Cílem je zjistit výskyt infekcí Helicobacter pylori /Hp/ u dětí bez příznaků a s různými formami onemocnění žaludku a duodena, srovnat antigeny Hp pro jejich využití v nepřímé diagnostice a vypracovat farmakoterapii infekce Hp.

• MeSH
• infekce vyvolané Helicobacter pylori terapie   diagnóza   MeSH

• Konspekt
• Buněčná biologie. Cytologie
• NLK Obory
• parazitologie
• infekční lékařství
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR

• MeSH
• aglutinace MeSH
• antibakteriální látky MeSH
• kultivační techniky MeSH
• Legionella izolace a purifikace   klasifikace   ultrastruktura   MeSH
• legionelóza farmakoterapie   MeSH
• mikrobiální testy citlivosti MeSH
• sérologické testy MeSH

• Konspekt
• Mikrobiologie
• NLK Obory
• bakteriologie
• chemie, klinická chemie
• NLK Publikační typ
• studie

Helicobacter pylori (HP) is considered a major gastric pathogen with oncogenic potential. The aim of this study was to determine whether HP is present in oropharyngeal lymphoid tissue and whether oropharyngeal HP strains carry virulence factor genes known to be involved in gastric carcinogenesis. The study included 104 subjects (41 patients with tonsillar carcinoma, 38 with chronic tonsillitis and 25 with obstructive sleep apnoea syndrome--OSAS). Detection of specific serum anti-HP antibodies was performed with an ELISA. The presence of HP in tissue was determined by culture and real-time PCR. Detection of virulence factors genes was also performed. Specific antibodies were found in 78.05% of tumour cases, 34.21% of chronic tonsillitis cases, and 72.0% of OSAS cases. The presence of HP in the tissue was detected in 73.91% of tonsillar tumours, 70.0% of tonsillitis cases, and 69.23% of OSAS specimens. The results of the virulence factor gene analysis showed the majority of the s1b (52.4%) and m2 (59.5%) alleles of vacA gene and limited abundance of cagA gene (12.5%). Results confirm that HP may colonise oropharyngeal lymphoid tissue. Oropharyngeal HP colonisation was frequently found in the oropharyngeal cancer group and in patients with benign oropharyngeal diseases. A virulence factor gene analysis showed differences from the predominant strains most commonly found in the stomach. The strains obtained from the oropharynx differed primarily by the lower abundance of the cagA gene and carried the less virulent vacA gene allele combination.

• MeSH
• antigeny bakteriální genetika   MeSH
• bakteriální proteiny genetika   MeSH
• chronická nemoc MeSH
• ELISA MeSH
• faktory virulence genetika   MeSH
• Helicobacter pylori genetika   MeSH
• infekce vyvolané Helicobacter pylori komplikace   diagnóza   imunologie   MeSH
• kvantitativní polymerázová řetězová reakce MeSH
• lidé MeSH
• lymfoidní tkáň mikrobiologie   MeSH
• nádory hlavy a krku komplikace   chirurgie   MeSH
• obstrukční spánková apnoe komplikace   chirurgie   MeSH
• orofarynx mikrobiologie   MeSH
• protilátky bakteriální imunologie   MeSH
• spinocelulární karcinom komplikace   chirurgie   MeSH
• techniky typizace bakterií MeSH
• tonzilární nádory komplikace   chirurgie   MeSH
• tonzilektomie MeSH
• tonzilitida komplikace   chirurgie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• Publikační typ
• abstrakt z konference MeSH

Helicobacter pylori has been recently detected in the oral cavity and oropharynx. However, the role it plays in oral and oropharyngeal pathogenesis remains unclear. The virulence of H. pylori strains can be distinguished according to the virulence factors genes carried. Our research has been focused on realtime PCR analysis of cagA and vacA genes of H. pylori strains in tonsils and tonsillar squamous cell cancer and their comparison with H. pylori strains obtained from the gastric mucosa of the same patients. Urea breath test (UBT) test was used to detect a gastric H. pylori infection in 20 patients with previously proven H. pylori in the oropharynx. Genotyping of H. pylori in gastric biopsies was performed in patients with positive gastric infection. Out of 20 patients positive for oropharyngeal H. pylori, 8 were positive for concurrent gastric H. pylori infection. In 6 of them gastric biopsies were obtained. Comparison of oropharyngeal and stomach H. pylori genotypes showed important differences. Four of 6 patients had different H. pylori strains in the oropharynx and stomach. The differences were found in cagA gene as well as in vacA gene. The finding of oral presence of H. pylori without concurrent stomach infection was confirmed using UBT. The results show that more than one H. pylori strain can be present in oropharynx and stomach in the same patient. The oropharyngeal infection seems to be independent to the gastric infection.

