Trans-resveratrol, a well-known plant phenolic compound, has been intensively investigated due to its association with the so-called French paradox. However, despite its high pharmacological potential, trans-resveratrol has shown relatively low bioavailability. Trans-resveratrol is intensively metabolized in the intestine and liver, yielding metabolites that may be responsible for its high bioactivity. The aim of this study was to investigate and compare the metabolism of trans-resveratrol (tRes), cis-resveratrol (cRes) and dihydroresveratrol (dhRes) in an in vitro epithelial model using Caco-2 cell lines. Obtained metabolites of tRes, cRes and dhRes were analyzed by LC/MS Q-TOF, and significant differences in the metabolism of each compound were observed. The majority of tRes was transported unchanged through the Caco-2 cells, while cRes was mostly metabolized. The main metabolite of both cis- and trans-resveratrol observed as a result of colon microbial metabolism, dhRes, was metabolized almost completely, with only traces of the unchanged molecule being found. A sulphate conjugate was identified as the main metabolite of tRes in our model, while a glucuronide conjugate was the major metabolite of cRes and dhRes. Since metabolism of simple phenolics and polyphenols plays a crucial role in their bioavailability, detailed knowledge of their transformation is of high scientific value.

• MeSH
• biologická dostupnost MeSH
• Caco-2 buňky MeSH
• lidé MeSH
• permeabilita MeSH
• resveratrol chemie   farmakokinetika   MeSH
• stereoizomerie MeSH
• stilbeny chemie   farmakokinetika   MeSH
• střevní sliznice cytologie   metabolismus   mikrobiologie   MeSH
• vysokoúčinná kapalinová chromatografie MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Natural organic additives such as eggs, lard, resins, and oils have been added to mortars since ancient times, because the ancient builders knew of their positive effect on the mortar quality. The tradition of adding organic materials to mortars was commonly handed down only verbally for thousands years. However, this practice disappeared in the nineteenth century, when the usage of modern materials started. Today, one of the most recent topics in the industry of building materials is the reusing of natural organic materials and searching for the forgotten ancient recipes. The research of the old technological approaches involves currently the most advanced analytical techniques and methods. This paper is focussed on testing the possibility of identification of proteinaceous additives in historical mortars and model mortar samples containing blood, bone glue, curd, eggs and gelatine, by Fourier transform infrared (FTIR) and Raman spectroscopy, gas chromatography - mass spectrometry (GC-MS), matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS), liquid chromatography-electrospray ionisation-quadrupole-time of flight mass spectrometry (LC-ESI-Q-TOF MS) and enzyme-linked immunosorbent assay (ELISA). All these methods were applied to the mortar sample taken from the interior of the medieval (sixteenth century) castle in Namest nad Oslavou in the Czech Republic and their comparison contributed to the rough estimation of the protein additive content in the mortar. The obtained results demonstrate that only LC-ESI-Q-TOF MS, MALDI-TOF MS and ELISA have the sufficiently low detection limits that enable the reliable identification of collagens in historical mortars. Graphical abstract Proteomics analyses of historical mortars.

• MeSH
• dějiny 16. století MeSH
• ELISA metody   MeSH
• kolagen analýza   MeSH
• konstrukční materiály analýza   dějiny   MeSH
• krevní proteiny analýza   MeSH
• plynová chromatografie s hmotnostně spektrometrickou detekcí metody   MeSH
• proteiny analýza   MeSH
• Ramanova spektroskopie metody   MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice metody   MeSH
• spektroskopie infračervená s Fourierovou transformací metody   MeSH
• vejce analýza   MeSH
• želatina analýza   MeSH
• zvířata MeSH
• Check Tag
• dějiny 16. století MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• historické články MeSH
• srovnávací studie MeSH
• Geografické názvy
• Česká republika MeSH

Objective: To profile maternal plasma metabolome in spontaneous preterm birth. Method: In this retrospective case-control study, we have examined plasma of patient with preterm birth (between 22 and 36 weeks of pregnancy (n = 57)), with threatened preterm labor (between 23 and 36 weeks of pregnancy (n = 49)), and with term delivery (n = 25). Plasma samples were analysed using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) in positive and negative polarity modes. Results: We found 168 differentially expressed metabolites that were significantly distinct between study groups. We determined 51 metabolites using publicly available databases that could be subdivided into one of the five groups: amino acids, fatty acids, lipids, hormones, and bile acids. PLS-DA models, verified by SVM classification accuracy, differentiated preterm birth and term delivery groups. Conclusions: Maternal plasma metabolites are different between term and preterm parturitions. Part of them may be related with preterm labor, while others may be affected by gestational age or the beginning of labor. Metabolite profile can classify preterm or term delivery groups raising the potential of metabolome as a biomarker to identify high-risk pregnancies. Metabolomic studies are also a tool to detect individual compounds that may be further tested in targeted researches.

• MeSH
• dospělí MeSH
• gestační stáří MeSH
• lidé MeSH
• mladý dospělý MeSH
• multivariační analýza MeSH
• předčasná porodní činnost krev   MeSH
• předčasný porod krev   MeSH
• retrospektivní studie MeSH
• studie případů a kontrol MeSH
• těhotenství MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Hmotnostní spektrometrie je neodmyslitelnou součástí moderní proteomiky. Při analýzách proteinů, ale i jiných biochemických molekul, nacházejí uplatnění různé typy hmotnostních spektrometrů. Článek se zabývá porovnáním hmotnostních spektrometrů na principu MALDI-TOF (Matrix Assisted Laser Desorption Ionisation–Time of Flight) a LC-Q LC-Q-TOF (Liquid Chromatography Quadrupole Time of Flight) z různých hledisek.

