BACKGROUND: Grasses belong to major sources of inhaled allergens. The knowledge of particular molecules responsible for hypersensitivity is of crucial importance for better understanding of individual differences among single allergic subjects and allergic populations living in various world-areas. METHODS: Specific-IgE-antibodies against Phl p 1, Phl p 5, Phl p 7, Phl p 12 were detected in a group of 130 Phleum-allergic-subjects (82 children, 48 adults). RESULTS: Phl p 1 antibodies were detected in most pediatric and adult patients, however, the children were associated with higher RAST classes more often. Anti-Phl p 5-antibodies were found more frequently in adults. An increase was observed in the number of pediatric patients reacting to Phl p 7 and Phl p 12. There were no differences in concentrations of specific-IgE against Phl p 5, Phl p 7 and Phl p 12 depending on age. Almost 10% of allergic children produced antibodies directed exclusively against minor allergens or did not produce specific-IgE-antibodies against tested molecules. Part of the patients reacted to profilin and calcium-binding protein originating from only one source (Phl p 12/Bet v 2 and Phl p 7/Bet v 4). CONCLUSIONS: Antibodies against Phl p 1 and Phl p 5 can be used as a marker of allergy to grasses in adult patients. Children reacted exclusively to minor allergens more frequently than adults. Prolonged allergen exposure is evidently necessary to induce sensitization to Phl p 5. A high level of homology between profilins and calcium-binding proteins enables only one allergen to be used for diagnostic purposes but a possibility of a reaction to species-bound epitopes should be taken into account.
- MeSH
- alergeny imunologie MeSH
- antigeny rostlinné imunologie MeSH
- biologické markery krev MeSH
- dítě MeSH
- dospělí MeSH
- epitopy MeSH
- imunoglobulin E krev MeSH
- kojenec MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- Phleum imunologie MeSH
- předškolní dítě MeSH
- profiliny imunologie MeSH
- proteiny vázající vápník imunologie MeSH
- pyl škodlivé účinky imunologie MeSH
- rostlinné proteiny imunologie MeSH
- senioři MeSH
- sezónní alergická rýma krev diagnóza imunologie MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- kojenec MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: Birch pollen belongs to the major allergen triggers in the spring season in Europe. Our rapidly expanding knowledge of the allergenic molecules enables us to better recognize the individual differences between the reactivity of specific IgE antibodies of individual patients and allergic populations living in various regions of the world. METHOD: In a group of birch pollen-allergic patients living in the Czech Republic (107 children, 71 adults) we detected the presence of Bet v1, Bet v2 and Bet v4 specific IgE antibodies. RESULTS: Bet v1 specific IgE antibodies were identified in most patients without any significant differences between children and adults. Bet v2 positivity was found more frequently in the group of children than in adults (p = 0.02). In most adult patients Bet v1 monospecificity was more expressed as compared to the pediatric group. More allergic subjects reacted against minor birch allergens in the pediatric group (p = 0.02). Specific IgE antibodies against Bet v1 were not detected in 10% of the tested patients. In this group, 5% of birch pollen-allergic patients were found to not have specific IgE antibodies against any of the tested recombinant allergens. CONCLUSION: The investigation of specific IgE antibodies against Bet v1, Bet v2 and Bet v4 demonstrated that the specificity of allergen-induced IgE antibodies in birch pollen-allergic individuals is dependent not only on the region in which a patient lives but also on age. Especially in children, there is an increase in the number of allergic subjects who do not react exclusively against the major allergen. The question is whether some allergen-specific IgE antibodies will disappear depending on age or on the contrary whether their synthesis will be increased.
- MeSH
- alergie imunologie MeSH
- antigeny rostlinné imunologie MeSH
- bříza imunologie MeSH
- dítě MeSH
- dospělí MeSH
- imunoglobulin E krev imunologie MeSH
- kojenec MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- předškolní dítě MeSH
- proteiny vázající vápník imunologie MeSH
- pyl imunologie MeSH
- rostlinné proteiny imunologie MeSH
- senioři MeSH
- věkové faktory MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- kojenec MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Přeruš. str. : il. ; 32 cm
Cílem úkolu je zdokonalení imunodiagnostiky larvální toxokarózy propracováním metody imunoblotu a sledování korelace s ELISA reakcí. Využití detekce vybraných specifických imunoglobulínů pro posouzení průběhu nákazy a úspěšnosti užité terapie.
- MeSH
- parazitární nemoci terapie diagnóza patologie MeSH
- sérologické testy metody MeSH
- Toxocara canis patogenita MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- parazitologie
- infekční lékařství
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
- MeSH
- cyproteron MeSH
- hydrokortison MeSH
- imunosupresivní léčba MeSH
- lymfocyty MeSH
- myši MeSH
- Check Tag
- myši MeSH
Neonatal skin grafts across a weak histocompatibility barrier (MSA-incompatible) were used only for sensitization of the recipients whose responsiveness was then tested in two different assays. On the basis of 1. functional inactivation of PEC in a PEC transfer system and 2. inhibition of macrophage migration. The difference between neonatal and adult skin grafts turned out to be quantitative rather than qualitative, i.e., both induced sensitization whose demonstration with the neonatal grafts required more sensitive techniques and/or more favourable timing because it was weaker. The possible nature of the difference, which was occasionally interpreted as being due to a tolerogenic rather than immunogenic activity of neonatal grafts, is discussed.
- MeSH
- histokompatibilní antigeny imunologie MeSH
- homologní transplantace MeSH
- imunologická tolerance * MeSH
- isoantigeny imunologie MeSH
- kůže imunologie MeSH
- lymfocyty imunologie MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- novorozená zvířata imunologie MeSH
- rejekce štěpu MeSH
- transplantace kůže * MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
