Kadlec, Robert* Dotaz Zobrazit nápovědu
Prebiotics are generally considered to promote the function or viability of probiotics via their fermentation, but their effect on the adherence of probiotics is still unclear. In this study, we examined the effect of 4 commercially available prebiotics [Orafti GR, Orafti P95, and Orafti Synergy (Beneo GmbH, Mannheim, Germany), and Vivinal (Friesland Foods Domo, Amersfoort, the Netherlands)] and 3 simple saccharides (glucose, galactose, and lactose) on the adherence of 5 probiotic type strains, 2 lactococci starter cultures, and 5 potential dairy probiotic strains from the Culture Collection of Dairy Microorganisms (Tábor, Czech Republic). Adherence was tested in microtiter plates on the following types of substrate: polystyrene alone and polystyrene coated with either porcine mucus or cocultures of the human colon cell lines Caco2 and HT29-MXT (1:9 ratio of HT29-MXT:Caco2). Adherence was evaluated as a change in fluorescence in the well of a microtiter plate. The most commonly observed effect (with a few exceptions) of prebiotics was decreased adherence of the tested strains observed on all types of substrate. The tested saccharides, which are part of the residual compounds of the used prebiotics, had a very similar effect-eliciting a decrease in adherence ability in the majority of the probiotic strains.
- MeSH
- Bifidobacterium izolace a purifikace MeSH
- Caco-2 buňky MeSH
- Enterococcus izolace a purifikace MeSH
- fermentace MeSH
- Lacticaseibacillus rhamnosus izolace a purifikace MeSH
- Lactobacillus acidophilus izolace a purifikace MeSH
- Lactococcus izolace a purifikace MeSH
- lidé MeSH
- mléčné výrobky mikrobiologie MeSH
- nádorové buněčné linie MeSH
- oligosacharidy aplikace a dávkování MeSH
- potravinářská mikrobiologie MeSH
- prasata MeSH
- prebiotika * MeSH
- probiotika * MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Německo MeSH
- Nizozemsko MeSH
UNLABELLED: An understanding of adherence ability is crucial in many areas, for example, in research on biofilms, evaluation of probiotics or in biotechnology. In all these analyses, the reproducible washing is very important in the prevention of false results. During washing, the force, direction of the flow, position of the pipette tip, number of washing cycles, type of washing solution and the way of removing the washing solution can be sources of inappropriate stress to attached cells. To overcome these problems, we here propose the use of high mass density solutions as flotation agents. As the density of bacteria is lower than that of the flotation solutions, nonattached or weakly attached bacteria are moved to the surface due to hydrostatic force. Caesium chloride, ammonium nitrate and sodium diatrizoate solutions, which are commonly used as FAs, were compared with a standard method of rinsing. Several concentrations of agents were used to investigate the optimal concentration and influence of hydrostatic pressure on adhered micro-organisms. We show that flotation is a rapid method for distinguishing between adhered and weakly attached or loosed cells with reproducible results. Due to its range of possible mass density concentration, the best FA was shown to be caesium chloride. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study that suggests using flotation agents to separate planktonic from adhered bacteria. When a high-density solution is used, buoyancy of bacteria ensures their segregation in the solution. Flotation agents could be used instead of washing procedure, which is inaccurate and hardly reproducible. High-density flotation agents could be used for more precise evaluation of bacterial adherence in many assays, such as research of biofilms or evaluation of probiotics.
Cíl: Porovnat efekt operace katarakty s konvenční fakoemulzifikací a femtosekundovým laserem asistované operace katarakty na rohovku pomocí bezkontaktního spekulárního endotelového mikroskopu. Soubor pacientů a metodika: V každé skupině 25 očí (25 pacientů) s provedenou operací katarakty s klasickou fakoemulzifikací a 25 očí (25 pacientů) s femtosekundovým laserem asistovanou operací katarakty. Počet endotelových buněk byl měřen předoperačně, 1 den, 1 týden a 1 měsíc po operaci. Výsledky: Úbytek endotelových buněk jsme porovnávali ve 3 sledovacích obdobích. První den po operaci byl úbytek ve skupině s femtosekundovým laserem průměrně 1, 1 %, ve skupině s klasickou fakoemulzifikací 3, 8 %. Týden po operaci byl úbytek ve skupině s femtolaserem 4, 5 %, ve skupině s klasickou fakoemulzifikací 6, 2 %. Měsíc po operaci byl úbytek ve skupině s femtolaserem 5, 1 %, ve skupině s konveční fakoemulzifikací byl úbytek 9, 3 %. Rozdíl mezi hodnotami úbytku endotelových buněk nebyl ani v jednom sledovacím období statisticky významný (indikátorem statistické významnosti byla hodnota P < 0,05). Závěr: Femtosekundovým laserem asistovaná operace katarakty snižuje trauma rohovky a úbytek endotelových buněk ve srovnání s konvenční fakoemulzifikací.
Purpose: Compare the effect of cataract surgery with conventional phacoemulsification and cataract surgery performed on the cornea assisted by femtolaser with the help of contactless endothelial microscope. The group of patients and methodics: In each group there were 25 eyes (25 patients) after the cataract surgery with conventional phacoemulsification and 25 eyes (25 patients) after cataract surgery performed on the cornea assisted by femtolaser with the help of contactless endothelial microscope. The number of endothelial cells was measured preoperatively, 1 day, 1 week and 1 month after the surgery Results: We compared the loss of endothelial cells during 3 various periods. The first day after the surgery we noticed an average decrease in number of endothelial cells by 1, 1% in the group with femtosecond laser, in the group with conventional phacoemulsification we noticed an average decrease of 3, 8 %. The first week after the surgery we noticed an average decrease in number of endothelial cells by 4, 5 % in the group with femtosecond laser, in the group with conventional phacoemulsification we noticed on average decrease of 6, 2 %. One month after surgery we noticed on average decrease in number of endothelial cells by 5, 1 % in the group with femtosecond laser, in the group with conventional phacoemulsification we noticed on average decrease of 9, 3 %. The difference between the average values in both groups was not at any point statistically significant (indication of statistically significance was value of P < 0. 05). Conclusion: Cataract surgery performed on the cornea assisted by femtolaser reduces trauma of the cornea and it reduces the lost of endothelial cells in comparison to conventional phacoemulsification.
- MeSH
- endoteliální buňky MeSH
- fakoemulzifikace * MeSH
- implantace nitrooční čočky metody MeSH
- katarakta * terapie MeSH
- laserová terapie * metody MeSH
- lidé MeSH
- nitrooční čočky MeSH
- oftalmologické chirurgické výkony MeSH
- rohovka * chirurgie patofyziologie MeSH
- rohovkový endotel fyziologie MeSH
- výsledky a postupy - zhodnocení (zdravotní péče) * statistika a číselné údaje MeSH
- Check Tag
- lidé MeSH
