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The embedding microbial fuel cell (MFC) into constructed wetlands (CW) to form CW-MFC bears the potential to obtain bioelectricity and a clean environment. In this study, a bibliometric analysis using VOSviewer based on Web of Science data was conducted to provide an overview by tracing the development footprint of this technology. The countries, institutions, authors, key terms, and keywords were tracked and corresponding mapping was generated. From 2012 to September 2020, 442 authors from 129 organizations in 26 countries published 135 publications in 42 journals with total citation of 3139 times were found. The key terms analysis showed four clusters: bioelectricity generation performance, mechanism study, refractory pollutants removal, and enhanced conventional contaminants removal. Further research themes include exploring the biochemical properties of electrochemically active bacteria, emerging contaminants removal, effective bioelectricity harvest and the use, and biosensor development as well as scaling-up for real field application. The bibliometric results provide valuable references and information on potential research directions for future studies.
MONOGRAPHS . xii -- PREAMBLE .xiii -- WHO DRAFTING GROUP ON ENVIRONMENTAL HEALTH -- CRITERIA FOR DIESEL FUEL AND EXHAUST EMISSIONS xix -- WHO TASK GROUP ON ENVIRONMENTAL HEALTH -- CRITERIA FOR DIESEL FUEL AND EXHAUST EMISSIONS xxi -- ENVIRONMENTAL HEALTH CRITERIA FOR DIESEL FUEL -- AND EXHAUST EMISSIONS .xxiii -- PARTA: DIESEL FUEL -- Ai. , PHYSICAL AND CHEMICAL PROPERTIES, -- AND ANALYTICAL METHODS . 10 -- A2.1 Identity . 10 -- A2.1.1 Fuel
Environmental health criteria ; 171
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- MeSH
- látky znečišťující vzduch MeSH
- ropa toxicita MeSH
- topné oleje MeSH
- výfukové emise vozidel toxicita MeSH
- vystavení vlivu životního prostředí MeSH
- znečištění ovzduší MeSH
- Konspekt
- Veřejné zdraví a hygiena
- NLK Obory
- environmentální vědy
- hygiena
- NLK Publikační typ
- publikace WHO
Air pollution caused by road traffic has an unfavorable impact on the environment and also on human health. It has previously been shown, that complete gasoline emissions lead to toxic effects in cell models originating from human airways. Here we focused on extractable organic matter (EOM) from particulate matter, collected from gasoline emissions from fuels with different ethanol content. We performed cytotoxicity evaluation, quantification of mucin and extracellular reactive oxygen species (ROS) production, DNA breaks detection, and selected gene deregulation analysis, after one and five days of exposure of human bronchial epithelial model (BEAS-2B) and a 3D model of the human airway (MucilAirTM). Our data suggest that the longer exposure had more pronounced effects on the parameters of cytotoxicity and mucin production, while the impacts on ROS generation and DNA integrity were limited. In both cell models the expression of CYP1A1 was induced, regardless of the exposure period or EOM tested. Several other genes, including FMO2, IL1A, or TNF, were deregulated depending on the exposure time. In conclusion, ethanol content in the fuels did not significantly impact the toxicity of EOM. Biological effects were mostly linked to xenobiotics metabolism and inflammatory response. BEAS-2B cells were more sensitive to the treatment.
- MeSH
- benzin * MeSH
- bronchy cytologie MeSH
- buněčné linie MeSH
- cytochrom P-450 CYP1A1 genetika MeSH
- epitelové buňky účinky léků metabolismus MeSH
- histony metabolismus MeSH
- interleukin-1alfa genetika MeSH
- látky znečišťující vzduch toxicita MeSH
- lidé MeSH
- oxygenasy genetika MeSH
- pevné částice toxicita MeSH
- reaktivní formy kyslíku metabolismus MeSH
- regulace genové exprese účinky léků MeSH
- TNF-alfa genetika MeSH
- výfukové emise vozidel toxicita MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
In this work, a novel cation exchange membrane, PSEBS SU22 was deployed in microbial fuel cells (MFCs) to examine system efficacy in line with membrane characteristics and inoculum source. It turned out that compared to a reference membrane (Nafion), employing PSEBS SU22 resulted in higher current density and electricity generation kinetics, while the electron recoveries were similar (19-28%). These outcomes indicated more beneficial ion transfer features and lower mass transfer-related losses in the PSEBS SU22-MFCs, supported by membrane water uptake, ion exchange capacity, ionic conductivity and permselectivity. By re-activating the membranes after (bio)foulant removal, PSEBS SU22 regained nearly its initial conductivity, highlighting a salient functional stability. Although the particular inoculum showed a clear effect on the microbial composition of the membrane biofouling layers, the dominance of aerobic species was revealed in all cases. Considering all the findings, the PSEBS SU22 seems to be promising for application in MFCs.
Modern vehicles equipped with Gasoline Direct Injection (GDI) engine have emerged as an important source of particulate emissions potentially harmful to human health. We collected and characterized gasoline exhaust particles (GEPs) produced by neat gasoline fuel (E0) and its blends with 15% ethanol (E15), 25% n-butanol (n-But25) and 25% isobutanol (i-But25). To study the toxic effects of organic compounds extracted from GEPs, we analyzed gene expression profiles in human lung BEAS-2B cells. Despite the lowest GEP mass, n-But25 extract contained the highest concentration of polycyclic aromatic hydrocarbons (PAHs), while i-But25 extract the lowest. Gene expression analysis identified activation of the DNA damage response and other subsequent events (cell cycle arrest, modulation of extracellular matrix, cell adhesion, inhibition of cholesterol biosynthesis) following 4 h exposure to all GEP extracts. The i-But25 extract induced the most distinctive gene expression pattern particularly after 24 h exposure. Whereas E0, E15 and n-But25 extract treatments resulted in persistent stress signaling including DNA damage response, MAPK signaling, oxidative stress, metabolism of PAHs or pro-inflammatory response, i-But25 induced changes related to the metabolism of the cellular nutrients required for cell recovery. Our results indicate that i-But25 extract possessed the weakest genotoxic potency possibly due to the low PAH content.
- MeSH
- benzin analýza toxicita MeSH
- biopaliva analýza toxicita MeSH
- buněčné linie MeSH
- butanoly analýza toxicita MeSH
- ethanol chemie MeSH
- genetická transkripce účinky léků MeSH
- látky znečišťující vzduch analýza toxicita MeSH
- lidé MeSH
- MAP kinasový signální systém účinky léků MeSH
- organické látky chemie toxicita MeSH
- oxidační stres účinky léků MeSH
- pevné částice toxicita MeSH
- plíce účinky léků patologie MeSH
- polycyklické aromatické uhlovodíky analýza toxicita MeSH
- poškození DNA MeSH
- stanovení celkové genové exprese MeSH
- výfukové emise vozidel analýza toxicita MeSH
- zánět chemicky indukované patologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
An analysis of the toxic effects of emissions should reflect real traffic conditions. The exhaust emissions of particulate matter from diesel engines strongly depend on their operating conditions, with low-speed, low-load "urban creep" conditions, common for truck traffic in heavily congested urban areas, being one of the worst. We aimed to detect the genotoxicity of organic extracts from particulate matter in the exhaust of the diesel engine Zetor 1505 running on diesel and biodiesel (B100) fuels at characteristic modes of extended "urban creep", typical for transit truck traffic in Prague, comparing the first 5 min of idling with extended (20-80 min) idling, full load after idle, "stabilized" full load, and 30% load. The diluted exhaust was sampled with high volume samplers on glass fiber fluorocarbon coated filters. The filters were extracted with dichloromethane and DNA damage was analyzed in A549 cells using comet assay, with the inclusion of formamidopyrimidine DNA glycosylase (FPG) and endonuclease III (ENDOIII) to recognize oxidized DNA bases. The cells were exposed to extractable organic matter (EOM) for 4 and 24 h at non-cytotoxic dose corresponding to 0.001 m3 of undiluted exhaust gas per ml cell media. At the 4 h exposure interval, all samples from B100 and diesel emissions induced DNA damage. EOM from the extended idle engine mode exerted the strongest genotoxic effect for both fuels. Twenty hours later, the cells exposed to diesel EOM exhibited a further increase of DNA strand breaks compared to the preceding interval. In contrast, DNA damage seemed to be fully repaired in cells treated with EOM derived from biodiesel B100. The preliminary results suggest that (i) diesel emissions are more genotoxic than the emissions from B100, (ii) biodiesel induced DNA lesions are repaired within 24 h.
