Staphylococcal toxic shock syndrome (TSS) is a severe multisystemic disease caused by toxigenic strains of Staphylococcus aureus. The National Reference Laboratory (NRL) for Staphylococci has been involved in this issue since 1983.The article summarizes basic characteristics of 42 strains of S. aureus from suspected cases of TSS referred to the NRL in 2012 to 2015 and laboratory confirmed by toxigenicity testing. Half of these strains were identified as the cause of menstrual TSS, and the other half of them were responsible for complications of staphylococcal infections, most often pyoderma.
Stafylokokový syndrom toxického šoku (STŠ) je závažné multisystémové onemocnění, vyvolané toxigenními kmeny Staphylococcus aureus. V NRL pro stafylokoky CEM-SZÚ se této problematice věnujeme od roku 1983. V článku jsou uvedeny základní charakteristiky 42 kmenů S. aureus, které nám byly v průběhu let 2012–2015 zaslány v souvislosti se STŠ, u kterých jsme mohli potvrdit klinickou diagnózu průkazem toxigenity. Polovina (21) případů byla menstruální formy, zbývající byly komplikací různých stafylokokových infekcí, nejčastěji pyodermií.
- MeSH
- Bacterial Toxins immunology isolation & purification MeSH
- Feminine Hygiene Products * adverse effects MeSH
- Adult MeSH
- Enterotoxins immunology isolation & purification MeSH
- Infant MeSH
- Humans MeSH
- Menstruation MeSH
- Adolescent MeSH
- Infant, Newborn MeSH
- Otorhinolaryngologic Surgical Procedures adverse effects MeSH
- Postoperative Complications MeSH
- Registries MeSH
- Aged MeSH
- Shock, Septic * epidemiology etiology mortality MeSH
- Staphylococcal Infections * diagnosis epidemiology mortality MeSH
- Staphylococcus aureus immunology isolation & purification pathogenicity MeSH
- Superantigens immunology isolation & purification MeSH
- Check Tag
- Adult MeSH
- Infant MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Infant, Newborn MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Case Reports MeSH
- Geographicals
- Czech Republic MeSH
Seven coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococci assigned tentatively as Staphylococcus petrasii were investigated in this study in order to elucidate their taxonomic position. All strains were initially shown to form a genetically homogeneous group separated from remaining species of the genus Staphylococcus by using a repetitive sequence-based PCR fingerprinting with the (GTG)5 primer. Phylogenetic analysis based on 16S rRNA gene, hsp60, rpoB, dnaJ, gap and tuf sequences showed that the group is closely related to Staphylococcus petrasii but separated from the three hitherto known subspecies, S. petrasii subsp. petrasii, S. petrasii subsp. croceilyticus and S. petrasii subsp. jettensis. Further investigation using automated ribotyping, MALDI-TOF mass spectrometry, fatty acid methyl ester analysis, DNA-DNA hybridization and extensive biotyping confirmed that the analysed group represents a novel subspecies within S. petrasii, for which the name Staphylococcus petrasii subsp. pragensis subsp. nov. is proposed. The type strain is NRL/St 12/356(T) ( = CCM 8529(T) = LMG 28327(T)).
- MeSH
- Genes, Bacterial MeSH
- Chaperonin 60 genetics MeSH
- DNA, Bacterial genetics MeSH
- Phylogeny * MeSH
- Nucleic Acid Hybridization MeSH
- Humans MeSH
- Fatty Acids chemistry MeSH
- Molecular Sequence Data MeSH
- Oxidoreductases genetics MeSH
- Ribotyping MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Staphylococcus classification genetics isolation & purification MeSH
- Base Composition MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
Kmeny Staphylococcus aureus s produkcí Pantonova-Valentinova leukocidinu patří k velice virulentním etiologickým agens lidských infekcí. Vyvolávají závažná kožní a systémová onemocnění. V příspěvku je stručně popsán průběh onemocnění kožní infekce na bradě 36letého muže, kterou způsobil kmen MRSA s produkcí Pantonova- Valentinova leukocidinu.
Panton-Valentine leukocidin (PVL) producing strains of Staphylococcus aureus are highly virulent causative agents of human infections. They cause severe skin and systemic diseases. A brief case report is presented of a 36- year-old man with a skin infection on the chin due to a PVL-positive MRSA strain.
- MeSH
- Bacterial Toxins isolation & purification adverse effects MeSH
- Adult MeSH
- Exotoxins isolation & purification adverse effects MeSH
- Skin Diseases, Infectious * drug therapy microbiology MeSH
- Clindamycin administration & dosage MeSH
- Amoxicillin-Potassium Clavulanate Combination administration & dosage MeSH
- Leukocidins * isolation & purification adverse effects MeSH
- Humans MeSH
- Methicillin-Resistant Staphylococcus aureus * isolation & purification drug effects MeSH
- Drug Resistance, Multiple, Bacterial MeSH
- Staphylococcal Skin Infections * drug therapy microbiology MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Case Reports MeSH
