The protective effect of Enterococcus faecium EFAL41 on chicken's caecum in relation to the TLR (TLR4 and TLR21) activation and production of luminal IgA challenged with Campylobacter jejuni CCM6191 was assessed. The activation of MIF, IFN-β, MD-2 and CD14 was followed-up after bacterial infection. Day-old chicks (40) were divided into four groups (n = 10): control (C), E. faecium AL41 (EFAL41), C. jejuni (CJ) and combined E. faecium AL41+C. jejuni (EFAL41+CJ). Relative mRNA expression of TLR4, TLR21 and CD14 was upregulated in the probiotic strain and infected (combined) group on day 4 and 7 post infection (p.i.). The caecal relative MD-2 mRNA expression was upregulated on day 4 p.i. in the EFAL41+CJ and CJ groups. MIF and IFN-β reached the highest levels in the combined groups on day 7 p.i. The concentration of the sIgA in intestinal flush was upregulated in EFAL41+CJ group on day 4 p.i. The results demonstrated that E. faecium EFAL41 probiotic strain can modulate the TLRs expression and modify the activation of MIF, IFN-β, MD-2 and CD14 molecules in the chickens caecum challenged with C. jejuni CCM 6191. The counts of EFAL41 were sufficient and high, similarly the counts of enterococci in both, caecum and faeces but without reduction of Campylobacter counts.
- MeSH
- antigeny CD14 genetika imunologie MeSH
- Campylobacter jejuni růst a vývoj MeSH
- cékum imunologie mikrobiologie MeSH
- Enterococcus faecium růst a vývoj imunologie MeSH
- feces mikrobiologie MeSH
- imunoglobulin A sekreční genetika MeSH
- interakce hostitele a patogenu MeSH
- interferon beta genetika imunologie MeSH
- kampylobakterové infekce dietoterapie imunologie mikrobiologie veterinární MeSH
- kur domácí MeSH
- lymfocytární antigen 96 genetika imunologie MeSH
- nemoci drůbeže dietoterapie genetika imunologie mikrobiologie MeSH
- novorozená zvířata MeSH
- probiotika farmakologie MeSH
- protein - isoformy genetika imunologie MeSH
- receptory imunologické genetika imunologie MeSH
- regulace genové exprese MeSH
- signální transdukce MeSH
- toll-like receptor 4 genetika imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Lactobacillus, Bifidobacterium and Enterococcus cultures are increasingly used as probiotics for humans and pigs. The aim of this study was to investigate if co-cultivation of porcine and human neutrophils with probiotics can lead to increased apoptosis in vitro. Ten adult Large white pigs and 10 healthy human donors were used in this study. Neutrophils were isolated by dextran sedimentation and cultivated with and without the lactic acid bacteria Bifidobacterium bifidum, Lactobacillus rhamnosus, and Enterococcus faecium for 2, 4, 24 and 48 h. Early and late apoptosis was measured using flow cytometry, and cell lysis was detected based on lactate dehydrogenase activity (LDH). A significant (P < 0.05; P < 0.01) increase in apoptotic neutrophils and LDH was observed at 24 h and 48 h in vitro. All probiotics exerted their greatest effects on the early apoptosis of porcine neutrophils, while the effects of L. rhamnosus were most pronounced on late apoptosis and those of B. bifidumon LDH release of human neutrophils. The increased neutrophil apoptosis caused by probiotic bacteria can be beneficial for more efficient efferocytosis and faster resolution of inflammation and tissue regeneration. In conclusion, we have demonstrated that the interaction of B. bifidum, L. rhamnosus,and E. faecium with human and porcine neutrophils leads to their apoptosis.
- MeSH
- apoptóza MeSH
- Bifidobacterium bifidum fyziologie imunologie MeSH
- Enterococcus faecium fyziologie imunologie MeSH
- Lacticaseibacillus rhamnosus fyziologie imunologie MeSH
- lidé MeSH
- neutrofily MeSH
- probiotika * aplikace a dávkování farmakologie metabolismus MeSH
- průtoková cytometrie metody MeSH
- Sus scrofa MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- MeSH
- antibakteriální látky farmakologie klasifikace terapeutické užití MeSH
- bakteriální infekce farmakoterapie mortalita terapie MeSH
- bakteriální léková rezistence účinky léků MeSH
- Enterococcus faecium imunologie patogenita účinky léků MeSH
- Enterococcus imunologie patogenita účinky léků MeSH
- gramnegativní bakterie patogenita účinky léků MeSH
- grampozitivní bakterie patogenita účinky léků MeSH
- lidé MeSH
- methicilin rezistentní Staphylococcus aureus imunologie patogenita účinky léků MeSH
- mnohočetná bakteriální léková rezistence účinky léků MeSH
- morbidita MeSH
- mortalita MeSH
- polymyxiny farmakologie terapeutické užití MeSH
- Check Tag
- lidé MeSH