Mass spectrometry is inseparable part of modern proteomics. Different types of mass spectrometers are involved in analysis of proteins or the other biomolecules. The article compares mass spectrometers based on principles MALDI-TOF (Matrix Assisted Laser Desorption Ionisation–Time of Flight) and LC-Q LC-Q-TOF (Liquid Chromatography Quadrupole Time of Flight) from different point of views.

• MeSH
• chemické techniky analytické MeSH
• chromatografie micelární elektrokinetická kapilární * metody   přístrojové vybavení   využití   MeSH
• metody pro přípravu analytických vzorků MeSH
• mezibuněčné signální peptidy a proteiny analýza   klasifikace   MeSH
• mikrobiologické techniky MeSH
• proteiny analýza   klasifikace   ultrastruktura   MeSH
• proteomika * metody   přístrojové vybavení   MeSH
• software MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice * metody   přístrojové vybavení   využití   MeSH
• Publikační typ
• práce podpořená grantem MeSH

Peptidy izolované z PBL lidské krve budou eluovány z molekul B27 pacientů s AS, dále pacientů s různými formami B27 asociovaných onemocnění a zdravých kontrol. Peptidové profily budou porovnány metodou MALDI-TOF a Edmanovou degradací (pool sequencing). PBL budou izolovány z krve nemocných s AS a zdravých jedinců. Získané peptidy budou eluovány z molekul B27 v obou vyšetřovaných skupinách. Individuální peptidy budou sekvenovány pomocí LC/MS a Q-FTMS. Zjištěné sekvence peptidů eluované z kapsy B27 molekuly u nemocných budou srovnány se sekvencemi publikovanými v v B27-peptide REGISTER (Lopez de Castro, Tissue Antigens 2004). Peptidové profily buněk ze sleziny B27 transgenních myší s ANKENT entesopatií budou porovnány s kontrolními profily. Výsledky budousrovnány s údaji získanými u pacientů s AS.; PBL will be isolated from human blood and peptides will be eluted from B27 molecules of AS patients and healthy control. The peptide profiles will be compared by MALDI-TOF and Edman degradation for pool sequencing. PBL will be isolated from human blood and peptides will be eluted from B27 molecules of AS patients and healthy control.Individual peptides will be sequenced by LC/MS and Q-FTMS. Pools of PBL obtained from several AS diseased and healthy individuals will be tested to examine which individualpeptide appears as different,and thus exert a strong representation. Method used for the analysis of individual peptides will be MALDI-TOF. Peptide profiles will be examined from spleen cells of B27 transgenic mice with the ANKENT enthesopathy disease and healthy controls. The overall results will be compared with those obtained from AS patients.

• MeSH
• ankylózující spondylitida prevence a kontrola   terapie   MeSH
• autoimunita MeSH
• HLA antigeny analýza   MeSH
• HLA-B27 antigen analýza   MeSH
• peptidy analýza   MeSH

• Konspekt
• Biochemie. Molekulární biologie. Biofyzika
• NLK Obory
• alergologie a imunologie
• biologie
• revmatologie
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR

In the present study, the metabolic profile of amlodipine, a well-known calcium channel blocker, was investigated employing liquid chromatography-mass spectrometric (LC/MS) techniques. Two different types of mass spectrometers - a triple-quadrupole (QqQ) and a quadrupole time-of-flight (Q-TOF) mass spectrometer - were utilized to acquire structural information on amlodipine metabolites. The metabolites were produced by incubation of amlodipine with primary cultures of rat hepatocytes. Incubations from rat hepatocytes were analyzed with LC-MS/MS, and 21 phase I and phase II metabolites were detected. Their product ion spectra were acquired and interpreted, and structures were proposed. Accurate mass measurement using LC-Q-TOF was used to determine the elemental composition of metabolites and thus to confirm the proposed structures of these metabolites. Mainly phase I metabolic changes were observed including dehydrogenation of the dihydropyridine core, as well as reactions of side chains, such as hydrolysis of ester bonds, hydroxylation, N-acetylation, oxidative deamination, and their combinations. The only phase II metabolite detected was the glucuronide of a dehydrogenated, deaminated metabolite of amlodipine. We propose several in vitro metabolic pathways of amlodipine in rat, based on our analysis of the metabolites detected and characterized.

• MeSH
• acetylace MeSH
• amlodipin farmakokinetika   chemie   metabolismus   MeSH
• blokátory kalciových kanálů farmakokinetika   chemie   metabolismus   MeSH
• chromatografie kapalinová MeSH
• deaminace MeSH
• hepatocyty cytologie   metabolismus   MeSH
• hmotnostní spektrometrie s elektrosprejovou ionizací metody   MeSH
• hydroxylace MeSH
• I. fáze biotransformace MeSH
• II. fáze biotransformace MeSH
• krysa rodu rattus MeSH
• kultivované buňky MeSH
• molekulární struktura MeSH
• oxidace-redukce MeSH
• potkani Wistar MeSH
• tandemová hmotnostní spektrometrie metody   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH