1. elektronické vydání 1 online zdroj (67 stran)
Stopové kovy sú stredobodom záujmu mnohých vedeckých tímov pracujúcich v rôznych, či už výskumných alebo prevádzkových analytických laboratóriách. Pre ich spoľahlivú kvantifikáciu je použitie určitej vhodnej separačnej techniky takmer nevyhnutnosťou. Z mnohých separačných techník, ktoré možno v súčasnosti využiť na spoľahlivú separáciu a prekoncentráciu (ultra)stopových kovov patrí práve extrakcia s využitím teploty zákalu micelárnych roztokov (CPE) k značne sa rozvíjajúcim. V prvej časti monografie možno nájsť vysvetlenie základných princípov tejto extrakčnej techniky spolu s opisom experimentálnych parametrov, ktoré zohrávajú dôležitú úlohu pri vypracovávaní spoľahlivých CPE postupov a následne sú uvedené ukážky aplikácií v spojení so spektrometrickými metódami. V druhej časti monografie sú opísané nové prístupy k tejto extrakčnej technike, ktoré vedú predovšetkým k úspore času (ktorý je venovaný predúprave vzorky), vynechaniu metanolických roztokov (ktoré slúžia na zníženie viskozity tenzidom obohatenej fázy získanej po CPE separácii) a k zvýšeniu selektivity stanovenia (v prípade analýzy komplikovaných matríc). Najnovším smerovaním tejto extrakčnej techniky je spoľahlivá separácia a prekoncentrácia nanočastíc kovov v prítomnosti ich iónových špécií. Tejto téme je venovaná posledná kapitola v druhej časti monografie.
Registration of laser scans, or point clouds in general, is a crucial step of localization and mapping with mobile robots or in object modeling pipelines. A coarse alignment of the point clouds is generally needed before applying local methods such as the Iterative Closest Point (ICP) algorithm. We propose a feature-based approach to point cloud registration and evaluate the proposed method and its individual components on challenging real-world datasets. For a moderate overlap between the laser scans, the method provides a superior registration accuracy compared to state-of-the-art methods including Generalized ICP, 3D Normal-Distribution Transform, Fast Point-Feature Histograms, and 4-Points Congruent Sets. Compared to the surface normals, the points as the underlying features yield higher performance in both keypoint detection and establishing local reference frames. Moreover, sign disambiguation of the basis vectors proves to be an important aspect in creating repeatable local reference frames. A novel method for sign disambiguation is proposed which yields highly repeatable reference frames.
- MeSH
- Algorithms MeSH
- Datasets as Topic * MeSH
- Robotics MeSH
- Publication type
- Journal Article MeSH
The academic de.NBI Cloud offers compute resources for life science research in Germany. At the beginning of 2017, de.NBI Cloud started to implement a federated cloud consisting of five compute centers, with the aim of acting as one resource to their users. A federated cloud introduces multiple challenges, such as a central access and project management point, a unified account across all cloud sites and an interchangeable project setup across the federation. In order to implement the federation concept, de.NBI Cloud integrated with the ELIXIR authentication and authorization infrastructure system (ELIXIR AAI) and in particular Perun, the identity and access management system of ELIXIR. The integration solves the mentioned challenges and represents a backbone, connecting five compute centers which are based on OpenStack and a web portal for accessing the federation.This article explains the steps taken and software components implemented for setting up a federated cloud based on the collaboration between de.NBI Cloud and ELIXIR AAI. Furthermore, the setup and components that are described are generic and can therefore be used for other upcoming or existing federated OpenStack clouds in Europe.
- MeSH
- Biological Science Disciplines * MeSH
- Software * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Germany MeSH
The Differential Evolution (DE) is a widely used bioinspired optimization algorithm developed by Storn and Price. It is popular for its simplicity and robustness. This algorithm was primarily designed for real-valued problems and continuous functions, but several modified versions optimizing both integer and discrete-valued problems have been developed. The discrete-coded DE has been mostly used for combinatorial problems in a set of enumerative variants. However, the DE has a great potential in the spatial data analysis and pattern recognition. This paper formulates the problem as a search of a combination of distinct vertices which meet the specified conditions. It proposes a novel approach called the Multidimensional Discrete Differential Evolution (MDDE) applying the principle of the discrete-coded DE in discrete point clouds (PCs). The paper examines the local searching abilities of the MDDE and its convergence to the global optimum in the PCs. The multidimensional discrete vertices cannot be simply ordered to get a convenient course of the discrete data, which is crucial for good convergence of a population. A novel mutation operator utilizing linear ordering of spatial data based on the space filling curves is introduced. The algorithm is tested on several spatial datasets and optimization problems. The experiments show that the MDDE is an efficient and fast method for discrete optimizations in the multidimensional point clouds.
Currently, Biomedicine is characterised by a growing need for processing large amounts of data in real time. This leads to new requirements for information and communication technologies (ICT). Cloud computing offers a solution to these requirements and provides a lot of advantages such as cost savings, elasticity and scalability of using ICT. The aim of this paper is to explore the concept of cloud computing. Firstly, the forms of cloud computing are described. Secondly, the potential benefits and limitations of Biomedicine technology are discussed. Finally, the current (present) situation of using this technology in Biomedicine in the Czech Republic from an economic point of view is analysed.
