Adipocytokines secreted by adipose tissue are suggested to play a role in the development of obesity-related complications. Regular aerobic exercise has been shown to reduce the risk of metabolic complications in obese subjects. The aim of this study was to investigate the effect of aerobic training on gene expression in subcutaneous abdominal adipose tissue (SCAAT) and on plasma levels of several adipocytokines in obese women. Twenty-five obese sedentary premenopausal women (body mass index, 32.18 +/- 3.17 kg/m(2)) underwent a 12-week aerobic exercise program, with a frequency of 5 d/wk and intensity corresponding to 50% of individual maximal oxygen consumption (V(.-)(O(2)max)) consisting of 2 sessions per week of supervised aerobic exercise and 3 sessions per week of home-based exercise on a bicycle ergometer. Before and after the aerobic training, (V(.-)(O(2)max)) and body composition were measured and plasma and SCAAT biopsy samples (in a subgroup of 8 subjects) were obtained for determination of plasma and messenger RNA levels of adipocytokines (leptin, adiponectin, interleukin 6, tumor necrosis factor alpha). The aerobic training resulted in an increase of subjects' V o(2)max by 12.8% (24.6 +/- 3.9 vs 27.7 +/- 4.8 mL x min(-1) x kg(-1), P < .05). Body weight and fat mass were reduced by 5.9% (88.5 +/- 8.2 vs 83.3 +/- 7.7 kg, P < .001) and 6.4% (38.8 +/- 4.2% vs 36.3 +/- 4.6%, P < .001), respectively, and the revised QUantitative Insulin sensitivity ChecK Index (QUICKI) increased (0.43 +/- 0.06 vs 0.48 +/- 0.06, P < .05) during the aerobic training. No aerobic training-induced changes in messenger RNA levels of the investigated genes in SCAAT were observed. A decrease of plasma leptin (24.3 +/- 8.7 vs 18.1 +/- 8.3 ng/mL, P < .05) was detected, whereas plasma levels of other cytokines remained unchanged. In moderately obese females, 3 months' aerobic training did not promote changes in the adipose tissue gene expression or plasma levels of the adipocytokines (except for leptin) involved in a regulation of lipid and carbohydrate metabolism.
- MeSH
- adiponektin biosyntéza genetika MeSH
- břišní tuk MeSH
- cvičení fyziologie MeSH
- dieta MeSH
- dospělí MeSH
- financování organizované MeSH
- interleukin-6 biosyntéza genetika MeSH
- kinetika MeSH
- krevní glukóza metabolismus MeSH
- lidé MeSH
- messenger RNA biosyntéza genetika MeSH
- obezita genetika metabolismus MeSH
- prospektivní studie MeSH
- složení těla MeSH
- spotřeba kyslíku fyziologie MeSH
- tělesná výkonnost MeSH
- TNF-alfa biosyntéza genetika MeSH
- triglyceridy krev MeSH
- tuková tkáň metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Přeruš. str. : il., tab., grafy ; 32 cm
The aim of the project is to form a program of strenght training and evaluate its effect on characteristics of metab. syndrom and on adipose tissue metab. during a 12 week´s intervention in nondiabetic obese subjects and in diabetic pat. with overweight.The attention will be paid to insulin resistence evaluated using hyperinsulinemic clamp, to adipose tissue metabolism evaluated in vivo using the micridialysis methiod and to expression of genes (mRNA) involved in the regulation of adipose tissue metab.
Předmětem projektu je sestavení programu pohybové aktivity dynamického silového typu a sledování vlivu 12 týdenní intervence tímto programem na ukazatele charakterizující metabolický sy a metabol tukové tkáně u obesních jedinců a u diabetiků s nadváhou.Sledována bude insulinová resistence metodou hyperinsulinového klempu, metabolismus tukové tkáně bude sledován in vivo metodou mikrodialysy tukové tkáně, in vitro na isolovaných adipocytech. Dále in vitro bude metodou polymerasové řetězové reakce (PCR) stanovena exprese genů (MRNA) vstupujících do řízení metabolismu tukové tkáně.
- MeSH
- inzulinová rezistence MeSH
- pohybová aktivita MeSH
- polymerázová řetězová reakce MeSH
- regulace genové exprese MeSH
- tuková tkáň MeSH
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- biologie
- endokrinologie
- diabetologie
- vnitřní lékařství
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
