AIMS: Our aim was to describe the changes in therapy and diabetes control in Ukrainian war refugee children with diabetes (CwD) during the first year of their stay in Czechia. METHODS: A total of 124 CwD (62 male, 62 female) were enrolled into this observational study. Anthropometric, laboratory and diabetes management data were acquired at baseline and at 3 months intervals for 12 months. All CwD were offered a CGM device during their first visit. Generalized Estimating Equation models were fitted in order to estimate the dynamics of studied characteristics. RESULTS: Median baseline HbA1c was 58 mmol/mol (IQR [48; 73]mmol/mol) (7.5 %, IQR[6.5;8.8]%). The HbA1c decreased significantly throughout the course of the study at a pace of - 2.2 mmol/mol (-0.2 %pt.) per visit (P = 0.01, CI[-3.2;-1.1]). The pace of the decrease in the average HbA1c was significantly higher in the group of CwD who received CGM in Czechia than in those who already had it from Ukraine by 2.9 mmol/mol (0.27 %pt.) per visit (P < 0.001, CI [-4.4; -1.3]). CONCLUSIONS: The steepest decrease in HbA1c was observed in CwD with newly initiated CGM underlining its vital role in improving the glucose control of CwD regardless of their background.
- MeSH
- diabetes mellitus 1. typu * farmakoterapie MeSH
- dítě MeSH
- glykovaný hemoglobin MeSH
- kontinuální monitorování glukózy MeSH
- krevní glukóza MeSH
- lidé MeSH
- selfmonitoring glykemie MeSH
- uprchlíci * MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- pozorovací studie MeSH
Surface plasmon resonance (SPR) biosensors capable of in real time detection of Cronobacter at concentrations down to 10⁶ cells mL⁻¹ in samples of consumer fresh-whole fat milk, powder whole-fat milk preparation, and powder infant formulation were developed for the first time. Antibodies against Cronobacter were covalently attached onto polymer brushes of poly(2-hydroxyethyl methacrylate) (poly(HEMA)) grafted from the SPR chip surface. The lowest detection limit, 10⁴ cells mL⁻¹, was achieved in phosphate buffered saline (pH 7.4) with sensors prepared by covalent immobilization of the same antibodies onto a self assembled monolayer (SAM) of hexa(ethylene glycol) undecanethiol (EG₆). However, when the EG₆ based sensors were challenged with milk samples the non-specific response due to the deposition of non-targeted compounds from the milk samples was much higher than the specific response to Cronobacter hampering the detection in milk. Similar interfering fouling was observed on antifouling polymer brushes of hydroxy-capped oligoethylene glycol methacrylate and even a 10 times higher fouling was observed on the widely used SAM of mixed hydroxy- and carboxy-terminated alkanethiols. Only poly(HEMA) brushes totally suppressed the fouling from milk samples. The robust well-controlled surface initiated atom transfer radical polymerization of HEMA allowed the preparation of highly dense brushes with a minimal thickness so that the capture of antigens by the antibodies immobilized on the brush layer could take place close to the gold SPR surface to provide a stronger optical response while the fouling was still suppressed. A minimum thickness of 19 nm of poly(HEMA) brush layer was necessary to suppress completely non-specific sensor response to fouling from milk.
- MeSH
- Enterobacteriaceae izolace a purifikace patogenita MeSH
- imobilizační protilátky MeSH
- kojenec MeSH
- lidé MeSH
- limita detekce MeSH
- mléko mikrobiologie MeSH
- náhražky mateřského mléka MeSH
- polyhydroxyethylmethakrylát MeSH
- potravinářská mikrobiologie metody statistika a číselné údaje MeSH
- povrchová plasmonová rezonance metody statistika a číselné údaje MeSH
- protilátky bakteriální MeSH
- zlato MeSH
- zvířata MeSH
- Check Tag
- kojenec MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
Nuclease from tomato (TBN1) was produced by in planta biotechnology purified and tested for its anticarcinogenic properties. The nuclease was cytostatic after its intratumoral administration to nude mice bearing human melanoma or prostate carcinoma or after tumor targeting by TBN1 administration intravenously as conjugate with polyethylene glycol (PEG). Inhibitory effects of TBN1 on tumor growth were comparable to effects of bovine seminal RNase (BS-RNase), but the inhibition was reached at about ten times lower protein concentration. Simultaneously, TBN1 exhibited a lower degree of embryotoxicity compared to BS-RNAse and other nucleases. TBN1 showed significant stability in vivo, because it was readily detected after its administration intratumorally or intravenously by the fluorescence methods. Intravenous administration of TBN1-PEG caused significant inhibition of tumor proliferation without obvious degenerative changes, while direct administration of TBN1 into melanoma tumors led to rapid tumor tissue degeneration. The fact can be essential for the mode of TBN1 biological action that mature nuclease is a small (36 kDa) thermostable glycoprotein that has ability to destroy human 28S, 18S, 7S and 5.8S RNA, circular RNAs, double-stranded RNA in vitro and shows DNase and 3'nucleotidase activities.
- MeSH
- antitumorózní látky farmakologie MeSH
- apoptóza účinky léků MeSH
- dvouvláknová RNA genetika metabolismus MeSH
- embryo savčí enzymologie patologie MeSH
- endodeoxyribonukleasy farmakologie MeSH
- fluorescenční protilátková technika MeSH
- glykosylace MeSH
- lidé MeSH
- melanom experimentální patologie MeSH
- míra přežití MeSH
- myši nahé MeSH
- myši MeSH
- nádorové buňky kultivované MeSH
- nádory prostaty patologie MeSH
- rekombinantní proteiny terapeutické užití MeSH
- skot MeSH
- Solanum lycopersicum enzymologie MeSH
- testikulární nádory patologie MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- autoprotilátky krev MeSH
- diabetes mellitus 1. typu genetika prevence a kontrola MeSH
- dítě MeSH
- dvojitá slepá metoda MeSH
- finanční podpora výzkumu jako téma MeSH
- fyziologie výživy kojenců MeSH
- kojenec MeSH
- lidé MeSH
- mléko MeSH
- Check Tag
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- diabetes mellitus MeSH
- dietní sacharidy MeSH
- mladiství MeSH
- průzkumy a dotazníky MeSH
- Check Tag
- mladiství MeSH
- Publikační typ
- kongresy MeSH
- MeSH
- antispermatogenní látky farmakologie chemie MeSH
- antitumorózní látky farmakologie chemie MeSH
- endoribonukleasy farmakologie chemie MeSH
- injekce intraperitoneální MeSH
- lidé MeSH
- melanom experimentální farmakoterapie MeSH
- myši MeSH
- pankreatická ribonukleasa farmakologie chemie MeSH
- polyethylenglykoly chemie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