- MeSH
- Biomedical Technology * MeSH
- Information Systems * MeSH
- Internet MeSH
- Computer Systems MeSH
- Delivery of Health Care * methods MeSH
- Diffusion of Innovation MeSH
- Software MeSH
- Information Storage and Retrieval MeSH
- Medical Informatics Applications MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
We synthesized statistical poly(2-isopropyl-2-oxazoline-co-2-butyl-2-oxazolines) (POXs) that are molecularly dissolved below their cloud point temperature in aqueous milieu and are incorporated into micellar nanoparticles of biocompatible Pluronic F127 (F127) after heating their solution above transition temperature, T(tr). A functional comonomer 2-(but-3-enyl)-2-oxazoline copolymerized into one of the POXs (polymer E) allows introduction of fenolic moieties and subsequent radionuclide labeling with iodine-125. Self-assembly of the polymer E with F127 leads to formation of radioactive nanoparticles with hydrodynamic diameter 20 nm in aqueous solution by heating to 37 °C. The nanoparticles are intended to be used as radioimaging tool in solid tumor diagnostics.
- MeSH
- Isotope Labeling MeSH
- Calorimetry MeSH
- Micelles MeSH
- Neoplasms MeSH
- Nanoparticles chemistry diagnostic use MeSH
- Oxazoles chemistry MeSH
- Poloxamer chemistry MeSH
- Iodine Radioisotopes chemistry MeSH
- Transition Temperature MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Brachytherapy represents effective local therapy of unresectable solid tumors with very few side effects. Radiolabeled thermoresponsive polymers offer almost noninvasive approach to brachytherapy applications. A radioiodinated, water-soluble, thermosensitive poly(N-vinylcaprolactam) (PVCL) polymer was prepared using two approaches. The direct copolymerization with N-methacryloyl-l-tyrosinamide, as well as end-capping of carboxy-terminated PVCL homopolymer with tyramine, were used. In both cases the product was successfully radiolabeled with (125)I. The obtained polymers demonstrate cloud-point temperature (TC) values in the range of 33-35°C in all the studied solvent systems (water, PBS (pH 7.4) and physiological saline solution). Above the cloud point temperature, the molecularly dissolved polymer is macroprecipitated from the solution. The TC values close to the human body temperature of this biocompatible poly(N-vinylcaprolactam) polymer makes it a promising material intended for local therapy of solid tumors.
- MeSH
- Biocompatible Materials chemistry MeSH
- Brachytherapy methods MeSH
- Caprolactam analogs & derivatives chemistry MeSH
- Humans MeSH
- Methacrylates chemistry MeSH
- Neoplasms radiotherapy MeSH
- Polymers chemistry MeSH
- Iodine Radioisotopes therapeutic use MeSH
- Solubility MeSH
- Temperature MeSH
- Materials Testing MeSH
- Tyrosine analogs & derivatives chemistry MeSH
- Water MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Parasitic protists of the genus Cytauxzoon are detected in a wide range of wild and domestic felids. Bobcats are a confirmed reservoir of Cytauxzoon felis in North America while domestic cats are susceptible hosts suffering from severe or fatal illness. Cytauxzoon infections are mainly reported from American felids and, recently, several sub-clinical and clinical findings were reported from European, Asian, and African felids. In 2014, the collection of organs of 4 Eurasian lynx and 12 wild cats from 11 Romanian localities was carried out to determine the prevalence and genetic diversity of Cytauxzoon spp. We detected an overall high prevalence of 62.5% in both species of wild felids; 50% in wild cats and 100% in Eurasian lynx. The phylogenetic analysis indicates 2 distinct clades of Cytauxzoon in felids, with all of our sequences clustering with sequences of Cytauxzoon sp./Cytauxzoon manul from Palaearctic felids. Further studies, development of new genetic markers, and experimental transmission studies are required for clarifying the taxonomy and life cycle of feline Cytauxzoon in the Old World.
- MeSH
- Arachnid Vectors parasitology MeSH
- Bayes Theorem MeSH
- Animals, Wild parasitology MeSH
- Felidae parasitology MeSH
- Felis parasitology MeSH
- Phylogeny MeSH
- Ticks parasitology MeSH
- Lynx parasitology MeSH
- Piroplasmida classification pathogenicity MeSH
- Prevalence MeSH
- Protozoan Infections, Animal epidemiology parasitology transmission MeSH
- Disease Reservoirs veterinary MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Europe epidemiology MeSH
- Romania epidemiology MeSH
The interest in the determination of different Sb species in natural waters is due to the fact that their toxicological and physiological behavior strongly depends on their chemical forms and oxidation states. The purpose of this article is to review and evaluate methods for Sb speciation in waters based on selective hydride generation of Sb (III) and on coupling of different separation techniques (liquid-liquid extraction, solid phase extraction, cloud point extraction, ion-exchange HPLC and GC) with atomic spectrometric methods (AAS, atomic emission spectrometry and atomic fluorescence spectrometry). This review covers the literature published over the period 1998-2006.
Thermoresponsive polymer micelles are promising drug and radionuclide carriers with a strong passive targeting effect into solid tumors. We have synthesized ABA triblock copolymers poly[2-methyl-2-oxazoline-block-(2-isopropyl-2-oxazoline-co-2-butyl-2-oxazoline)-block-2-methyl-2-oxazoline]. These polymers are molecularly dissolved in aqueous millieu below the cloud point temperature (CPT) of the thermoresponsive central block and above CPT form polymer micelles at CMC 5-10 × 10(-5) g · mL(-1) with diameter ≈200 nm. The phenolic moiety introduced into the copolymer allowed radionuclide labeling with iodine-125 ongoing in good yield with sufficient in vitro stability under model conditions.
- MeSH
- Hemolysis MeSH
- Humans MeSH
- Magnetic Resonance Spectroscopy MeSH
- Micelles MeSH
- Molecular Weight MeSH
- Oxazoles chemistry MeSH
- Polymers chemistry MeSH
- Radioisotopes administration & dosage MeSH
- Temperature MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
