In this study, we were interested in the association of attenuated mutants of Salmonella enterica serovar Enteritidis with subpopulations of porcine white blood cells (WBC). The mutants included those with inactivated aroA, phoP, rfaL, rfaG, rfaC and fliC genes and a mutant with five major pathogenicity islands removed (ΔSPI1-5 mutant). Using flow cytometry, we did not observe any difference in the interactions of the wild-type S. Enteritidis, aroA and phoP mutants with WBC. ΔSPI1-5 and fliC mutants had a minor defect in their association with granulocytes and monocytes, but not with T- or B-lymphocytes. All three rfa mutants associated with granulocytes, monocytes and B-lymphocytes more than the wild-type S. Enteritidis did. Electron microscopy confirmed that the association correlated with the intracellular presence of S. Enteritidis and that the Salmonella-containing vacuole in the WBC infected with the rfa mutants, unlike all other strains, did not develop into a spacious phagosome. Intact lipopolysaccharide, but not the type III secretion system encoded by SPI-1, SPI-2 or the flagellar operon, is important for the initial interaction of S. Enteritidis with porcine leukocytes. This information can be used for the design of live Salmonella vaccines preferentially targeting particular cell types including cancer or tumor cells.
- MeSH
- bakteriální geny genetika MeSH
- glykosyltransferasy genetika MeSH
- leukocyty mikrobiologie ultrastruktura MeSH
- mutace genetika MeSH
- nemoci prasat imunologie mikrobiologie patologie MeSH
- prasata MeSH
- Salmonella enteritidis enzymologie genetika imunologie MeSH
- salmonelová infekce u zvířat imunologie mikrobiologie patologie MeSH
- salmonelové vakcíny imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
PURPOSE: To confirm and define a molecular basis for a case of mucolipidosis type IV (ML IV) with an extremely atypical phenotype pattern. DESIGN: Observational case report of a patient with ML IV with disease progression restricted to ocular symptoms. METHODS: Complete ophthalmologic and neurologic examination. Ultrastructural examination of white blood cells, skin, conjunctiva, and corneal epithelium. The MCOLN1 gene was sequenced from cDNA and the proportion of splicing variants were assessed by quantitative allele-specific polymerase chain reaction. RESULTS: Absence of any neurological abnormalities. Retinal pathologic features were the main cause of visual disability: low visual acuity and cloudy corneas since 2 years of age, progressive decrease in visual acuity since the age of 9 years. Ultrastructural examination showed storage lysosomes filled with either concentric membranes or lucent precipitate in corneal and conjunctive epithelia and in vascular endothelium. Cultured fibroblasts were free of any autofluorescence. Sequencing of the MCOLN1 gene identified compound heterozygosity for D362Y and A-->T transition leading to the creation of a novel donor splicing site and a 4-bp deletion from exon 13 at the mRNA level. Both normal and pathologic splice forms were detected in skin fibroblasts and leukocytes, with the normal form being more abundant. CONCLUSIONS: The case of this patient with ML IV is unique and is characterized by a curious lack of generalized symptoms. In this patient, the disorder was limited to the eyes and appeared without the usual psychomotor deterioration. The resulting phenotype is the mildest seen to date.
- MeSH
- alternativní sestřih genetika MeSH
- degenerace retiny genetika patologie MeSH
- dítě MeSH
- elektroretinografie MeSH
- epitelové buňky ultrastruktura MeSH
- fenotyp MeSH
- fibroblasty ultrastruktura MeSH
- financování organizované MeSH
- kationtové kanály TRPM genetika MeSH
- kůže ultrastruktura MeSH
- leukocyty ultrastruktura MeSH
- lidé MeSH
- lyzozomy genetika ultrastruktura MeSH
- messenger RNA genetika MeSH
- mukolipidózy genetika patologie MeSH
- mutace MeSH
- mutační analýza DNA MeSH
- nemoci rohovky genetika patologie MeSH
- nemoci spojivky genetika patologie MeSH
- polymerázová řetězová reakce MeSH
- rohovkový epitel ultrastruktura MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- MeSH
- Candida albicans MeSH
- dítě MeSH
- dospělí MeSH
- leukocyty patologie ultrastruktura MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- neutrofily enzymologie MeSH
- počet leukocytů MeSH
- trichomonádová vaginitida etiologie krev MeSH
- Trichomonas vaginalis izolace a purifikace krev MeSH
- vagina parazitologie patologie MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- ženské pohlaví MeSH
- MeSH
- histocytochemie MeSH
- leukocyty ultrastruktura MeSH
- lidé MeSH
- trijodthyronin MeSH
- Check Tag
- lidé MeSH
- MeSH
- leukocyty ultrastruktura MeSH
- lidé MeSH
- trijodthyronin MeSH
- vazebná místa MeSH
- Check Tag
- lidé MeSH
It was demonstrated that the tissue reaction around the developing C. bovis in neonatally infected calves (4 days after birth) differs quantitatively and partly also qualitatively from the reaction in calves infected at the age of several months. In neonatal infection, the foci caused by cysticerci were 3-4-times smaller on day 14 p.i., and after 34 days, the cysts were quite transparent and similar to those occurring in older calves not before 42-50 days p.i. The tissue eosinophilia was lacking and the activation of fibroblasts and collagen formation was markedly weak. There was a surprisingly low number of cells of lymphoid type. The growth of the cysticercus and its morphological differentiation was only insignificantly more rapid. The serological tests (IHA, MPA, IFR) repeated four times (10-34 days p.i.). were negative in one calf, whereas in the other one, slaughtered on day 14 p.i., positive results were obtained only in IFR (1:40).
- MeSH
- časové faktory MeSH
- cysticercus cytologie MeSH
- cysticerkóza imunologie parazitologie patologie veterinární MeSH
- granulom patologie MeSH
- kolagen MeSH
- leukocyty ultrastruktura MeSH
- makrofágy ultrastruktura MeSH
- nekróza MeSH
- nemoci skotu imunologie parazitologie patologie MeSH
- novorozená zvířata parazitologie MeSH
- skot MeSH
- stárnutí MeSH
- svaly parazitologie MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