• MeSH
• antigeny bakteriální genetika   MeSH
• bakteriální proteiny genetika   MeSH
• genotyp MeSH
• Helicobacter pylori genetika   izolace a purifikace   MeSH
• infekce vyvolané Helicobacter pylori MeSH
• lidé MeSH
• orofarynx mikrobiologie   MeSH
• žaludek mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH
• srovnávací studie MeSH

• Publikační typ
• abstrakty MeSH

Helicobacter pylori (Hp) contributes to the development of gastric and extra-gastric diseases such as autoimmune thyroiditis (AT), and causes persistent life-long infection despite local and systemic immune response. We determined the specific cellular immune response to Hp antigens and PWM (control mitogen) in two groups of Hp infected patients--group A (n = 21), involving patients with autoimmune thyroiditis and group B (n = 13) of patients without AT--using modified lymphocyte transformation test before and after eradication therapy in comparison with healthy controls (group C, n = 15). Immune reactivity to the majority of Hp antigens (aHp, hHp, HpAg, CagA) was significantly lower in group B before eradication therapy in comparison with healthy Hp negative controls. A significant increase in immune reactivity was observed in group B to certain Hp antigens after successful eradication. The same levels (but insignificant) of immune reactivity were shown in group A. Our results indicate that Hp can cause the inhibition of the specific cellular immune response in Hp infected patients with or without autoimmune diseases such as AT, which can be abrogated by successful eradication of Hp. Lymphocyte transformation test appears to be a good tool for detection of immune memory cellular response in patients with Hp infection

• MeSH
• antigeny bakteriální imunologie   MeSH
• autoimunitní tyreoiditida imunologie   komplikace   MeSH
• dospělí MeSH
• financování organizované MeSH
• Helicobacter imunologie   MeSH
• imunologická tolerance MeSH
• infekce vyvolané Helicobacter pylori imunologie   klasifikace   MeSH
• lidé středního věku MeSH
• lidé MeSH
• lymfocyty imunologie   MeSH
• proliferace buněk MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH

Helicobacter pylori is a well-known gastric pathogen. It plays a major role in the pathogenesis of chronic gastritis, duodenal and gastric ulcers, adenocarcinoma and gastric lymphoma. HP infection is one of the most common bacterial infections worldwide. Recently, the oral cavity was proposed as an extragastric reservoir of HP infection. HP was detected by culture and PCR in both dental plaque and saliva. It is supposed that HP infection can cause the same immunological changes in the oropharyngeal mucosa as in gastric mucosa and can also contribute to the progression of oropharyngeal diseases. HP can induce production of different cytokines and regulatory molecules, which are suggested to play a role in carcinogenesis of the oropharynx. Only a few studies have explored the presence of HP in tonsillar and adenoid tissue, where MALT is present similar to the gastric mucosa. The results of these studies were inconsistent. The question of persistence of HP in tonsillar and adenoid tissue and its role in the pathogenesis of oropharyngeal diseases still remains unclear. In this review, recent findings about oral HP are considered. Possibilities of diagnostics of HP in oral specimens are discussed.

• MeSH
• adenoidy mikrobiologie   MeSH
• cytokiny imunologie   metabolismus   MeSH
• Helicobacter pylori patogenita   MeSH
• infekce vyvolané Helicobacter pylori diagnóza   imunologie   mikrobiologie   patofyziologie   MeSH
• krční mandle mikrobiologie   MeSH
• lidé MeSH
• nádory orofaryngu etiologie   mikrobiologie   MeSH
• orofarynx mikrobiologie   MeSH
• žaludeční sliznice mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• přehledy MeSH

• Publikační typ
• abstrakty MeSH

• Publikační typ
• abstrakt z konference MeSH