Q95392207
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Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Přeruš. str. : il. ; 30 cm
Vývoj nových experimentálních metod detekce a kvantitativního hodnocení důsledků anoxického poškození mozku. XXX XXX XXX
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- neurovědy
- radiologie, nukleární medicína a zobrazovací metody
- chemie, klinická chemie
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
Neural crest cells (NCCs) derive early in vertebrate ontogenesis from neural tube as a population of migratory cells with exquisite differentiation potential. Abnormalities in NCC behaviour are cause of debilitating diseases including cancers and a spectrum of neurocristopathies. Thanks to their multilineage differentiation capacity NCCs offer a cell source for regenerative medicine. Both these aspects make NCC biology an important issue to study, which can currently be addressed using methodologies based on pluripotent stem cells. Here we contributed to understanding the biology of human NCCs by refining the protocol for differentiation/propagation of NCClike cells from human embryonic stem cells and by characterizing the molecular and functional phenotype of such cells. Most importantly, we improved formulation of media for NCC culture, we found that poly-L-ornithine combined with fibronectin provide good support for NCC growth, we unravelled the tendency of cultured NCCs to maintain heterogeneity of CD271 expression, and we showed that NCCs derived here possess the capacity to react to BMP4 signals by dramatically up-regulating MSX1, which is linked to odontogenesis.
- MeSH
- adapalen MeSH
- biologické markery metabolismus MeSH
- buněčná diferenciace * účinky léků MeSH
- crista neuralis cytologie účinky léků metabolismus MeSH
- embryonální kmenové buňky cytologie účinky léků metabolismus MeSH
- fenotyp MeSH
- kostní morfogenetický protein 4 farmakologie MeSH
- lidé MeSH
- naftaleny metabolismus MeSH
- polymerázová řetězová reakce MeSH
- průtoková cytometrie MeSH
- transkripční faktor MSX1 metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Tooth absence and defects caused by various reasons are frequent events in humans. They are not life threatening but may bring about social consequences. Recent dentistry provides solutions in the form of prosthetics or dental implants; however, several complications and distinct limitations favour bioengineering of dental and periodontal structures. At least two types of cells (epithelial and mesenchymal) have to be recombined to produce a new functional tooth. Moreover, the tooth must be vascularized, innervated and properly anchored in the bone. To study these issues, different approaches have been established in both basic and applied research. In this review, recent strategies and techniques of tooth engineering are comprehensively summarized and discussed, particularly regarding manipulation using stem cells.
- MeSH
- implantace protézy MeSH
- kmenové buňky cytologie MeSH
- lidé MeSH
- tkáňové inženýrství metody trendy MeSH
- tkáňové podpůrné struktury MeSH
- výzkum * MeSH
- zuby fyziologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
A microdialysis technique was used to monitor changes in serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and dopamine (DA) in the extracellular space of the parabrachial nucleus (PBN) of rats to estimate the contribution of these neurotransmitter systems to the acquisition of conditioned taste aversion (CTA). A significant (280%) enhancement of 5-HT was found immediately after saccharin drinking (CS). I.p. injection of unconditioned stimulus LiCl alone (after water drinking) also increased level of 5-HT (200%). However, when saccharin intake was followed by injection of LiCl (CS-US pairing), no change in 5-HT was observed. 5-HIAA and DA were unaffected by any of the above treatments. Thus in spite of elevation of 5-HT in PBN following saccharin consumption alone (CS) or LiCl administration alone (US) no changes in 5-HT occurred after pairing of both stimuli (CS-US). Our work demonstrates that participation of 5-HT in acquisition of CTA appears to be unlikely, and also DA appears not to be engaged in this acquisition at all. At the level of the PBN 5-HT participates mainly in CS and/or US stimuli processing, where this phenomenon has close relationship to other important physiological mechanisms, involved in behavioral control. Such as anxiety, alimentation intake.
- MeSH
- chování zvířat MeSH
- chuť MeSH
- dopamin metabolismus MeSH
- financování organizované MeSH
- krysa rodu rattus MeSH
- kyselina hydroxyindoloctová metabolismus MeSH
- mikrodialýza metody MeSH
- operantní podmiňování fyziologie MeSH
- pons fyziologie MeSH
- potkani Long-Evans MeSH
- preference v jídle fyziologie MeSH
- serotonin metabolismus MeSH
- učení vyhýbat se fyziologie MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- autoimunitní tyreoiditida terapie MeSH
- celiakie komplikace MeSH
- dospělí MeSH
- lidé MeSH
- Takayasuova arteriitida terapie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- Publikační typ
- kazuistiky MeSH
- MeSH
- autoimunitní tyreoiditida MeSH
- celiakie MeSH
- dospělí MeSH
- lidé MeSH
- Takayasuova arteriitida MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
