with negative 14-3-3 protein findings.
- MeSH
- Alzheimerova nemoc krev MeSH
- Creutzfeldtova-Jakobova nemoc krev MeSH
- demence diagnóza krev MeSH
- diferenciální diagnóza MeSH
- fosforylace MeSH
- lidé středního věku MeSH
- lidé MeSH
- proteiny 14-3-3 MeSH
- reprodukovatelnost výsledků MeSH
- senioři MeSH
- senzitivita a specificita MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- hodnotící studie MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Přeruš. str. : il., tab. ; 31 cm
We want to measure autoantibodies directed to protein beta-tubulin class III, generated by specific immunity as a marker of the axonal loss together with the progression of Multiple Sclerosis in cooperation with MS center 2nd medical faculty UK.
Ve spolupráci s MS centrem , které bude dodávat séra svých pacientů, chceme měřit autoprotilátky proti proteinu beta-tubulinu class III, generované specifickou imunitou, jako ukazatele postupu axonální ztráty, a tím také ukazatele stavu a vývoje choroby.
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- neurologie
- neurovědy
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
- Publikační typ
- abstrakt z konference MeSH
Brain trauma typically leads to neuronal damage and loss. Assuming a transient autoimmune response to debris of the damaged neurones, we have monitored serum titres of IgG and IgM antibodies to beta-tubulin class III (betaTcIII), which is almost exclusively found in neuronal cytoskeletons. In 15 out of 18 patients, the peak of the IgG or IgM antibody titre appeared in the serum within 3 weeks of a brain trauma.