- MeSH
- benzin analýza toxicita MeSH
- biopaliva analýza toxicita MeSH
- buňky A549 MeSH
- chemická frakcionace metody MeSH
- karcinogeny životního prostředí analýza toxicita MeSH
- kometový test MeSH
- lidé MeSH
- oxidace-redukce MeSH
- pevné částice toxicita MeSH
- pilotní projekty MeSH
- polycyklické aromatické uhlovodíky izolace a purifikace toxicita MeSH
- poškození DNA MeSH
- rozpouštědla MeSH
- těkavé organické sloučeniny izolace a purifikace toxicita MeSH
- viabilita buněk účinky léků MeSH
- výfukové emise vozidel analýza toxicita MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
Tumors can reprogram the functions of metabolic enzymes to fuel malignant growth; however, beyond their conventional functions, key metabolic enzymes have not been found to directly govern cell mitosis. Here, we report that glutamine synthetase (GS) promotes cell proliferation by licensing mitotic progression independently of its metabolic function. GS depletion, but not impairment of its enzymatic activity, results in mitotic arrest and multinucleation across multiple lung and liver cancer cell lines, patient-derived organoids and xenografted tumors. Mechanistically, GS directly interacts with the nuclear pore protein NUP88 to prevent its binding to CDC20. Such interaction licenses activation of the CDC20-mediated anaphase-promoting complex or cyclosome to ensure proper metaphase-to-anaphase transition. In addition, GS is overexpressed in human non-small cell lung cancer and its depletion reduces tumor growth in mice and increases the efficacy of microtubule-targeted chemotherapy. Our findings highlight a moonlighting function of GS in governing mitosis and illustrate how an essential metabolic enzyme promotes cell proliferation and tumor development, beyond its main metabolic function.
- MeSH
- glutaminsynthetasa MeSH
- licence MeSH
- lidé MeSH
- myši MeSH
- nádory plic * MeSH
- nemalobuněčný karcinom plic * MeSH
- proliferace buněk MeSH
- proteiny buněčného cyklu metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
This study used toxicogenomics to identify the complex biological response of human lung BEAS-2B cells treated with organic components of particulate matter in the exhaust of a diesel engine. First, we characterized particles from standard diesel (B0), biodiesel (methylesters of rapeseed oil) in its neat form (B100) and 30% by volume blend with diesel fuel (B30), and neat hydrotreated vegetable oil (NEXBTL100). The concentration of polycyclic aromatic hydrocarbons (PAHs) and their derivatives in organic extracts was the lowest for NEXBTL100 and higher for biodiesel. We further analyzed global gene expression changes in BEAS-2B cells following 4 h and 24 h treatment with extracts. The concentrations of 50 µg extract/mL induced a similar molecular response. The common processes induced after 4 h treatment included antioxidant defense, metabolism of xenobiotics and lipids, suppression of pro-apoptotic stimuli, or induction of plasminogen activating cascade; 24 h treatment affected fewer processes, particularly those involved in detoxification of xenobiotics, including PAHs. The majority of distinctively deregulated genes detected after both 4 h and 24 h treatment were induced by NEXBTL100; the deregulated genes included, e.g., those involved in antioxidant defense and cell cycle regulation and proliferation. B100 extract, with the highest PAH concentrations, additionally affected several cell cycle regulatory genes and p38 signaling.
- MeSH
- anotace sekvence MeSH
- benzin analýza toxicita MeSH
- biopaliva analýza toxicita MeSH
- bronchy cytologie účinky léků metabolismus MeSH
- epitelové buňky cytologie účinky léků metabolismus MeSH
- látky znečišťující vzduch analýza toxicita MeSH
- lidé MeSH
- oleje rostlin chemie MeSH
- pevné částice analýza toxicita MeSH
- polycyklické aromatické uhlovodíky analýza toxicita MeSH
- regulace genové exprese u rostlin * MeSH
- rostlinné proteiny genetika metabolismus MeSH
- signální transdukce MeSH
- stanovení celkové genové exprese MeSH
- transformované buněčné linie MeSH
- výfukové emise vozidel analýza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
Recent findings about the new roles of lactate have changed our understanding of this end product of glycolysis or fermentation that was once considered only a waste product. It is now well accepted that lactate acts as a signaling molecule and fuel source for cancer cells in a glucose-restricted environment. Moreover, lactate and lactate dehydrogenase are markers of poor prognosis of many cancers and regulate many functions of immune cells. The presence of lactate in the tumor microenvironment (TME) leads to polarization of the immunosuppressive phenotypes of dendritic cells and impairs the cytotoxic abilities of T cells and NK cells, and as such lactate is a major obstacle to immune-cell effector functions and the efficacy of cell-based immunotherapies. Emerging evidence suggests that lactate in the TME might be a novel therapeutic target to enhance the immunotherapeutic potential of cell-based therapies. This review describes our current understanding of the role of lactate in tumor biology, including its detrimental effects on cell-based immunotherapy in cancer. We also highlight how the role of lactate in the TME must be considered when producing cell therapies designed for adoptive transfer and describe how targeted modulation of lactate in the TME might boost immune-cell functions and positively impact cellular immunotherapy, with a focus on NK cell.
- MeSH
- buňky NK MeSH
- imunoterapie MeSH
- kyselina mléčná MeSH
- lidé MeSH
- nádorové mikroprostředí * MeSH
- nádory * terapie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
