Explants
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PURPOSE: To assess the impact of Acanthamoeba keratitis (AK) and amniotic membrane transplantation (AMT) in corneal explants on presence of two multifunctional endogenous lectins, i.e. galectins-1 and -7. METHODS: Ten corneal explants from AK patients (five with previous AMT and five controls without this treatment) and seven specimens of disease-free control cornea were processed by indirect fluorescent immunohistochemistry. RESULTS: Immunostaining for both galectins was obtained in the epithelium, stroma and the endothelial layer of all controls, with the strongest positivity in the epithelium. Significantly decreased intensity for galectin-1 was recorded in the epithelium of corneal explants from patients with AK and AMT. The signal for galectin-7 was significantly decreased in the epithelium of AK patients and normalized after AMT. CONCLUSIONS: AMT has a marked impact on presence of the two galectins in opposite directions, encouraging complete profiling for this family of endogenous effectors.
- MeSH
- akantamébová keratitida metabolismus chirurgie MeSH
- amnion transplantace MeSH
- biologické krytí * MeSH
- biologické markery metabolismus MeSH
- dospělí MeSH
- galektin 1 MeSH
- galektiny metabolismus MeSH
- imunohistochemie MeSH
- keratoplastika perforující metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- oční infekce parazitární diagnóza metabolismus chirurgie MeSH
- rohovka metabolismus chirurgie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- multicentrická studie MeSH
OBJECTIVE: Translation of the contact zone in articulating joints is an important component of joint kinematics, yet rarely investigated in a biological context. This study was designed to investigate how sliding contact areas affect cartilage mechanobiology. We hypothesized that higher sliding speeds would lead to increased extracellular matrix mechanical stress and the expression of catabolic genes. DESIGN: A cylindrical Teflon indenter was used to apply 50 or 100 N normal forces at 10, 40, or 70 mm/s sliding speed. Mechanical parameters were correlated with gene expressions using a multiple linear regression model. RESULTS: In both loading groups there was no significant effect of sliding speed on any of the mechanical parameters (strain, stress, modulus, tangential force). However, an increase in vertical force (from 50 to 100 N) led to a significant increase in extracellular matrix strain and stress. For 100 N, significant correlations between gene expression and mechanical parameters were found for TIMP-3 (r(2) = 0.89), ADAMTS-5 (r(2) = 0.73), and lubricin (r(2) = 0.73). CONCLUSIONS: The sliding speeds applied do not have an effect on the mechanical response of the cartilage, this could be explained by a partial attainment of the "elastic limit" at and above a sliding speed of 10 mm/s. Nevertheless, we still found a relationship between sliding speed and gene expression when the tissue was loaded with 100 N normal force. Thus despite the absence of speed-dependent mechanical changes (strain, stress, modulus, tangential force), the sliding speed had an influence on gene expression.
- Publikační typ
- časopisecké články MeSH
Cíl studie: Zjistit možný přímý regulační vliv estrogenu na expresi MMP-26 in vitro.Typ studie: Experimentální studie.Název a sídlo pracoviště: Gynekologicko-porodnická klinika, UP LF a FN, Olomouc, Česká republika,Gynekologicko-porodnická klinika, Univerzitní nemocnice, Lund, Švédsko.Metodika: Studovali jsme MMP-26 mRNA ve 14 vzorcích normální endometriální tkáně, získanýchz proliferační fáze menstruálního cyklu. Vzorky byly pět dnů kultivovány buď se samotnýmestradiolem, nebo s kombinací estradiolu a progesteronu. Kontrolní skupiny tvořily vzorkykultivované s etanolem. Exprese MMP-26 mRNA byla zjišťována ve zmražených vzorcích pomocíin situ hybridizace. Přítomnost estrogenových a progesteronových receptorů byla stanovovánaimunohistochemicky.Výsledky: Exprese MMP-26 mRNA byla nejvyšší v čerstvých (nekultivovaných) endometriálníchvzorcích. Intenzita signálu strmě klesala v obou studovaných i kontrolních skupinách běhemprvních dvou dnů kultivace a v dalších dnech dosahovala zanedbatelných hladin. Nukleárníintenzita estrogenových i progesteronových receptorů byla po pěti dnech kultivace na vysokéúrovni.Závěr: Neprokázali jsme přímý regulační vliv estrogenu na expresi MMP-26 mRNA in vitro.
Objective: To examine possible estrogen dependent endometrial expression of MMP-26 in vitro.Design: Experimental study.Setting: Department of Obstetrics and Gynecology of the Palacky University Medical School andUniversity Hospital, Olomouc, Czech Republic, Department of Obstetrics and Gynecology, UniversityHospital, Lund, Sweden.Methods: We studied MMP-26 mRNA in 14 normal endometrial samples obtained from the proliferativephase of the menstrual cycle. Samples were cultured for fi ve days either with estradiolalone or in combination with progesterone. Samples cultured with ethanol represented controlgroups. MMP-26 mRNA expression was examined in frozen samples using in situ hybridization.Immunohistochemistry was used to study the presence of estrogen and progesterone receptorsin endometrial explants.Results: MMP-26 mRNA expression was highest in fresh (non cultured) samples. Signal intensitydecreased during the fi rst two days of culture and was negligable in the following days. Nuclearintensity for estrogen and progesterone receptor was high after fi ve days of culture.Conclusion: We did not fi nd MMP-26 mRNA in vitro expression to be directly estrogen dependent.
