The 16S-23S ribosomal internal transcribed spacer (ITS1) is often used as a subspecies or strain-specific molecular marker for various kinds of bacteria. However, the presence of different copies of ITS1 within a single genome has been reported. Such mosaicism may influence correct typing of many bacteria and therefore knowledge about exact configuration of this region in a particular genome is essential. In order to screen the variability of ITS1 among and within Pseudomonas syringae genomes, an oligonucleotide microarray targeting different configurations of ITS1 was developed. The microarray revealed seven distinct variants in 13 pathovars tested and detected mosaicism within the genomes of P. syringae pv. coronafaciens, pisi, syringae and tabaci. In addition, the findings presented here challenge the using of rRNA analysis for pathovar and strain determination.
- MeSH
- DNA, Bacterial genetics MeSH
- Phylogeny MeSH
- Genetic Variation MeSH
- DNA, Ribosomal Spacer genetics MeSH
- Molecular Sequence Data MeSH
- Plant Diseases microbiology MeSH
- Pseudomonas syringae classification genetics isolation & purification MeSH
- RNA, Ribosomal, 16S genetics MeSH
- RNA, Ribosomal, 23S genetics MeSH
- Plants microbiology MeSH
- Base Sequence MeSH
- Oligonucleotide Array Sequence Analysis methods MeSH
- Sequence Alignment MeSH
- Bacterial Typing Techniques MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- MeSH
- DNA Fingerprinting methods utilization MeSH
- Research Support as Topic MeSH
- Gram-Positive Bacteria immunology classification MeSH
- Polymerase Chain Reaction methods utilization MeSH
- Publication type
- Comparative Study MeSH
- Geographicals
- Czech Republic MeSH
- MeSH
- Erwinia immunology MeSH
- Serologic Tests MeSH
- Publication type
- Comparative Study MeSH
