• MeSH
• antibakteriální látky MeSH
• biotransformace MeSH
• energetický metabolismus MeSH
• glukosa MeSH
• kultivační média MeSH
• kultivované buňky MeSH
• Streptomyces MeSH

• MeSH
• Aspergillus niger metabolismus   MeSH
• beta-glukosidasa chemie   metabolismus   MeSH

• MeSH
• aceton metabolismus   MeSH
• beta-glukosidasa metabolismus   MeSH
• katalýza MeSH

• MeSH
• Ascomycota enzymologie   MeSH
• beta-glukosidasa analýza   fyziologie   MeSH
• biodegradace MeSH
• celulosa metabolismus   MeSH
• kultivační média MeSH

Zm-p60.1 is maize cDNA coding cytokinin-glucoside specific beta-glucosidase. Indigogenic method was used for histochemical localization of Zm-p60.1 beta-glucosidase activity in various developmental stages of transgenic tobacco anthers. Expression of Zm-p60.1 cDNA in T7 tobacco plants is controlled by the CaMV 35S promoter. Another type of tobacco transformant expresses Zm-p60.1 under the control of LAT 52 promoter. Histochemical detection has proved different patterns of beta-glucosidase activity during tobacco pollen development in these two types of transformants. Zm-p60.1 beta-glucosidase activity had not direct influence on pollen germinability.

• MeSH
• beta-glukosidasa genetika   MeSH
• DNA primery genetika   MeSH
• exprese genu MeSH
• geneticky modifikované rostliny MeSH
• jedovaté rostliny MeSH
• kukuřice setá enzymologie   genetika   růst a vývoj   MeSH
• messenger RNA genetika   metabolismus   MeSH
• pyl enzymologie   genetika   růst a vývoj   MeSH
• RNA rostlin genetika   metabolismus   MeSH
• sekvence nukleotidů MeSH
• tabák MeSH
• transformace genetická MeSH

• MeSH
• Bacteria enzymologie   MeSH
• beta-glukosidasa analýza   MeSH
• celulosa metabolismus   MeSH
• houby enzymologie   MeSH
• kinetika MeSH

• MeSH
• beta-glukosidasa genetika   chemie   metabolismus   MeSH
• cystein genetika   MeSH
• kukuřice setá enzymologie   MeSH
• mutageneze cílená MeSH
• vztahy mezi strukturou a aktivitou genetika   MeSH

A new targeting potentially intravenous conjugate Amphotericin B (AMB)-star poly(ethylene glycol) (sPEG) (M=25,160) has been synthesized and characterized. It contains a beta-d-glucopyranoside molecular switch which is sensitive to beta-glucosidases (E.C.3.2.1.21). The beta-glucosidase-catalyzed release of AMB from the polymeric carrier was proved in vitro by means of spectrophotometry and HPLC.

• MeSH
• amfotericin B analogy a deriváty   farmakologie   chemická syntéza   MeSH
• antifungální látky farmakologie   chemická syntéza   MeSH
• beta-glukosidasa metabolismus   MeSH
• chemické modely MeSH
• financování organizované MeSH
• katalýza MeSH
• lidé MeSH
• polyethylenglykoly farmakologie   chemická syntéza   MeSH
• racionální návrh léčiv MeSH
• spektrofotometrie ultrafialová MeSH
• vysokoúčinná kapalinová chromatografie MeSH
• Check Tag
• lidé MeSH

• MeSH
• Aspergillus niger enzymologie   MeSH
• beta-glukosidasa fyziologie   MeSH
• endopeptidasy fyziologie   MeSH
• genetická vazba MeSH
• polysacharidy MeSH
• Publikační typ
• srovnávací studie MeSH

The activity of the phytohormone cytokinin depends on a complex interplay of factors such as its metabolism, transport, stability, and cellular/tissue localization. O-glucosides of zeatin-type cytokinins are postulated to be storage and/or transport forms, and are readily deglucosylated. Transgenic tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) plants were constructed over-expressing Zm-p60.1, a maize beta-glucosidase capable of releasing active cytokinins from O- and N3-glucosides, to analyse its potential to perturb zeatin metabolism in planta. Zm-p60.1 in chloroplasts isolated from transgenic leaves has an apparent K(m) more than 10-fold lower than the purified enzyme in vitro. Adult transgenic plants grown in the absence of exogenous zeatin were morphologically indistinguishable from the wild type although differences in phytohormone levels were observed. When grown on medium containing zeatin, inhibition of root elongation was apparent in all seedlings 14 d after sowing (DAS). Between 14 and 21 DAS, the transgenic seedlings accumulated fresh weight leading later (28-32 DAS) to ectopic growths at the base of the hypocotyl. The development of ectopic structures correlated with the presence of the enzyme as demonstrated by histochemical staining. Cytokinin quantification showed that transgenic seedlings grown on medium containing zeatin accumulate active metabolites like zeatin riboside and zeatin riboside phosphate and this might lead to the observed changes. The presence of the enzyme around the base of the hypocotyl and later, in the ectopic structures themselves, suggests that the development of these structures is due to the perturbance in zeatin metabolism caused by the ectopic presence of Zm-p60.1.

• MeSH
• beta-glukosidasa fyziologie   metabolismus   MeSH
• cytokininy metabolismus   MeSH
• financování organizované MeSH
• geneticky modifikované rostliny anatomie a histologie   enzymologie   účinky léků   MeSH
• homeostáza MeSH
• kinetika MeSH
• kukuřice setá enzymologie   genetika   MeSH
• kultivační média MeSH
• kyselina abscisová metabolismus   MeSH
• kyseliny indoloctové metabolismus   MeSH
• tabák genetika   MeSH
• zeatin farmakologie   metabolismus   MeSH