LBP/BPIs are pattern recognition receptors that are often present in vertebrates and in invertebrates, and they play a defense role against pathogens. We have identified 1698 bp cDNA sequence from the Eisenia andrei earthworm with predicted amino acid sequence that shares homology with the LBP/BPI family (EaLBP/BPI). Sequence analysis of EaLBP/BPI proved the existence of two conserved domains with the potential ability to bind LPS. The predicted molecular mass of the EaLBP/BPI protein is 53.5 kDa, and its high basicity (pI 9.8) is caused by its high arginine content. Constitutive transcription of the Ealbp/bpi gene was shown in all tested tissues, with the highest level in coelomocytes and seminal vesicles; the lowest level was detected in the intestine. On the contrary, another earthworm LPS-binding molecule CCF (coelomic cytolytic factor) was expressed only in the intestine and coelomocytes. In E. andrei coelomocytes, the transcription of Ealbp/bpi gene was up-regulated in response to bacterial stimulation, reaching a maximum at 8 and 16 h post stimulation with Bacillus subtilis and Escherichia coli, respectively.
- MeSH
- fylogeneze MeSH
- hybridizace in situ MeSH
- kationické antimikrobiální peptidy genetika MeSH
- klonování DNA MeSH
- krevní proteiny genetika MeSH
- molekulární sekvence - údaje MeSH
- Oligochaeta genetika imunologie MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- bolesti zad * diagnóza etiologie terapie MeSH
- chronická nemoc MeSH
- diagnostické techniky neurologické normy MeSH
- diferenciální diagnóza * MeSH
- GABA analogy a deriváty terapeutické užití MeSH
- lidé MeSH
- neuralgie diagnóza etiologie farmakoterapie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- novinové články MeSH
- rozhovory MeSH
End-stage renal disease (ESRD) is a serious health problem worldwide. The high prevalence of cardiovascular diseases and chronic inflammation remains a major cause of morbidity and mortality in haemodialysed patients. Beside some external factors, genetic predisposition both to renal failure and poor prognosis has been assumed. We have collected a total of 1,014 haemodialysed patients and 2,559 unrelated healthy Caucasians. Single-nucleotide polymorphisms (SNPs) in genes for preproghrelin (GHRL), lipopolysaccharide-binding protein (LBP), HFE and MTHFR were genotyped. In the group of patients, significantly more carriers presented the MTHFR T667T (P = 0.002) and HFE Asp63Asp (P = 0.001) and Cys282Cys (P = 0.01) genotypes. The frequencies of individual SNPs within GHRL and LBP genes did not differ between the patients and controls. The trends in genotype frequencies did not differ between the subgroups of patients with different time on haemodialysis. Common variants in MTHFR and HFE could be a risk factor for all-cause ESRD development, but are not predictors for the survival on haemodialysis.
- MeSH
- chronické selhání ledvin genetika MeSH
- genetická predispozice k nemoci genetika MeSH
- ghrelin genetika MeSH
- jednonukleotidový polymorfismus genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- membránové glykoproteiny genetika MeSH
- membránové proteiny genetika MeSH
- methylentetrahydrofolátreduktasa (NADPH2) genetika MeSH
- MHC antigeny I. třídy genetika MeSH
- proteiny akutní fáze genetika MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- transportní proteiny genetika MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
... Diferenciální diagnostika -- Symptomatologie LBP a GER 4 -- As. MUDr. Z. Kalvach, CSc. ...
15 stran ; 22 cm
- MeSH
- gastroezofageální reflux MeSH
- lumbalgie MeSH
- praktické lékařství MeSH
- Publikační typ
- abstrakty MeSH
- sborníky MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- všeobecné lékařství
- algeziologie
- gastroenterologie
- NLK Publikační typ
- semináře
- brožury
