Indoxyl sulfate has been identified as a major factor in the dysregulation of several genes. It is classified as a poorly dialyzable uremic toxin and thus a leading cause in the poor survival rate of dialysis patients. A monocentric, prospective, open cohort study was performed in 43 male patients undergoing chronic renal replacement therapy in a single hemodialysis center. The aim of the study was to determine the influence of acetate- versus citrate-buffered dialysis fluids in hemodialysis (HD) and postdilution hemodiafiltration (HDF) settings on the elimination of indoxyl sulfate. Also, additional factors potentially influencing the serum concentration of indoxyl sulfate were evaluated. For this purpose, the predialysis and postdialysis concentration ratio of indoxyl sulfate and total protein was determined. The difference was of 1.15 (0.61; 2.10), 0.89 (0.53; 1.66), 0.32 (0.07; 0.63), and 0.44 (0.27; 0.77) μmol/g in acetate HD and HDF and citrate HD and HDF, respectively. Acetate HD and HDF were superior when concerning IS elimination when compared to citrate HD and HDF. Moreover, residual diuresis was determined as the only predictor of lower indoxyl sulfate concentration, suggesting that it should be preserved as long as possible. This trial is registered with EU PAS Register of Studies EUPAS23714.

• MeSH
• acetáty farmakologie   MeSH
• dialýza ledvin metody   MeSH
• dialyzační roztoky chemie   farmakologie   MeSH
• hemodiafiltrace metody   MeSH
• hydrogenuhličitany MeSH
• indican krev   farmakokinetika   MeSH
• kyselina citronová krev   farmakologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• nemoci ledvin terapie   MeSH
• prospektivní studie MeSH
• senioři MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky MeSH

• MeSH
• acylkarnitin krev   MeSH
• časové faktory MeSH
• chronická renální insuficience * krev   MeSH
• dialýza ledvin metody   MeSH
• estery kyseliny sírové krev   MeSH
• indican krev   MeSH
• kresoly krev   MeSH
• lidé MeSH
• metabolom MeSH
• methylaminy krev   MeSH
• prospektivní studie MeSH
• větvené aminokyseliny krev   MeSH
• Check Tag
• lidé MeSH

• MeSH
• albuminurie MeSH
• biologické toxiny škodlivé účinky   MeSH
• gastrointestinální trakt patofyziologie   MeSH
• indican krev   MeSH
• lidé MeSH
• randomizované kontrolované studie jako téma MeSH
• renální insuficience * krev   mikrobiologie   MeSH
• rizikové faktory MeSH
• střevní mikroflóra * účinky léků   MeSH
• synbiotika * MeSH
• Check Tag
• lidé MeSH

Pathogenesis of adenine-induced chronic renal failure may involve inflammatory, immunological and/or oxidant mechanisms. Gum arabic (GA) is a complex polysaccharide that acts as an anti-oxidant which can modulate inflammatory and/or immunological processes. Therefore, we tested here the effect of GA treatment (15 % in the drinking water for 4 weeks) in plasma and urine of rats, on a novel cytokine that has been shown to be pro-inflammatory, viz, DNA-binding high-mobility group box-1 protein (HMGB1). Adenine (0.75 % in the feed, 4 weeks) significantly increased indoxyl sulphate, urea and creatinine concentrations in plasma, and significantly decreased the creatinine clearance. GA significantly abated these effects. The concentrations of HMGB1 in urine before the start of the experiment were similar in all four groups. However, 24 h after the last treatment, adenine treatment increased significantly the concentration of HMGB1 when compared with the control. GA treatment did not affect the HMGB1 concentration in urine. Moreover, the concentration of HMGB1 in plasma obtained 24 h after the last treatment in rats treated with adenine was drastically reduced compared with the control group. This may explain its significant rise in urine. In conclusion, HMGB1 can be considered a potentially useful biomarker in adenine induced CRF and its treatment.

• MeSH
• adenin * MeSH
• arabská guma farmakologie   MeSH
• biologické markery krev   moč   MeSH
• časové faktory MeSH
• chronické selhání ledvin chemicky indukované   farmakoterapie   metabolismus   patofyziologie   MeSH
• indican krev   MeSH
• kreatinin krev   MeSH
• ledviny účinky léků   metabolismus   patofyziologie   MeSH
• mediátory zánětu krev   metabolismus   moč   MeSH
• močovina krev   MeSH
• modely nemocí na zvířatech MeSH
• potkani Wistar MeSH
• protein HMGB1 krev   metabolismus   moč   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH